Augmentation of tumor immune response by depletion of regulatory T cells

• Project/Area Number: 25430161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Kawasaki University of Medical Welfare
• Principal Investigator: NAKAYAMA EIICHI 川崎医療福祉大学, 医療福祉学部, 教授 (60180428)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん免疫 / 腫瘍内浸潤リンパ球 / 制御性T細胞 / ケモカイン受容体CCR4 / ケモカインCCL22/MDC / 抗体依存性細胞障害性(ADCC) / ヒト化抗CCR4単クローン抗体 / 制御性T細胞除去 / ADCC活性 / 遊走 / 肺癌 / 肺がん / ケモカイン受容体

Outline of Final Research Achievements

Augmentation of tumor immune response by depletion of regulatory T cells (Tregs) was pursued by the following studies. 1. On three Treg subpopulations, resting Tregs, activated Tregs and nonTregs, difference of the expression of CCR4 chemokine receptor on their surface and of their distribution in PBMC and TIL was demonstrated. 2. The presence of CCR4-expressing Tregs and CCL22/MDC in the local tumor site was shown by immunohistochemistry. 3. CD25 CD4 Tregs with migratory activity were shown to be eliminated by CCR4（KM2760）mAb with antibody-dependent cell-mediated cytotoxicity（ADCC）. 4. Efficient depletion of Tregs by injection of humanized anti-CCR4（ KW-0761）mAb in solid cancer patients was demonstrated.

Research Products

• [Journal Article] Immune Responses to the Cancer Testis Antigen XAGE-1b in Non Small Cell Lung Cancer Caucasian Patients. (2016)
  • Author(s): Saito K, Nakayama E, Valmori D.
  • Journal Title: PLOS ONE Volume: 11(3) Issue: 3 Pages: e0150623-e0150623
  • DOI: 10.1371/journal.pone.0150623
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] モガムリズマブの新しい展開 (2016)
  • Author(s): 鈴木 進、中山睿一、黒瀬浩史、上田龍三
  • Journal Title: 最新医学 Volume: 71(1) Pages: 126-133
  • NAID: 40020708354
• [Journal Article] 2015 Guidance on cancer immunotherapy development in early-phase clinical studies. (2015)
  • Author(s): Kageyama S, et al. （他 50名）
  • Journal Title: Cancer Sci. Volume: 106(12) Pages: 1761-1771
  • Peer Reviewed / Open Access
• [Journal Article] Sensitive multiplexed quantitative analysis of autoantibodies to cancer antigens with chemically S-cationized full-length and water-soluble denatured proteins. (2015)
  • Author(s): Futami J, Nonomura H, Kido M, Niidoi N, Fujieda N, Hosoi A, Fujita K, Mandai K, Atago Y, Kinoshita R, Honjo T, Matsushita H, Uenaka A, Nakayama E, and Kakimi K.
  • Journal Title: Bioconjugate Chem. Volume: 26(10) Issue: 10 Pages: 2076-2084
  • DOI: 10.1021/acs.bioconjchem.5b00328
  • Peer Reviewed
• [Journal Article] Contrasting effects of the cytotoxic anticancer drug Gemcitabine and the EGFR tyrosine kinase inhibitor Gefitinib on NK cell-mediated cytotoxicity via regulation of NKG2D ligand in non-small-cell lung cancer cells. (2015)
  • Author(s): Okita R, Wolf D, Yasuda K, Maeda A, Yukawa T, Saisho S, Shimizu K, Yamaguchi Y, Oka M, Nakayama E, Lundqvist A, Kiessling R, Seliger B, Nakata M.
  • Journal Title: PLoS One Volume: 10 Issue: 10 Pages: e0139809-e0139809
  • DOI: 10.1371/journal.pone.0139809
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phase Ia Study of FoxP3+ CD4 Treg Depletion by Infusion of a Humanized Anti-CCR4 Antibody, KW-0761, in Cancer Patients. (2015)
  • Author(s): 2.Kurose K, Ohue Y, Wada H, Iida S, Ishida T, Kojima T, Doi T, Suzuki S, Isobe M, Funakoshi T, Kakimi K, Nishikawa H, Udono H, Oka M, Ueda R, Nakayama E.
