Folding mechanism of non-two-state proteins based on correlation analyses of kinetic folding data
| Project/Area Number |
25440075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo (2015-2016)
The Graduate University for Advanced Studies (2013-2014) |
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Principal Investigator |
Kuwajima Kunihiro 東京大学, 大学院理学系研究科(理学部), 名誉教授 (70091444)
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| Research Collaborator |
LEE Jooyoung 韓国高等科学院, 教授
MANAVALAN Balachandran 韓国高等科学院, 研究員
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | フォールディング / 速度論 / データベース |
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| Outline of Final Research Achievements |
We carried out literature searching on the folding kinetics of globular proteins, and constructed a protein-folding database. The total entry number of the database is 141, consisting of 59 non-two-state proteins and 82 two-state proteins. We took the non-two-state proteins and the two-state proteins separately from the database, and investigated correlations between the logarithmic rate constant (ln kf) of folding and the structural properties of proteins. We show that using the logarithmic number of nonlocal contact clusters, a structural property proposed by us, we can clearly describe differences in the molecular mechanisms of folding between the non-two-state and the two-state proteins.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Dissection of the ATP-dependent conformational change cycle of a group II chaperonin.2014
Author(s)
Nakagawa A, Moriya K, Arita M, Yamamoto Y, Kitamura K, Ishiguro N, Kanzaki T, Oka T, Makabe K, Kuwajima K, Yohda M.
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Journal Title
J Mol Biol.
Volume: 426
Issue: 2
Pages: 447-459
DOI
Related Report
Peer Reviewed
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[Journal Article] ATP dependent rotational motion of group II chaperonin observed by X-ray single molecule tracking.2013
Author(s)
Sekiguchi H, Nakagawa A, Moriya K, Makabe K, Ichiyanagi K, Nozawa S, Sato T, Adachi S, Kuwajima K, Yohda M, Sasaki YC.
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Journal Title
PLoS One
Volume: 8
Issue: 5
Pages: e64176-e64176
DOI
Related Report
Peer Reviewed
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[Presentation] The problem of protein folding2016
Author(s)
Kuwajima, K., Manavalan, B. & Lee, J.
Organizer
The 16th KIAS Conference on Protein Structure
Place of Presentation
Seoul (Korea)
Year and Date
2016-09-22
Related Report
Int'l Joint Research / Invited
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[Presentation] The problem of protein folding2016
Author(s)
Kuwajima, K., Manavalan, B. & Lee, J.
Organizer
The 2nd Polish-Korean Conference on Protein Folding: Theoretical and Experimental Approaches
Place of Presentation
Gdansk (Poland)
Year and Date
2016-05-28
Related Report
Int'l Joint Research / Invited
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[Presentation] The H/D-exchange kinetics of a fully unfolded protein studied by the DMSO-quenched and 2D NMR techniques2016
Author(s)
Kuwajima, K., Chandak, M.S., Yagi-Utsumi, M., Yamaguchi, T., Nakamura, T. & Kato, K.
Organizer
The 8th Japan-Korea Seminar on Biomolecular Science: Experiments and Simulations
Place of Presentation
自然科学研究機構・岡崎コンファレンスセンター（愛知県岡崎市）
Year and Date
2016-02-15
Related Report
Invited
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[Presentation] The problem of protein folding2015
Author(s)
Korea Institute for Advanced Study (KIAS), Seoul, Korea
Organizer
The 14th FAOBMB Congress
Place of Presentation
CSIR-Centre for Cellular & Molecular Biology (CCMB), Hyderabad, India
Year and Date
2015-11-27
Related Report
Int'l Joint Research / Invited
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