Mechanisms underlying the formation of syncitial endosperm using endspermless mutants in rice
Project/Area Number |
25450005
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Science in genetics and breeding
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Research Institution | Nagoya University |
Principal Investigator |
HATTORI TSUKAHO 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
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Co-Investigator(Renkei-kenkyūsha) |
NAGATO Yasuo 東京大学, 名誉教授 (10143413)
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Research Collaborator |
KATO Hirokazu
KUROTANI Ken-ichi
MIYATA Yasuhiro
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 胚乳 / 穀類 / 核 / イネ / 多核細胞 / 細胞分裂 / アリューロン細胞 / デンプン性胚乳 / シンシチウム / 種子 / 染色体 |
Outline of Final Research Achievements |
In many angiosperms, the primary endosperm nucleus produced by double fertilization undergoes division without cytokinesis resulting in the formation of syncytial nuclear endosperm. In rice nucler endosperm with several thousands of free nuclei is observed 3 days after pollination. To obtain deeper insight into the regulatory mechanism, we investigated a rice endospermless mutant, enl2, which lacks endosperm tissues in its mature seeds. We found that the mutant had defects in chromosome segregation and separation of free nuclei. In addition, we identified a candidate causal gene of enl2 by NGS. The gene encoded a protein, of which orthologs in some lower eukaryote including Chlamydomonas have been reported to function in nuclear envelope fusion and meiosis.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development.2015
Author(s)
Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagawa, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori
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Journal Title
The Plant Journal
Volume: 81
Issue: 1
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cell-by-cell developmental transition from embryo to postgermination phase revealed by heterochronic gene expression and ER-body formation in Arabidopsis leafy cotyledon mutants.2014
Author(s)
Yamamoto, A., Yoshii, M., Murase, S., Fujita, M., Kurata, N., Hobo, T., Kagaya, Y., Takeda, S. and Hattori, T.
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Journal Title
Plant & Cell Physiology
Volume: 55
Issue: 12
Pages: 2112-2125
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Characterization of rice seed mutants --- toward identification of mutated loci using NGS.2014
Author(s)
1.Kurotani, K., Katoh, H., Suzuki, T., Kobayashi, Y., Kobayashi, Sato, Y., Nagato, Y., Kitano, H., Yoon, I.S., Takeda, T. and Hattori, T.
Organizer
XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
Place of Presentation
Tokyo
Year and Date
2014-10-27 – 2014-10-29
Related Report
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[Presentation] Rice ENDOSPERMLESS1, an SNF2 family chromatin remodeling helicase, plays an essential role in nuclear proliferation during syncytial endosperm development in rice.2014
Author(s)
2.Katoh, H., Hara, T., Ogawa, D., Kagaya, Y., Sato, Y., Kitano, H., Nagato, Y., Ishikawa, R., Ono, A., Kinoshita, T., Kruotani, K., Miyata, Y., Nishio, M., Kojima, S., Takeda, S. and Hattori, T.
Organizer
XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
Place of Presentation
Tokyo
Year and Date
2014-10-27 – 2014-10-29
Related Report
Invited
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