  • Journal Title: Clin Cancer Res. Volume: 21 Issue: 19 Pages: 4327-36
  • DOI: 10.1158/1078-0432.ccr-15-0357
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient. (2015)
  • Author(s): Miyai M, Eikawa S, Hosoi A, Iino T, Matsushita H, Isobe M, Uenaka A, Udono H, Nakajima J, Nakayama E, Kakimi K.
  • Journal Title: PLoS One Volume: 10(8) Issue: 8 Pages: e0136086-e0136086
  • DOI: 10.1371/journal.pone.0136086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] がん抗原（第7章）The Frontiers in Life Sciences「がんと免疫」 (2015)
  • Author(s): 中山睿一
  • Journal Title: がん免疫療法のメカニズム解明と臨床への展開 Volume: 1 Pages: 51-59
• [Journal Article] Immune-mediated anti-tumor effect by type 2 diabetes drug, metformin. (2015)
  • Author(s): Eikawa S, Nishida M, Mizukami S, Yamazaki C, Nakayama E and Udono H.
  • Journal Title: Pros Natl Acd Sci USA Volume: 112 (6) Issue: 6 Pages: 1809-1814
  • DOI: 10.1073/pnas.1417636112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increase of activated Treg in TIL in lung cancer and in vitro depletion of Treg by ADCC using an anti-human CCR4 mAb (KM2760). (2015)
  • Author(s): Koji Kurose, Yoshihiro Ohue, Eiichi Sato Akira Yamauchi, Shingo Eikawa, Midori Isobe, Yumi Nishio, Akiko Uenaka, Mikio Oka, and Eiichi Nakayama.
  • Journal Title: J Thorac Oncol. Volume: 10 Issue: 1 Pages: 74-83
  • DOI: 10.1097/jto.0000000000000364
  • Peer Reviewed / Open Access
• [Journal Article] 基礎：抗CCR4抗体の制御性T細胞への影響と臨床応用の可能性 (2015)
  • Author(s): 黒瀬浩史、岡 三喜男、中山睿一
  • Journal Title: 最新医学 Volume: 70 (3) Pages: 393-398
• [Journal Article] がん精巣抗原に対する免疫応答とその臨床的意義 (2015)
  • Author(s): 岡 三喜男、大植祥弘、黒瀬浩史、中山睿一
  • Journal Title: BIO Clinica Volume: 30 (3) Pages: 31-35
• [Journal Article] Prolongation of overall survival in advanced lung adenocarcinoma patients with the XAGE1 (GAGED2a) antibody. (2014)
  • Author(s): Ohue Y, Kurose K, Mizote Y, Matsumoto H, Nishio Y, Isobe M, Fukuda M, Uenaka A, Oka M and Nakayama E.
  • Journal Title: Clin. Cancer Res. Volume: 20 (19) Issue: 19 Pages: 5052-5063
  • DOI: 10.1158/1078-0432.ccr-14-0742
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Antibody response to cancer/testis (CT) antigens: A prognostic marker in cancer patients. (2014)
  • Author(s): Ohue Y, Wada H, Oka M and Nakayama E.
  • Journal Title: OncoImmunology Volume: 3 Pages: 11-11
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic variants of immunoglobulin γ and κ chains influence humoral immunity to the cancer-testis antigen XAGE-1b (GAGED2a) in patients with non-small cell lung cancer. (2014)
  • Author(s): Pandey JP, Namboodiri AM, Ohue Y, Oka M, Nakayama E.
