Analysis of the factors of CHUP1 signaling complex involved in chloroplast photorelocation movement

• Project/Area Number: 25440140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: KONG SAMGEUN 九州大学, 学内共同利用施設等, 助教 (70514157)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 葉緑体運動 / 青色光 / 光受容体 / フォトトロピン / アクチン繊維 / 信号伝達

Outline of Final Research Achievements

Chloroplast photorelocation movement is an essential physiological response for plants to optimize their photosynthetic ability and to avoid photodamage under fluctuating environmental light conditions. Chloroplasts can move to any directions according to the intensity and direction of incident light. Chloroplast movement is dynamically mediated by rapid reorganizations of chloroplast actin filament (cp-actin) on a chloroplast outer envelope. In this study, I analyzed the regulation mechanism of dynamic reorganizations of cp-actin filaments that taking place on the chloroplast outer envelope through the blue-light-dependent interaction between the molecules of the CHUP1 signaling complex. Chloroplast anchoring to the plasma membrane and bundling of cp-actin filaments were reversibly regulated by the blue-light-dependent phosphorylation of the CHUP1 signaling complex that were regulated by phot2.

Research Products

• [Journal Article] Distinct biochemical properties of Arabidopsis thaliana actin isoforms. (2016)
  • Author(s): Kijima, S. T., Hirose, K., Kong, S. G. Wada, M. and Uyeda, T.Q.P.
  • Journal Title: Plant and Cell Physiology Volume: 57 Issue: 1 Pages: 46-56
  • DOI: 10.1093/pcp/pcv176
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Diverse photoreceptors and light responses in plants (2016)
  • Author(s): Kong SG, Okajima K
  • Journal Title: J. Plant Res. Volume: 129 Issue: 2 Pages: 111-114
  • DOI: 10.1007/s10265-016-0792-5
  • Open Access / Int'l Joint Research
• [Journal Article] Molecular basis of chloroplast photorelocation movement (2016)
  • Author(s): Kong S.-G., Wada M.
  • Journal Title: Journal of Plant Research Volume: 129 Issue: 2 Pages: 159-166
  • DOI: 10.1007/s10265-016-0788-1
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] PLASTID MOVEMENT IMPAIRED1 and PLASTID MOVEMENT IMPAIRED-LIKE 1 mediate photorelocation movement of both chloroplasts and nuclei. (2015)
  • Author(s): Suetsugu, N., Higa, T., Kong, S.-G. and Wada, M.
  • Journal Title: Plant Physiology Volume: 169 Issue: 2 Pages: 1155-1167
  • DOI: 10.1104/pp.15.00214
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Photo-Induced Oligomerization of Arabidopsis Thaliana Phototropin 2 LOV1 (2014)
  • Author(s): Y. Nakasone, Y. Kawaguchi, S-G. Kong, M. Wada, M. Terazima
  • Journal Title: J.Phys.Chem.B Volume: 118 Issue: 49 Pages: 14314-14325
  • DOI: 10.1021/jp509448b
  • Peer Reviewed / Open Access
• [Journal Article] Recent advances in understanding the molecular mechanism of chloroplast photorelocation movement. (2014)
  • Author(s): Kong, S.-G., and M. Wada
  • Journal Title: Biochi. Biophsi. Acta Volume: 1837 Issue: 4 Pages: 522-530
  • DOI: 10.1016/j.bbabio.2013.12.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Actin-dependent plastid movement is required for motive force generation in directional nuclear movement in plants. (2014)
  • Author(s): Higa, T., N. Suetsugu, S.-G. Kong, and M. Wada
  • Journal Title: Proc. Natl Acad. Sci. USA Volume: 111 Issue: 11 Pages: 4327-4331
  • DOI: 10.1073/pnas.1317902111
  • Peer Reviewed / Open Access
• [Journal Article] Interaction between avoidance of photon absorption, excess energy dissipation and zeaxanthin synthesis against photoxidative stress in Arabidopsis． (2013)
  • Author(s): Cazzaniga, S., L. Dall’Osto, S.-G. Kong, M. Wada, and R. Bassi
  • Journal Title: Plant J. Volume: 76 Issue: 4 Pages: 568-579
  • DOI: 10.1111/tpj.12314
  • Peer Reviewed
• [Journal Article] Observation of chloroplast-actin filaments in leaves of Arabidopsis (2013)
  • Author(s): Sam-Geun Kong and Masamitsu Wada
  • Journal Title: bio-protocol Volume: 3 Pages: 1-1
  • Peer Reviewed
• [Presentation] 葉緑体は光に応じてどのように細胞内を移動するのか？ (2016)
  • Author(s): 孔三根
  • Organizer: 第１８回植物オルガネラワークショップ
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-17
  • Invited
• [Presentation] Molecular dynamics in chloroplast photorelocation movement. (2015)
  • Author(s): Kong S.-G.
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] 葉緑体光定位運動の分子機構 (2015)
  • Author(s): 孔三根
  • Organizer: Plant Cytoskeleton 2015
  • Place of Presentation: 三島
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] The biochemical role of CHLOROPLAST UNUSUAL POSITIONING 1 in chloroplast photorelocation movement (2015)
  • Author(s): Sam-Geun Kong, Saku Kijima, Atsushi Shimada, Noriyuki Suetsugu, Keiko Hirose, Fumio Takahashi, Daisuke Kohda, Taro Q. P. Uyeda, and Masamitsu Wada
  • Organizer: 第５６回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Chloroplast Unusual Positioning 1 Is A Plant-Specific Class of Actin Nucleator for Chloroplast Photorelocation Movement (2015)
  • Author(s): Sam-Geun Kong, Saku Kijima, Atsushi Shimada, Noriyuki Suetsugu, Keiko Hirose, Fumio Takahashi, Daisuke Kohda, Taro Q. P. Uyeda, and Masamitsu Wada
  • Organizer: "The 2nd International Symposium on Plant Environmental Sensing"
  • Place of Presentation: 産業技術統合研究所（東京都）
  • Year and Date: 2015-03-13 – 2015-03-15
• [Presentation] Regulation mechanism of CHUP1 signaling complex for chloroplast photorelocation movement (2014)
  • Author(s): Sam-Geun Kong
  • Organizer: 第７８回日本植物学会年会
  • Place of Presentation: 明治大学（東京都）
  • Year and Date: 2014-09-12 – 2014-09-14
  • Invited