| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In order to clarify the molecular mechanism by which plants cope with DNA-damaging stresses that endanger the genome stability, we have analyzed the DNA polymerases that specifically replicate damaged DNA. We found that translesion synthesis (TLS)-type DNA polymerases interact each other and also with DNA replication clamp. We also found that the frequency of homologous recombination was significantly increased by ultraviolet light irradiation when either AtRev1 or AtPolζ was disrupted. Furthermore, both mutation and recombination frequencies were affected when plants were grown in the presence of a heat shock protein (HSP) inhibitor, suggesting HSPs are involved in two pathways.
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