  • Journal Title: Clinical Experimental Immunology Volume: 176 Issue: 1 Pages: 78-83
  • DOI: 10.1111/cei.12247
  • Peer Reviewed
• [Journal Article] Vaccination With NY-ESO-1 Overlapping Peptides Mixed With Picibanil OK-432 and Montanide ISA-51 in Patients With Cancers Expressing the NY-ESO-1 Antigen (2014)
  • Author(s): Wada H, Isobe M, Kakimi K, Mizote Y, Eikawa S, Sato E, Takigawa N, Kiura K, Tsuji K, Iwatsuki K, Yamasaki M, Miyata H, Matsushita H, Udono H, Seto Y, Yamada K, Nishikawa H, Pan L, Venhaus R, Oka M, Doki Y, Nakayama E.
  • Journal Title: J Immunother Volume: 37(2) Issue: 2 Pages: 84-92
  • DOI: 10.1097/cji.0000000000000017
  • Peer Reviewed
• [Journal Article] Production of NY-ESO-1 peptide/DRB1*08:03 tetramers and ex vivo detection of CD4 T-cell responses in vaccinated cancer patients. (2014)
  • Author(s): Mizote Y, Uenaka A, Isobe M, Wada H, Kakimi K, Saika T, Kita S, Koide Y, Oka M, Nakayama E.
  • Journal Title: Vaccine. Volume: Epub 2014 Jan 5. Issue: 8 Pages: 957-964
  • DOI: 10.1016/j.vaccine.2013.12.042
  • Peer Reviewed
• [Journal Article] TLR4 and NLRP3 inflammasome activation in monocytes by N-propionyl cysteaminylphenol-maleimide-dextran (NPCMD). (2014)
  • Author(s): Mizote, Y., Wakamatsu, K., Ito, S., Uenaka, A., Ohue, Y., Kurose, K., Isobe, M., Ito, A., Tamura, Y., Honda, H., Yamashita, T., Nohara, S., Oka, M., Jimbow, K., Nakayama, E.
  • Journal Title: J. Dermatol. Sci. Volume: 73 Issue: 3 Pages: 76-87
  • DOI: 10.1016/j.jdermsci.2013.11.006
  • Peer Reviewed
• [Journal Article] Melanoma-targeted chemo-thermotherapy and in situ peptide immunotherapy through HSP production by using melanogenesis substrate, NPrCAP and magnetite nanoparticles. (2013)
  • Author(s): Jimbow, K.
  • Journal Title: J. Skin Cancer Volume: 2013 Pages: 174-180
  • DOI: 10.1155/2013/742925
  • Peer Reviewed
• [Journal Article] Establishment of animal models to analyze the kinetics and distribution of human tumor antigen-specific CD8+ T cells (2013)
  • Author(s): Muraoka D, Nishikawa H, Noguchi T, Wang L, Harada N, Sato E, Luescher I, Nakayama E, Kato T, Shiku H
  • Journal Title: Vaccine Volume: 31(17) Issue: 17 Pages: 2110-2118
  • DOI: 10.1016/j.vaccine.2013.02.056
  • Peer Reviewed
• [Journal Article] Prostate cancer progression correlates with increased humoral immune response to a human endogenous retrovirus GAG protein. (2013)
  • Author(s): Reis BS, Jungbluth AA, Frosina D, Holz M, Ritter E, Nakayama E, Ishida T, Obata Y, Carver B, Scher H, Scardino P, Slovin S, Subudhi SK, Reuter VE, Savage C, Allison JP, Melamed JM, Jager E, Ritter G, Old LJ, Gnjatic S.
