Study on roles of G protein and PLC singnalings in plant endoplasmic reticulum stress tolerance

• Project/Area Number: 25440123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Muroran Institute of Technology
• Principal Investigator: KANEHARA Kazue 室蘭工業大学, 工学(系)研究科(研究院), その他(客員准教授) (30587746)
• Co-Investigator(Kenkyū-buntansha): IWASA Tatsuo 室蘭工業大学, 工学研究科, 教授 (00133926)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ストレス応答 / 高等植物 / 小胞体 / Gタンパク質 / PLC / Arabidopsis

Outline of Final Research Achievements

Plants have evolved molecular mechanisms to adapt themselves to environmental changes using various strategies. Recent investigations revealed cellular mechanisms, particularly at the endoplasmic reticulum, in response to environmental stresses including high temperature and high salinity. In our research project, we found that heterotrimetic G proteins and lipid synthesis enzyme played crucial roles for endoplasmic reticulum stress tolerance in a model higher plant Arabidopsis thaliana. These findings were published as three original research articles in international scientific journals.

Research Products

• [Int'l Joint Research] National University of Singapore/Singapore(Singapore)
• [Journal Article] Arabidopsis AtPLC2 is a primary phosphoinositide-specific phospholipase C in phosphoinositide metabolism and the endoplasmic reticulum stress response (2015)
  • Author(s): Kazue Kanehara, Chao-Yuan Yu, Yueh Cho, Wei-Fun Cheong, Federico Torta, Guanghou Shui, Markus R Wenk and Yuki Nakamura
  • Journal Title: PLOS Genetics Volume: 11 Issue: 9 Pages: 1-19
  • DOI: 10.1371/journal.pgen.1005511
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Heterotrimeric G protein subunits differentially respond to endoplasmic reticulum stress in Arabidopsis (2015)
  • Author(s): Y. Cho, C. –Y. Yu, T. Iwasa and K. Kanehara
  • Journal Title: Plant Signaling & Behavior Volume: 10 Issue: 10 Pages: 1-10
  • DOI: 10.1080/15592324.2015.1061162
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] DOK1 encodes a functional dolichol kinase involved in reproduction (2015)
  • Author(s): K. Kanehara, Y. Cho, Y-C. Lin, C-E. Chen, C-Y. Yu and Y. Nakamura
  • Journal Title: Plant Journal Volume: 81 Issue: 2 Pages: 292-203
  • DOI: 10.1111/tpj.12727
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Phosphoinositide-specific phospholipase C2, AtPLC2, in phosphoinositide metabolism and the endoplasmic reticulum stress response (2016)
  • Author(s): Kazue Kanehara
  • Organizer: 第５７回日本植物生理学会年会
  • Place of Presentation: 盛岡大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Arabidopsis AtPLC2 is a primary phosphoinositide-specific phospholipase C in phosphoinositide metabolism and the endoplasmic reticulum stress response (2015)
  • Author(s): Kazue Kanehara
  • Organizer: International Singapore Lipid Symposium (ISLS6) & Asian Symposium on Plant Lipids (ASPL)
  • Place of Presentation: シンガポール大学 （シンガポール、シンガポール）
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] Arabidopsis DOK1 encodes a functional dolichol kinase involved in reproductive processes (2015)
  • Author(s): Yueh Cho, Chao-Yuan Yu and Kazue Kanehara
  • Organizer: The 56th Annual Meeting of the Japanese Society of Plant Physiologists
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Arabidopsis DOK1 encodes a functional dolichol kinase in protein glycosylation (2014)
  • Author(s): Kazue Kanehara, Chia-En Chen, Ying-Chen Lin, Tatsuo Iwasa
  • Organizer: Annual scientific meeting of the American Society of Plant Biolosisits 2014
  • Place of Presentation: Oregon Convention Center, Portland, USA
  • Year and Date: 2014-07-12 – 2014-07-16
• [Presentation] A phospholipase C involved in phosphoinositides metabolism and endoplasmic reticulum homeostasis (2013)
  • Author(s): Kazue Kanehara, Chia-En Chen, Yu-Chi Liu, Wei-Fun Cheong, Guanghou Shui, Tatsuo Iwasa, Markus Wenk, Yuki Nakamura
  • Organizer: 6th European Symposium on Plant Lipids
  • Place of Presentation: IBIS Bordeaux Centre Meriadeck Hotel, Bordeaux, FRANCE
• [Presentation] Phosphoinositides metabolism is involved in growth and circumvention of ER stress in Arabidopsis (2013)
  • Author(s): Kazue Kanehara, Chia-En Chen, Yu-Chi Liu, Wei-Fun Cheong, Guanghou Shui, Tatsuo Iwasa, Markus Wenk, Yuki Nakamura
  • Organizer: 31st Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology
  • Place of Presentation: Hokkaido University, Sapporo, JAPAN