| Project/Area Number |
25440090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Takasaki University of Health and Welfare |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UMATA Toshiyuki 産業医科大学, 産業医学支援機構, 准教授 (30213482)
OKAMOTO Kengo 高崎健康福祉大学, 薬学部, 講師 (60437754)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リボソームRNA遺伝子 / ヘテロクロマチン / クロマチン / ヒストン / メチル化修飾 / H4K20me3 / ボソームRNA遺伝子 / KDM2A / SF-KDM2A |
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| Outline of Final Research Achievements |
There are two types of chromatin structure, euchromatin and heterochromatin in eukaryotes. These structures are exchangeable, but the molecular mechanisms are not clear. In this study, we attempted to identify a molecule specific for heterochromatin and investigate the mechanism of controlling the molecule in ribosomal RNA gene (rDNA) promoter. First our data suggested that HP1, which bound to H3K9me3, was not a heterochromatin mark in rDNA promoter. Second, SF-KDM2A, which was expressed from the gene encoding histone demethylase KDM2A and did not possess the demethylase activity, reduced H4K20me3 in rDNA promoter. We found that SF-KDM2A controlled accumulation of Suv4-20h2, which resulted in elevation or reduction of Suv4-20h2, and the activities may be affected by cell culturing conditions. Our data suggest that SF-KDM2A can control the chromatin structure of rDNA in response to environmental conditions.
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