  • Journal Title: Clin. Cancer Res. Volume: 19(22) Issue: 22 Pages: 6112-6125
  • DOI: 10.1158/1078-0432.ccr-12-3580
  • Peer Reviewed
• [Journal Article] Which patients respond best to hepatitis B vaccination after a hepatitis B virus-related liver transplantation? (2013)
  • Author(s): Takaki A, Yagi T, Yasunaka T, Sadamori H, Shinoura S, Umeda Y, Yoshida R, Sato D, Nobuoka D, Utsumi M, Yasuda Y, Nakayama E, Miyake Y, Ikeda F, Shiraha H, Nouso K, Fujiwara T, Yamamoto K
  • Journal Title: J Gastroenterol Volume: 48(12) Issue: 12 Pages: 1373-1383
  • DOI: 10.1007/s00535-013-0763-8
  • Peer Reviewed
• [Journal Article] がん患者における免疫モニタリング―評価法と国際標準化 (2013)
  • Author(s): 和田 尚、中山睿一
  • Journal Title: 週刊 医学のあゆみ がんの免疫制御―研究と臨床の最前線 Volume: 244(9) Pages: 871-877
• [Journal Article] 免疫バイオマーカーと抗腫瘍効果 (2013)
  • Author(s): 和田 尚、中山睿一
  • Journal Title: 実験医学 増刊 腫瘍免疫学とがん免疫療法 Volume: 31(12) Pages: 204-208
• [Journal Article] T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles (2012)
  • Author(s): Ito A, Yamaguchi M, Okamoto N, Sanematsu Y,Kawabe Y, Wakamatsu K, Ito S, Honda H, Kobayashi T, Nakayama E, Tamura Y, Okura M, Yamashita T, Jimbow K, Kamihara M
  • Journal Title: Nanomedicine Volume: (epub ahead of print) Issue: 6 Pages: 891-902
  • DOI: 10.2217/nnm.12.142
  • Peer Reviewed
• [Presentation] Phase Ⅰa study of FoxP3+ CD4 Treg depletion by infusion of anti-CCR4 antibody in cancer patients (2015)
  • Author(s): 黒瀬浩史, 大植祥弘, 西尾祐美, 磯辺みどり, 上中明子, 中山睿一, 岡三喜男
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2015-11-26
• [Presentation] 腫瘍PD-L1, Gal-9, XAGE1発現及びT細胞浸潤を指標とした肺腺癌の予後予測 (2015)
  • Author(s): 大植祥弘, 黒瀬浩史, 西尾祐美, 磯辺みどり, 上中明子, 中山睿一, 岡三喜男
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2015-11-26
• [Presentation] Phase I a study of FoxP3+ CD4 Treg depletion by infusion of a humanized anti-CCR4 antibody, KW-0761, in cancer patients (2015)
  • Author(s): 黒瀬浩史, 大植祥弘, 和田尚, 飯田真介, 石田高司, 鈴木進, 垣見和宏, 西川博嘉, 上田龍三, 岡三喜男, 中山睿一
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] Augmentation of anti-tumor immune responses by N-propionyl cysteaminylphenol-maleimid-dextran (NPCMD) (2015)
  • Author(s): 西尾祐美, 溝手雄, 上中明子, 黒瀬浩史, 大植祥弘, 野原聡, 岡三喜男, 中山睿一
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] Survival prediciting discriminant function with immune parameters for lung adenocarcinoma patients (2015)
  • Author(s): 大植祥弘, 黒瀬浩史, 西尾祐美, 磯辺みどり, 上中明子, 岡三喜男, 中山睿一
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] Immunotherapy for solid tumors based on regulation of regulatory T lymphocyte (2015)
  • Author(s): 鈴木進, 石田高司, 黒瀬浩史, 大植祥弘, 西川博嘉, 飯田真介, 和田尚, 岡三喜男, 中山睿一, 上田龍三
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] The Inaugural International Cancer Immunotherapy Conference、New York (2015)
  • Author(s): Ohue Y, Kurose K, Oka M, Nakayama E
  • Organizer: The Inaugural International Cancer Immunotherapy Conference
  • Place of Presentation: Sheraton New York Times Square Hotel （New York, USA）
