Analysis of calcium signaling network in plants and alteration of the substrate specificity of protein kinase using plant CDPK

Research Project

Project/Area Number	25440126
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	The University of Tokyo
Principal Investigator	Ishida Sarahmi 東京大学, 理学(系)研究科(研究院), 助教 (20282725)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	情報伝達系 / カルシウム / タンパク質キナーゼ / 基質特異性 / CDPK / 情報伝達 / カルシウム情報伝達 / リン酸化
Outline of Final Research Achievements	We have demonstrated that Calcium-dependent protein kinases (CDPK) in plants could be divided to two domains, that is, substrate recognition domain and catalytic domain and estimated CDPKs were suitable as the model system in analysis for altering the substrate specificity of protein kinases. The success in altering substrate specificity of protein kinases is expected to promote a novel signal transduction pathway constructed theoretically. In this research, the mechanism for specific substrate recognition process in plant CDPK was analysed in detail aiming establishment of an examination system for altering the substrate specificity of protein kinases in signal transduction in cells.