| Project/Area Number |
25430098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
KAWAI KENJI 公益財団法人実験動物中央研究所, その他部局等, 研究員 (30414064)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Masato 東海大学, 医学部, 教授 (00164335)
宮城 洋平 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, その他 (00254194)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAGI Yohei 神奈川県立がんセンター, 臨床研究所, 部長 (00254194)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 疾患モデル / 個別化医療 / NOGマウス / NOG |
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| Outline of Final Research Achievements |
We established rapid and effective protocols for making individual Cancer Xenograft (iCX). In the iCXs, morphological characteristics, gene expression profiles, and genetic alteration patterns were all well preserved. In colorectal cancer iCXs, Collagen gel droplet embedded culture-drug sensitivity tests (CD-DST) were performed. The CD-DST results between original and iCX specimens were generally correlated (R2=0.01-0.89). The result of CD-DST using iCX was compatible with clinical effects of anti-cancer drug. Zinc finger protein 185 (ZNF185) is one of proteins selected from proteome analysis utilizing cancer xenograft models. We also analyzed the association between ZNF185 and the clinicopathological characteristics of colon cancer. ZNF185 expression was found to be an independent indicator of liver metastasis and prognosis in patients with colon cancer. Our iCXs with clinical information are a promising advance that may accelerate the development of anticancer therapies.
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