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] Augmentation of anti-tumor immune responses by N-propionyl cysteaminylphenol-maleimid-dextran (NPCMD) (2015)
  • Author(s): Yumi Nishio, Yu Mizote, Akiko Uenaka, Koji Kurose, Yoshihiro Ohue, Satoshi Nohara, Mikio Oka, Kowichi Jimbow, Eiichi Nakayama
  • Organizer: 第19回日本がん免疫学会総会
  • Place of Presentation: 伊藤国際学術研究センター 伊藤謝恩ホール（東京）
  • Year and Date: 2015-07-09
• [Presentation] 非小細胞肺がん患者で、免疫グロブリンの遺伝的変異がXAGE1免疫に及ぼす影響 (2015)
  • Author(s): 堅田洋佑, 大植祥弘, 黒瀬浩史, 福田実, 上中明子, JP Pandey, 中山睿一, 岡三喜男
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京）
  • Year and Date: 2015-04-17
• [Presentation] 肺癌の腫瘍浸潤リンパ球（TIL）における活性化・抑制性分子の解析 (2015)
  • Author(s): 黒瀬浩史, 大植祥弘, 西尾祐美, 磯辺みどり, 上中明子, 中山睿一, 岡三喜男
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京）
  • Year and Date: 2015-04-17
• [Presentation] Clinical effect of XAGE1 (GAGED2a) antigen expression and antibody response in EGFR mutated and wild-type advanced lung adenocarcinoma (2015)
  • Author(s): Yoshihiro Ohue, Koji Kurose, Yu Mizote, Hirofumi Matsumoto, Yumi Nishio, Midori Isobe, Minoru Fukuda, Akiko Uenaka, Eiichi Nakayama, Mikio Oka
  • Organizer: 55th Annual Meeting of The Japanese Respiratory Society
  • Place of Presentation: Tokyo Internatuinal Forum (Tokyo)
  • Year and Date: 2015-04-17
• [Presentation] 肺腺癌でXAGE1に対する宿主免疫応答の解析と予後延長効果の証明 (2015)
  • Author(s): 大植祥弘, 黒瀬浩史, 磯辺みどり, 上中明子, 福田実, 中山睿一, 岡三喜男
  • Organizer: 第112回日本内科学会講演会
  • Place of Presentation: みやこめっせ（京都）
  • Year and Date: 2015-04-10
• [Presentation] 進行期肺腺癌におけるXAGE1（GAGED2a）免疫と予後 (2015)
  • Author(s): 大植祥弘, 黒瀬浩史, 岡 三喜男, 中山睿一
  • Organizer: 第12回日本免疫治療学研究会学術集会
  • Place of Presentation: 東京ガーデンパレス（東京都文京区）
  • Year and Date: 2015-02-28
  • Invited
• [Presentation] Clinical effect of XAGE1(GAGED2a) immunity in advanced lung adenocarcinoma. (2015)
  • Author(s): 大植祥弘, 黒瀬浩史, 岡 三喜男, 中山睿一
  • Organizer: 第12回日本免疫治療学研究会学術集会
  • Place of Presentation: 東京ガーデンパレス（東京都文京区）
  • Year and Date: 2015-02-28
• [Presentation] 非小細胞肺がん患者で、免疫グロブリンγ鎖およびκ鎖の遺伝的変異がXAGE1免疫に及ぼす影響 (2014)
  • Author(s): 大植祥弘, Janardan Pandey, Aryan Namboodiri, 中山睿一, 岡 三喜男
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-11-14
• [Presentation] 非小細胞肺癌におけるXAGE1免疫の予後延長効果 (2014)
  • Author(s): 大植祥弘, 黒瀬浩史, 溝手 雄, 松本博文, 西尾祐美, 磯辺みどり, 上中明子, 福田 実, 中山睿一, 岡 三喜男
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-11-14
• [Presentation] Increase of activated Tregs in TIL in lung cancer and depletion of Tregs using anti-human CCR4 mAb. (2014)
  • Author(s): 黒瀬浩史, 大植祥弘, 西尾祐美, 磯辺みどり, 堅田洋佑, 阿部公亮, 上中明子, 中山睿一, 岡 三喜男
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-11-14
• [Presentation] Clinical effect of XAGE1 (GAGED2a) antigen expression and antibody response in EGFR mutated and wild-type advanced lung adenocarcinoma. (2014)
  • Author(s): Yoshihiro Ohue, Koji Kurose, Yu Mizote, Hirofumi Matsumoto, Yumi Nishio, Midori Isobe, Minoru Fukuda, Akiko Uenaka, Mikio Oka, and Eiichi Nakayama.
