Functional analysis of HLA ortholog expressed on marmoset placenta

• Project/Area Number: 25430095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokai University
• Principal Investigator: KAMETANI Yoshie 東海大学, 医学部, 准教授 (50338787)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Erika 公益財団法人実験動物中央研究所, 部長 (70390739) ; SUZUKI Ryuji 独立行政法人国立病院機構(相模原病院臨床研究センター), 教授 (70373470)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: コモンマーモセット / MHC / 胎盤 / オーソログ / マーモセット / HLA-G / ヒト化マウス / TCRレパートリー / HLAオーソログ / トロフォブラスト / リンパ球 / 妊娠免疫

Outline of Final Research Achievements

The analysis of HLA-G function on pregnant immunity needs primate-based models because of the high evolution speed of MHC molecules and placenta development. We used common marmoset as a non-human primate pregnant model and analyzed the expression of human HLA ortholog and the interaction with maternal immunity. The GFP marmoset placenta developed in the wild type mother enabled to distinguish fetal and maternal tissues. The classical and non-classical marmoset MHC was identified expressed on the trophoblasts from decidua and villi. The secretion type HLA-G ortholog was not detected in the pregnant marmoset peripheral blood-derived plasma.

Research Products

• [Journal Article] 霊長類胎盤の胎児/母体における免疫環境 (2016)
  • Author(s): 木南理仁、亀谷美恵
  • Journal Title: 臨床免疫・アレルギー科 Volume: 65 Pages: 310-315
• [Journal Article] Identification of a CD4 variant in Microminipigs not detectable with available anti-CD4 monoclonal antibodies. (2015)
  • Author(s): Tatsuya Matsubara, Naohito Nishii, Satoshi Takashima, Masaki Takasu, Noriaki Imaeda, Kayo Aiki-Oshimo, Kazuaki Yamazoe, Yoshie Kametani, Asako Ando, Hitoshi Kitagawa
  • Journal Title: Veterinary immunology and Immunopathology Volume: 168 Issue: 3-4 Pages: 176-183
  • DOI: 10.1016/j.vetimm.2015.09.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] B cell Epitope-based Vaccination Therapy (2015)
  • Author(s): Yoshie Kametani *, Asuka Miyamoto, Banri Tsuda, Yutaka Tokuda
  • Journal Title: Antibodies Volume: 4 Issue: 3 Pages: 225-239
  • DOI: 10.3390/antib4030225
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] mproved hematopoietic differentiation of primate embryonic stem cells by inhibition of the PI3K-AKT pathway under defined conditions. (2015)
  • Author(s): Takenobu Nii, Tomotoshi Marumoto, Hiroshi Kohara, Saori Yamaguchi, Hirotaka Kawano, Erika Sasaki, Yoshie Kametani, Kenzaburo Tani
  • Journal Title: Exp Hematol Volume: 43 Issue: 10 Pages: 901-911
  • DOI: 10.1016/j.exphem.2015.06.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Common marmoset CD117-positive hematopoietic cells possess multipotency (2015)
  • Author(s): Shimada S, Nunomura S, Mori S, Suemizu H, Itoh T, Takabayashi S, Okada Y, Yahata T, Shiina T, Katoh H, Suzuki R, Tani K, Ando K, Yagita H, Habu S, Sasaki E, Kametani Y.
  • Journal Title: Int Immune Volume: 27 Issue: 11 Pages: 567-577
  • DOI: 10.1093/intimm/dxv031
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ヒト化NOGマウスにおける 抗体産生 B 細胞サブセット (2015)
  • Author(s): 宮本あすか、亀谷美恵
  • Journal Title: 臨床免疫・アレルギー科 Volume: 63 Pages: 278-283
  • NAID: 40020393406
• [Journal Article] The Effect of Peptide Treatment on the HLA-Binding and Antibody Production in Peripheral Blood Mononuclear Cells Obtained from Japanese Breast Cancer Patients (2015)
  • Author(s): Banri Tsuda*, Yoshie Kametani, Asuka Miyamoto, Hirohito Miyako, Nobue Kumaki, Rin Ogiya, Risa Oshitanai, Mayako Terao, Toru Morioka, Naoki Niikura, Takuho Okamura, Yuki Saito, Yasuhiro Suzuki and Yutaka Tokuda
  • Journal Title: Vaccines and Vaccination Volume: 6 Issue: 01 Pages: 270-270
  • DOI: 10.4172/2157-7560.1000270
  • Peer Reviewed / Open Access
• [Journal Article] Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody (2014)
  • Author(s): Ohshima S, Mori S, Shigenari A, Miyamoto A, Takasu M, Imaeda N, Nunomura S, Okayama Y, Tanaka M, Kitagawa H, Kulski JK, Inoko H, Ando A, Kametani Y.
