Development of novel tumor markers for renal cell carcinoma by evaluating small nuclear acid in blood

• Project/Area Number: 25430137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Kagoshima University
• Principal Investigator: tatarano shuichi 鹿児島大学, 医歯学域医学系, 助教 (30624655)
• Co-Investigator(Kenkyū-buntansha): KOJIMA SATOKO 帝京大学, 医学部, 准教授 (10345019) ; SEKI NAOHIKO 千葉大学, 大学院医学研究院, 准教授 (50345013) ; ENOKIDA HIDEKI 鹿児島大学, 医歯学域医学系, 准教授 (80347103) ; NAKAGAWA MASAYUKI 鹿児島大学, 医歯学域医学系, 教授 (90164144)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: マイクロRNA / 腎細胞癌 / 腫瘍マーカー / microRNA

Outline of Final Research Achievements

We focused on miR-200s (miR-200a/miR-200b/miR-429 cluster and miR-200c/miR-141cluster). ZEB1/2 genes that are key molecules for EMT were repressed by miR-200s transfection. miR-138 targeted Vimentin which was under stream of ZEB1/2. Tumor suppressive miR-29s repressed LOXL2 and lead to instability of ECM. These microRNAs could be a biomarker of renal cell carcinoma.

Research Products

• [Journal Article] Expression of the tumor suppressive miRNA-23b/27b cluster is a good prognostic marker in clear cell renal cell carcinoma. (2014)
  • Author(s): Ishihara T, Seki N, Inoguchi S, Yoshino H, Tatarano S, Yamada Y, Itesako T, Goto Y, Nishikawa R, Nakagawa M, Enokida H.
  • Journal Title: The Journal of Urology Volume: 192 Issue: 6 Pages: 1822-1830
  • DOI: 10.1016/j.juro.2014.07.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tumor-suppressive microRNA-143/145 cluster targets hexokinase-2 in renal cell carcinoma. (2013)
  • Author(s): Yoshino H, Enokida H, Itesako T, Kojima S, Kinoshita T, Tatarano S, Chiyomaru T, Nakagawa M, Seki N.
  • Journal Title: Cancer Science Volume: 104 Pages: 1567-1574
  • Peer Reviewed
• [Journal Article] Tumor-suppressive microRNA-1291 directly regulates glucose transporter 1 in renal cell carcinoma. (2013)
  • Author(s): Yamasaki T, Seki N, Yoshino H, Itesako T, Yamada Y, Tatarano S, Hidaka H, Yonezawa T, Nakagawa M, Enokida H.
  • Journal Title: Cancer Science Volume: 104 Pages: 1411-1419
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition-related microRNA-200s regulate molecular targets and pathways in renal cell carcinoma. (2013)
  • Author(s): Yoshino H, Enokida H, Itesako T, Tatarano S, Kinoshita T, Fuse M, Kojima S, Nakagawa M, Seki N.
  • Journal Title: Journal of Human Genetics Volume: 58 Pages: 1059-1068
  • NAID: 10031195279
  • Peer Reviewed
• [Journal Article] MicroRNA-218 inhibits cell migration and invasion in renal cell carcinoma through targeting caveolin-2 involved in focal adhesion pathway. (2013)
  • Author(s): Yamasaki T, Seki N, Yoshino H, Itesako T, Hidaka H, Yamada Y, Tatarano S, Yonezawa T, Kinoshita T, Nakagawa M, Enokida H.
  • Journal Title: Journal of Urology Volume: 190 Pages: 1059-2068
  • Peer Reviewed
• [Journal Article] Tumor-suppressive microRNA-135a inhibits cancer cell proliferation by targeting the c-MYC oncogene in renal cell carcinoma. (2013)
  • Author(s): Yamada Y, Hidaka H, Seki N, Yoshino H, Yamasaki T, Itesako T, Nakagawa M, Enokida H.
  • Journal Title: Cancer Science Volume: 104 Pages: 304-312
  • Peer Reviewed
• [Presentation] The clustered microRNA-23b/27b function as tumor suppressors and useful prognostic markers in renal cell carcinoma (2014)
  • Author(s): Tomoaki Ishihara
  • Organizer: 2014 AUA annual meeting
  • Place of Presentation: Orland, USA
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] Tumor-suppressive miR-135a inhibits cancer cell proliferation by targeting the c-MYC oncogene in renal cell carcinoma
  • Author(s): Shuichi Tatarano
  • Organizer: American Urological Association Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Tumor suppressive microRNA-218 regulates oncogenes of focal adhesion pathways in renal cell carcinoma
  • Author(s): Takeshi Yamasaki
  • Organizer: American Urological Association Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Tumor suppressive microRNA-138 contributes to inhibition of both cell migration and invasion through targeting vimentin
  • Author(s): Takeshi Yamasaki
  • Organizer: American Urological Association Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Identification of EMT-related miRNA-200 family regulated novel cancer pathways in renal cell carcinoma
  • Author(s): Hirofumi Yoshino
  • Organizer: American Urological Association Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Remarks] 千葉大学大学院医学研究院 先端医学応用講座 機能ゲノム学
  • URL: http://genomejet.jp/research/