  • Organizer: 22nd Annual International Cancer Immunotherapy Symposium
  • Place of Presentation: New York （USA）
  • Year and Date: 2014-10-05
• [Presentation] Increase of activated Tregs in TIL in lung cancer and in vitro depletion of Tregs by ADCC using anti-human CCR4 mAb (KM2760). (2014)
  • Author(s): Koji Kurose, Yoshihiro Ohue, Shingo Eikawa, Yumi Nishio, Midori Isobe, Akiko Uenaka, Mikio Oka, and Eiichi Nakayama.
  • Organizer: 22nd Annual International Cancer Immunotherapy Symposium
  • Place of Presentation: New York （USA）
  • Year and Date: 2014-10-05
• [Presentation] Increase of activated Tregs in TIL in Lung cancer and in vitro depletion of Tregs by ADCC using anti-human CCR4 mAb (2014)
  • Author(s): Koji Kurose, Yoshihiro Ohue, Shingo Eikawa, Yumi Nishio, Midori Isobe, Akiko Uenaka, Mikio Oka, Eiichi Nakayama
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-27
• [Presentation] Clinical effect of XAGE1 immune response in EGFR mutated and wild-type advanced lung adenocarcinoma (2014)
  • Author(s): Yoshihiro Ohue, Koji Kurose, Hirofumi Matsumoto, Yu Mizote, Yumi Nishio, Midori Isobe, Minoru Fukuda, Akiko Uenaka, Mikio Oka, Eiichi Nakayama
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25
• [Presentation] Targeted immunotherapy strategy for non-small cell lung cancer (2014)
  • Author(s): Mikio Oka, Koji Kurose, Yoshihiro Ohue, Eiichi Nakayama
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25
  • Invited
• [Presentation] Monitoring multifunctionality of exhausted CD8 T-cells in cancer patients (2014)
  • Author(s): Mami Negawa, Shingo Eikawa, Takenori Uehara, Yuki Kunisada, Yoshihiro Ohue, Koji Kurose, Midori Isobe, Akiko Uenaka, Eiichi Nakayama, Heiichiro Udono
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25
• [Presentation] Increase of activated Tregs in TIL in lung cancer and in vitro depletion of Tregs by ADCC using anti-human CCR4 mAb (KM2760) (2014)
  • Author(s): 黒瀬浩史、大植祥弘、榮川伸吾、西尾祐美、磯辺みどり、上中明子、岡 三喜男、中山睿一
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: ひめぎんホール（愛媛県松山市）
  • Year and Date: 2014-07-31
• [Presentation] 制御性T細胞解析方法の標準化に向けた多施設共同研究 (2014)
  • Author(s): 長瀬博次、和田 尚、西川博嘉、鈴木 進、平家勇司、小嶋隆嗣、垣見和宏、舩越 健、飯田真介、石田高司、佐藤永一、鵜殿平一郎、岡 三喜男、中山睿一、土岐祐一郎、上田龍三
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: ひめぎんホール（愛媛県松山市）
  • Year and Date: 2014-07-31
• [Presentation] Clinical effect of XAGE-1 (GAGED2a) antigen expression and antibody response in EGFR mutated and wild-type advanced lung adenocarcinoma. (2014)
  • Author(s): Yoshihiro Ohue, Koji Kurose, Yu Mizote, Hirofumi Matsumoto, Yumi Nishio, Midori Isobe, Minoru Fukuda, Akiko Uenaka, Mikio Oka, Eiichi Nakayama
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: ひめぎんホール（愛媛県松山市）
  • Year and Date: 2014-07-30
• [Presentation] TCRディープシーケンスによるNY-ESO-1特異的T細胞のモニタリング (2014)
  • Author(s): 垣見和宏、榮川伸吾、磯辺みどり、松下博和、宮井まなみ、細井亮宏、藤枝奈緒、鵜殿平一郎、上中明子、中山睿一
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: ひめぎんホール（愛媛県松山市）
  • Year and Date: 2014-07-30
• [Presentation] Prolonged overall survival in XAGE-1b (GAGED2a) antibody positive NSCLC patients. (2014)
  • Author(s): 大植祥弘、黒瀬浩史、溝手 雄、松本博文、磯辺みどり、上中明子、福田 実、中山睿一、岡 三喜男
  • Organizer: 第54回日本呼吸器学会学術講演会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2014-04-25
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2014)
  • Author(s): 黒瀬浩史、大植祥弘、西尾祐美、磯辺みどり、上中明子、岡 三喜男、中山睿一
  • Organizer: 第54回日本呼吸器学会学術講演会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2014-04-25
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2013)
  • Author(s): Kurose K, Eikawa S, Mizote Y, Ohue Y, Isobe M, Uenaka A, Oka M, Nakayama E.
