| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Outline of Final Research Achievements |
Cervical cancer is caused by certain types of human papilloma virus. The viral E7 is a small 98-amino acid protein that binds various cellular proteins in a host cell. Among these interaction proteins, UBR4 is a huge approx. 5800-amino acids protein. This study found out that the E7 binds to UBR4 via the conservative UBR box domain in UBR4. The UBR box domain is involved in a physiological role of UBR4. Next, we investigated a metabolic stability of the E7 in genetically modified UBR4-deficient cells. The E7 was significantly unstable in the UBR4-lacking cells. Our results suggest that the E7 forms a stable complex with the UBR4 and the complex regulates the viral cancer activity in host cells.
|