  • Journal Title: BioScience Trends Volume: 8 Issue: 6 Pages: 308-315
  • DOI: 10.5582/bst.2014.01084
  • NAID: 130005054671
  • ISSN: 1881-7815, 1881-7823
  • Peer Reviewed
• [Journal Article] NKG2D functions as an activating receptor on natural killer cells in common marmoset (callithrix jacchus). (2014)
  • Author(s): Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, Ebara M, Sato T, Nakamura M, Omine K, Kametani Y, Suzuki R, Ogasawara K.
  • Journal Title: Int Immunol. Volume: 26 Issue: 11 Pages: 597-606
  • DOI: 10.1093/intimm/dxu053
  • Peer Reviewed / Open Access
• [Journal Article] Defect of tropomyosin-related kinase B isotype expression in ovarian clear cell adenocarcinoma. (2014)
  • Author(s): Goto Y, Kametani Y*, Kikugawa A, Tsuda B, Miyazawa M, Kajiwara H, Terao Y, Takekoshi S, Nakamura N, Takeda S, Mikami M
  • Journal Title: BioScience Trends Volume: 8 Pages: 93-100
  • NAID: 130005054667
  • Peer Reviewed
• [Journal Article] Genomic sequence analysis of the major histocompatibility complex (MHC) class I G/F segment in common marmoset (Callithrix jacchus). (2014)
  • Author(s): Kono A, Brameier M, Roos C, Suzuki S, Shigenari A, Kametani Y, Kitaura K, Suzuki R, Inoko H, Walter L, Shiina T
  • Journal Title: J. Immunol. Volume: 192 Pages: 3239-3246
  • Peer Reviewed
• [Journal Article] Defect of tropomyosin-related kinase B isotype expression in ovarian clear cell adenocarcinoma. (2014)
  • Author(s): Goto Y et al.
  • Journal Title: BioScience Trends Volume: 未定
  • Peer Reviewed
• [Journal Article] Genomic sequence analysis of the major histocompatibility complex (MHC) class I G/F segment in common marmoset (Callithrix jacchus) (2014)
  • Author(s): Kono A, Brameier M, Roos C, Suzuki S, Shigenari A, Kametani Y, Kitaura K, Matsutani T, Suzuki R, Inoko H, Walter L, Shiina T
  • Journal Title: J Immunol. Volume: 192 Issue: 7 Pages: 3239-3246
  • DOI: 10.4049/jimmunol.1302745
  • Peer Reviewed
• [Journal Article] エストロゲンと胎盤形成；新たな非ヒト霊長類コモンマーモセットを用いたヒト妊娠免疫モデルの提案 (2013)
  • Author(s): 亀谷美恵 他
  • Journal Title: 比較内分泌学 Volume: 40 Pages: 11-13
  • NAID: 130004509413
• [Journal Article] 非ヒト霊長類コモンマーモセット免疫系の重度免疫不全マウスへの移植による再構築 (2013)
  • Author(s): 亀谷美恵 他
  • Journal Title: J.Germfree life and Gnotobiol Volume: 43 Pages: 113-116
  • Peer Reviewed
• [Presentation] PBL-NOG-hIL-4 mouse promotes GVHD-free human humoral immunity (2016)
  • Author(s): Yoshie Kametani, Asuka Miyamoto, Ikumi Katano, Banri Tsuda, Kiyoshi Ando, Yutaka Tokuda, Mamoru Ito, Sonoko Habu
  • Organizer: 5th International Workshop on Humanized Mice
  • Place of Presentation: University of Zurich, Zurich, Switzerland
  • Year and Date: 2016-01-28
  • Int'l Joint Research
• [Presentation] ヒト胎盤における癌遺伝子TrkBと免疫抑制遺伝子PD-L1の共発現 (2015)
  • Author(s): 上絵里奈、木南理仁、寺山隼人、坂部貢、石本人士、三上幹男、亀谷美恵
  • Organizer: 日本生殖免疫学会総会・学術集会