  • Organizer: 104nd Annual Meeting of American Association of Cancer Research (AACR)
  • Place of Presentation: Walter E. Washington Convention Center （801 Mt. Vernon Place NW, Washington, DC, USA）
• [Presentation] Immune response to XAGE-1b (GAGED2a) in NSCLC patients and its clinical relevance. (2013)
  • Author(s): Ohue Y, Kurose K, Eikawa S, Mizote Y, Matsumoto H, Isobe M, Uenaka A, Fukuda M, Nakayama E, Oka M.
  • Organizer: 104nd Annual Meeting of American Association of Cancer Research (AACR)
  • Place of Presentation: Walter E. Washington Convention Center （801 Mt. Vernon Place NW, Washington, DC, USA）
• [Presentation] 非小細胞肺癌患者における癌精巣抗原XAGE-1dに対する免疫応答 (2013)
  • Author(s): 黒瀬浩史、松本博文、大植祥弘、阿部公亮、榮川伸吾、溝手 雄、磯辺みどり、上中明子、永安 武、中山睿一、岡 三喜男
  • Organizer: 第53回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区丸の内3丁目5）
• [Presentation] がん精巣抗原XAGE-1bを標的にしたがん免疫療法の開発 (2013)
  • Author(s): 大植祥弘、橘髙 誠、黒瀬浩史、池田征樹、阿部公亮、清水大樹、毛利圭二、加藤茂樹、尾長谷靖、小橋吉博、福田 実、中山睿一、岡 三喜男
  • Organizer: 第53回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区丸の内3丁目5）
• [Presentation] A phase I study of vaccination with 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide. (2013)
  • Author(s): Isobe M, Eikawa S, Kakimi K, Wada H, Mizote Y, Ohue Y, Kurose K, Uenaka A, Oka M, Nakayama E.
  • Organizer: 第17回日本がん免疫学会総会
  • Place of Presentation: ANAクラウンプラザホテル宇部（山口県宇部市相生町8-1）
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2013)
  • Author(s): Kurose K, Eikawa S, Mizote Y, Ohue Y, Isobe M, Uenaka A, Oka M, Nakayama E.
  • Organizer: 第17回日本がん免疫学会総会
  • Place of Presentation: ANAクラウンプラザホテル宇部（山口県宇部市相生町8-1）
• [Presentation] Immune response to XAGE-1b (GAGED2a) in NSCLC patients and its clinical relevance. (2013)
  • Author(s): Ohue Y, Kurose K, Mizote Y, Matsumoto Y, Isobe M, Uenaka A, Fukuda M, Oka M, Nakayama E.
  • Organizer: 第17回日本がん免疫学会総会
  • Place of Presentation: ANAクラウンプラザホテル宇部（山口県宇部市相生町8-1）
• [Presentation] 新規製剤N-propionyl cysteaminylphenol-maleimid-dextran(NPCMD)による免疫応答賦活効果 (2013)
  • Author(s): 溝手 雄、若松一雅、伊藤祥輔、上中明子、黒瀬浩史、大植祥弘、西尾祐美、岡 三喜男、神保孝一、中山睿一
  • Organizer: 第17回日本がん免疫学会総会
  • Place of Presentation: ANAクラウンプラザホテル宇部（山口県宇部市相生町8-1）
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2013)
  • Author(s): Kurose K, Ohue Y, Mizote Y, Isobe M, Uenaka A, Oka M, Nakayama E.
