Kinetics of synaptic vesicle filling with neurotransmitter GABA

Research Project

Project/Area Number	25430016
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Neurophysiology / General neuroscience
Research Institution	Doshisha University
Principal Investigator	Hori Tetsuya 同志社大学, 生命医科学部, 准教授 (70396703)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	シナプス小胞 / 前シナプス / GABA / 抑制性シナプス / 小脳 / マウス
Outline of Final Research Achievements	After releasing neurotransmitter, synaptic vesicles are retrieved by endocytosis and recycled vir fast and slow pathways to be reused for synaptic　transmission. To maintai the synaptic efficacy, vesicles must be refilled with neurotransmitter during recycling. We examined the vesicle refilling rate directry at central GABAergic synapses, using caged GABA photolysis. The refilling of vesicles with GABA was about 1 min.

Report

(4 results)