| Project/Area Number |
25430196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservation of biological resources
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Research Collaborator |
KITANISHI Shigeru
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 遺伝子型性 / 表現型性 / サクラマス / 偽成長ホルモン遺伝子 / 成長抑制 / 性判別 / リアルタイムＰＣＲ / 耳石 / 性決定 / 繁殖形質 / 成長様式 / Y染色体特異的マーカー |
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| Outline of Final Research Achievements |
The aim of this research was to evaluate the distribution patterns and appearance mechanism of individuals with incongruence between genotypic and phenotypic sex. As the results of study of distribution, the incongruent individuals were found only in the populations of Hokkaido, not in those of Tyugoku and Tohoku districts. This result indicates that incongruent individuals are only distributed with Hokkaido area near the Sea of Japan. In secondary study, fertilization test using incongruent individuals showed that sex ratio were male-biased, suggesting Y-to-X chromosomal translocation involving GHY genetic markers. Moreover, we are trying to evaluate the number of GHY genetic markers using the real time PCR, but techniques of estimation have not been established yet at the present.
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