  • Place of Presentation: 熊本県熊本市・くまもと県民交流館パレア
  • Year and Date: 2015-11-21
• [Presentation] Characterization of the monoclonal antibody against SLA-1 allotypes. (2015)
  • Author(s): Ohshima Shino, Ando Asako, Miyamoto Asuka, Nishigami Erina, Kinami Rihito, Kametani Yoshie
  • Organizer: The 44rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Sapporo Convention Center, Sapporo , Hokkaido
  • Year and Date: 2015-11-18
• [Presentation] Characterization of Lymphocyte in Pregnant Immunity (2015)
  • Author(s): Kinami Rihito, Nishigami Erina, Mori Shuya, Suzuki Ryuji, Sasaki Erika, Ito Mamoru, Kametani Yoshie
  • Organizer: The 44rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Sapporo Convention Center, Sapporo , Hokkaido
  • Year and Date: 2015-11-18
• [Presentation] Possible potential of TIGIT for serial transfer of anergy status into activated naïve T cells. (2015)
  • Author(s): Negishi Naoko, Uchida Koichiro, Sato Takehito, Kametani Yoshie, Okumura Ko, Habu Sonoko
  • Organizer: The 44rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Sapporo Convention Center, Sapporo , Hokkaido
  • Year and Date: 2015-11-18
• [Presentation] Common marmoset CD117-positive hematopoietic cells possess multipotency (2015)
  • Author(s): Kametani Yoshie, Shimada Shin, Nunomura Satoshi, Suemizu Hiroshi, Takabayashi Shuji, Shiina Takashi, Katoh Hideki, Suzuki Ryuji, Habu Sonoko, Sasaki Erika
  • Organizer: 44rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Sapporo Convention Center, Sapporo , Hokkaido
  • Year and Date: 2015-11-18
• [Presentation] Antibody-based Her2 peptide CH401MAP as a candidate of breast cancer vaccine. (2015)
  • Author(s): Asuka Miyamoto, Banri Tsuda, Yoshie Kametani, Yutaka Tokuda
  • Organizer: The 74th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya Congress Center, Nagoya, Aichi
  • Year and Date: 2015-10-08
• [Presentation] Analysis of immune cells in the fetal/maternal interface of the primate placenta. (2014)
  • Author(s): Kametani Yoshie, Numao Erina, Mori Shuya, Kinami Rihito, Kitaura Kazutaka, Shiina Takashi, Suzuki Ryuji, Sasaki Erika
  • Organizer: The 43rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] he suppression of naïve T cells are required cell to cell contact by TSST-1 induced anergy T cells. (2014)
  • Author(s): Negishi Naoko, Sato Takehito, Kametani Yoshie, Hori Shohei, Yagita Hideo, Okumura Ko, Habu Sonoko
  • Organizer: The 43rd Annual meeting of the Japanese Society for Immunology.
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Analysis of the humoral immunity of breast cancer patients as the candidates of a new Her2 peptide vaccine CH401MAP. (2014)
  • Author(s): Miyamoto Asuka, Tsuda Banri, Tokuda Yutaka, Kametani Yoshie
  • Organizer: The 43rd Annual meeting of the Japanese Society for Immunology.
