| Project/Area Number |
25430003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Gunma University |
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Principal Investigator |
HOSOI Nobutake 群馬大学, 大学院医学系研究科, 講師 (90543570)
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| Co-Investigator(Renkei-kenkyūsha) |
KONNO Ayumu 群馬大学, 大学院医学系研究科, 講師 (40509048)
HIRAI Hirokazu 群馬大学, 大学院医学系研究科, 教授 (70291086)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | AAV / 小脳 / グリア / 二重感染 / ATP受容体 |
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| Outline of Final Research Achievements |
Similarly to neurons, glial cells have ATP receptors and may play an active role in neuronal information processing. Originally, in order to examine functional roles of cerebellar glial ATP receptors, I tried to knockdown glial ATP receptors using microRNA-mediated gene silencing technique and it did not work. However, while I tried to express transgenes selectively in cerebellar glial cells with adeno-associated virus (AAV) vectors, I managed to develop a method in which larger transgenes can be expressed in glial cells, compared to the usual usage of AAV vectors. The pros and cons of this method were examined experimentally, and I am preparing for publishing the results.
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