  • Organizer: 第11回日本臨床腫瘍学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県仙台市青葉区青葉山無番地）
• [Presentation] Immune response to XAGE-1b (GAGED2a) in NSCLC patients and its clinical relevance. (2013)
  • Author(s): Ohue Y, Kurose K, Mizote Y, Matsumoto Y, Isobe M, Fukuda M, Uenaka A, Nakayama E, Oka M.
  • Organizer: 第11回日本臨床腫瘍学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県仙台市青葉区青葉山無番地）
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2013)
  • Author(s): Kurose K, Ohue Y, Mizote Y, Isobe M, Uenaka A, Oka M, Nakayama E.
  • Organizer: 21st Annual Cancer Research Institute International Cancer Immunotherapy Symposium
  • Place of Presentation: Grand Hyatt Hotel New York （109 East 42nd Street New York, USA）
• [Presentation] Prolonged overall survival in XAGE-1b(GAGED2a) antibody positive NSCLC patients. (2013)
  • Author(s): Ohue Y, Kurose K, Mizote Y, Matsumoto H, Isobe M, Uenaka A, Fukuda M, Oka M, Nakayama E.
  • Organizer: 21st Annual Cancer Research Institute International Cancer Immunotherapy Symposium
  • Place of Presentation: Grand Hyatt Hotel New York （109 East 42nd Street New York, USA）
• [Presentation] NY-ESO-1重複長ペプチドを用いたがんワクチン第I相臨床試験 (2013)
  • Author(s): 西塔拓郎、和田 尚、磯辺みどり、垣見和宏、榮川伸吾、大植祥弘、西川博嘉、岡 三喜男、森 正樹、土岐祐一郎、中山睿一
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市西区みなとみらい1-1-1）
• [Presentation] NY-ESO-1(NY-ESO-1 91-110)ペプチドワクチンによる抗体CD4・CD8 T細胞免疫応答の誘導 (2013)
  • Author(s): 榮川伸吾、垣見和宏、磯辺みどり、和田 尚、上中明子、葛島清隆、西川博嘉、鵜殿平一郎、岡 三喜男、中山睿一
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市西区みなとみらい1-1-1）
• [Presentation] 新規製剤NPCMDによるTLR4、NLRP3の活性化とT細胞免疫応答の賦活化 (2013)
  • Author(s): 溝手 雄、若松一雅、伊藤祥輔、上中明子、黒瀬浩史、大植祥弘、西尾祐美、岡 三喜男、神保孝一、中山睿一
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市西区みなとみらい1-1-1）
• [Presentation] ホジキンリンパ腫におけるNY-ESO-1 に対する液性および細胞性免疫応答 (2013)
  • Author(s): 鈴木 進、石田高司、森 芙美子、伊藤 旭、楠本 茂、小松弘和、吉川和宏、中山睿一、飯田真介、新実彰男、上田龍三
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市西区みなとみらい1-1-1）
• [Presentation] Depletion of Tregs from PBMCs by treatment with defucosylated anti-CCR4 mAb. (2013)
  • Author(s): 黒瀬浩史、大植祥弘、堅田洋佑、阿部公亮、磯辺みどり、松本博文、上中明子、中山睿一、岡 三喜男
  • Organizer: 第54回日本肺癌学会総会
  • Place of Presentation: ホテルニューオータニ東京（東京都千代田区紀尾井町4-1）
• [Presentation] Immune response to XAGE-1b (GAGED2a) in NSCLC patients and its clinical relevance. (2013)
  • Author(s): 大植祥弘、黒瀬浩史、松本博文、阿部公亮、堅田洋佑、磯辺みどり、上中明子、福田 実、中山睿一、岡 三喜男
  • Organizer: 第54回日本肺癌学会総会
  • Place of Presentation: ホテルニューオータニ東京（東京都千代田区紀尾井町4-1）
• [Remarks] 免疫研究室
  • URL: http://www.med-gakkai.org/kmsrd/igakukyoiku.html