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Analysis of the human immunological system reconstructed in the hIL4-Tg NOG mouse. (2014)
  • Author(s): Kikuchi Yusuke, Miyamoto Asuka, Tsuda Banri, Katano Ikumi, Tokuda Yutaka, Ito Mamoru, Kametani Yoshie
  • Organizer: The 43rd Annual meeting of the Japanese Society for Immunology
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Improved Hematopoietic Differentiation of Primate Embryonic Stem Cells (2014)
  • Author(s): Takenobu Nii, Tomotoshi Marumoto, Saori Yamaguchi, Hirotaka Kawano, Yoshie Kametani, , and Kenzaburo Tani
  • Organizer: 56th AHS Annual meeting and exposition
  • Place of Presentation: San Francisco, CA
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] NOGマウス環境下で分化するtransitional B細胞の抗体産生細胞の解析 (2014)
  • Author(s): 小島美香、森 修弥、嶋田 新、大島 志乃、伊藤 守、鈴木 隆二、安藤 潔、亀谷 美恵
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 超小型実験動物用ブタ：マイクロミニピッグにおけるCD4多型の検出と免疫機能 (2014)
  • Author(s): 松原達也、安藤麻子、高須正規、西飯直仁、今枝紀明、高島 諭、秋岡幸兵、西村崇史、亀谷美恵、北川 均
  • Organizer: 日本動物遺伝育種学会第15回大会
  • Place of Presentation: 和光（理研）
  • Year and Date: 2014-10-30 – 2014-11-01
• [Presentation] 超小型実験動物用ブタ：マイクロミニピッグにおけるCD4多型 (2014)
  • Author(s): 松原達也、〇安藤麻子、高須正規、西飯直人、今枝紀明、亀谷美恵、北川 均
  • Organizer: 23回日本組織適合性学会大会
  • Place of Presentation: 長崎市（長崎大学 医学部）
  • Year and Date: 2014-09-13 – 2014-09-15
• [Presentation] CD4 polymorphism in Microminipigs (2014)
  • Author(s): Tatsuya MATSUBARA, Masaki TAKASU, Naohito NISHII, Noriaki IMAEDA, Asako ANDO, Yoshie KAMETANI, Jerzy K. KULSKI, Hitoshi KITAGAWA
  • Organizer: 34th International Society for Animal Genetics Conference
  • Place of Presentation: 西安 (Qujiang International Conference Centre, Xi’an, China)
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] コモンマーモセット胎盤における妊娠免疫系の解析 (2014)
  • Author(s): 森修弥、沼尾絵里奈、大島志乃、嶋田新、岡原純子、佐々木えりか、鈴木隆二、石本人士、椎名隆、亀谷美恵
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌・札幌コンベンションセンター
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] ヒト免疫系再構築マウスTransitional B細胞の抗体産生能解析 (2014)
  • Author(s): 小島美香、森修弥、大島志乃、伊藤守、安藤潔、亀谷美恵
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌・札幌コンベンションセンター
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] c-kit発現細胞の造血能に関する比較生物学的解析 (2014)
  • Author(s): 大島志乃、嶋田新、森修弥、小島美香、宮本あすか、椎名隆、安藤麻子、亀谷美恵
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌・札幌コンベンションセンター
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] コモンマーモセットMHC遺伝子に置けるDNAタイピング法の開発と多型解析 (2014)
  • Author(s): 椎名隆、重成敦子、森修弥、北浦一孝、亀谷美恵、鈴木隆二
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌・札幌コンベンションセンター
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] A new anti-HER2 peptide "CH401MAP" can stimulate immunity in breast cancer patients (2013)
  • Author(s): 津田万里他
  • Organizer: 36th San Antonio Breast Cancer symposium
  • Place of Presentation: San Antonio, Texas, USA
• [Presentation] Antibody secreting B cell subsets developed in humanized NOG mouse
  • Author(s): Yoshie Kametani et al.
  • Organizer: 第42回日本免疫学会・学術集会
  • Place of Presentation: 千葉・幕張メッセ
• [Presentation] 卵巣名細胞がんにおいて多様なTrkBアイソフォームの発現が亢進している
  • Author(s): 後藤優美子他
  • Organizer: 第72回日本癌学会総会
  • Place of Presentation: 神奈川・パシフィコ横浜
• [Presentation] HER2部分配列を持つCH401MAP peptideの乳がん患者T細胞/B細胞 相互作用の解析
  • Author(s): 津田万里他
  • Organizer: 第21回日本乳癌学会学術総会
  • Place of Presentation: 静岡・アクトシティ浜松
• [Presentation] コモンマーモセットにおけるリアルタイムPCRを用いた免疫関連遺伝子発現解析
  • Author(s): 北浦一孝他
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 茨城・筑波国際会議場
• [Presentation] ブタc-kitに対するモノクローナル抗体作製と性状解析
  • Author(s): 大島志乃他
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 茨城・筑波国際会議場
• [Presentation] ヒト免疫系再構築NOGマウスB細胞の性状解析
  • Author(s): 森修弥他
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 茨城・筑波国際会議場
• [Patent(Industrial Property Rights)] ブタ主要組織適合性抗原(MHC) SLA-1を特異的に認識するモノクローナル抗体 (2015)
  • Inventor(s): 亀谷美恵・安藤麻子・大島志乃・平山令明
  • Industrial Property Rights Holder: 亀谷美恵・安藤麻子・大島志乃・平山令明
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-004905
  • Filing Date: 2015-01-14