The role of UBR4/HPV16 E7 interaction in cancer
| Project/Area Number |
25430119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
TASAKI Takafumi 金沢医科大学, 総合医学研究所, 准教授 (70629815)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | パピローマウイルス / がん / E7 / UBR4 / 代謝的安定性 / N-end rule pathway / UBR box domain / HPV16 E7 / がん化 / 子宮頸がん / HPVE7 / UBR box / UBR box protein / Ubiquitin / Protein regulation / HPV16E7 / cancer |
|---|
| Outline of Final Research Achievements |
Cervical cancer is caused by certain types of human papilloma virus. The viral E7 is a small 98-amino acid protein that binds various cellular proteins in a host cell. Among these interaction proteins, UBR4 is a huge approx. 5800-amino acids protein. This study found out that the E7 binds to UBR4 via the conservative UBR box domain in UBR4. The UBR box domain is involved in a physiological role of UBR4. Next, we investigated a metabolic stability of the E7 in genetically modified UBR4-deficient cells. The E7 was significantly unstable in the UBR4-lacking cells. Our results suggest that the E7 forms a stable complex with the UBR4 and the complex regulates the viral cancer activity in host cells.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] UBR box N-recognin-4 (UBR4), an N-recognin of the N-end rule pathway, and its role in yolk sac vascular development and autophagy2013
Author(s)
Tasaki T, Kim ST, Zakrzewska A, Lee BE, Kang MJ, Yoo YD, Cha-Molstad HJ, Hwang J, Soung NK, Sung KS, Kim SH, Nguyen MD, Sun M, Yi EC, Kim BY, Kwon YT
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Journal Title
Proc Natl Acad Sci USA
Volume: 110
Issue: 10
Pages: 3800-3805
DOI
Related Report
Peer Reviewed
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[Journal Article] The N-end rule proteolytic system in autophagy2013
Author(s)
Kim ST, Tasaki T, Zakrzewska A, Yoo YD, Sa Sung K, Kim SH, Cha-Molstad H, Hwang J, Kim KA, Kim BY, Kwon YT
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Journal Title
Autophagy
Volume: 9
Issue: 7
Pages: 1100-1103
DOI
Related Report
Peer Reviewed
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