Mechanisms underlying the formation of syncitial endosperm using endspermless mutants in rice

• Project/Area Number: 25450005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Nagoya University
• Principal Investigator: HATTORI TSUKAHO 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
• Co-Investigator(Renkei-kenkyūsha): NAGATO Yasuo 東京大学, 名誉教授 (10143413)
• Research Collaborator: KATO Hirokazu ; KUROTANI Ken-ichi ; MIYATA Yasuhiro
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 胚乳 / 穀類 / 核 / イネ / 多核細胞 / 細胞分裂 / アリューロン細胞 / デンプン性胚乳 / シンシチウム / 種子 / 染色体

Outline of Final Research Achievements

In many angiosperms, the primary endosperm nucleus produced by double fertilization undergoes division without cytokinesis resulting in the formation of syncytial nuclear endosperm. In rice nucler endosperm with several thousands of free nuclei is observed 3 days after pollination. To obtain deeper insight into the regulatory mechanism, we investigated a rice endospermless mutant, enl2, which lacks endosperm tissues in its mature seeds. We found that the mutant had defects in chromosome segregation and separation of free nuclei. In addition, we identified a candidate causal gene of enl2 by NGS. The gene encoded a protein, of which orthologs in some lower eukaryote including Chlamydomonas have been reported to function in nuclear envelope fusion and meiosis.

Research Products

• [Journal Article] The Arabidopsis transcription factor NAI1 is required for enhancing the active histone mark but not for removing the repressive mark on PYK10, a seedling–specific gene upon embryonic-to-postgerminative developmental phase transition. (2015)
  • Author(s): Yoshii, M., Yamamoto, A., Kagaya, Y., Takeda S. and Hattori, T.
  • Journal Title: Plant Signal. Behav. Volume: 10 Issue: 12 Pages: e1105418-e1105418
  • DOI: 10.1080/15592324.2015.1105418
  • Peer Reviewed
• [Journal Article] Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development. (2015)
  • Author(s): Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagawa, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori
  • Journal Title: The Plant Journal Volume: 81 Issue: 1 Pages: 1-12
  • DOI: 10.1111/tpj.12705
  • Peer Reviewed / Open Access
• [Journal Article] Cell-by-cell developmental transition from embryo to postgermination phase revealed by heterochronic gene expression and ER-body formation in Arabidopsis leafy cotyledon mutants. (2014)
  • Author(s): Yamamoto, A., Yoshii, M., Murase, S., Fujita, M., Kurata, N., Hobo, T., Kagaya, Y., Takeda, S. and Hattori, T.
  • Journal Title: Plant & Cell Physiology Volume: 55 Issue: 12 Pages: 2112-2125
  • DOI: 10.1093/pcp/pcu139
  • Peer Reviewed / Open Access
• [Journal Article] BRU1 maintains configuration of the euchromatic subchromosomal domain in the nucleus of Arabidopsis. (2014)
  • Author(s): Ohno, Y, Nishimura, T., Hattori, T., and Takeda, S.
  • Journal Title: Plant Mol. Biol. Rep. Volume: 32 Issue: 1 Pages: 19-27
  • DOI: 10.1007/s11105-013-0596-x
  • Peer Reviewed
• [Journal Article] 種子形成シナリオの分子遺伝学 (2013)
  • Author(s): 伊藤純一、佐藤豊、桧原健一郎、服部束穂、岩崎行弦
  • Journal Title: 育種学研究 Volume: 15 Pages: 128-133
  • NAID: 10031191382
• [Presentation] アリューロンが多層化するイネ胚乳変異体abnormal aleurone layer 1 (aal1) の解析. (2015)
  • Author(s): 加藤大和、深井麻央、黒谷賢一、佐藤豊、北野英己、長戸康郎、小林裕子、小林一成、武田真、服部束穂
  • Organizer: 日本育種学会第128回講演会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] イネ胚乳発生異常変異体endosperm defective 1 (end1)の原因遺伝子はシキミ酸経路初発酵素をコードする. (2015)
  • Author(s): 黒谷賢一、加藤大和、高藤良典、小林裕子、小林一成、佐藤豊、桧原健一郎、長戸康郎、北野英己、In-Sun Yoon、武田真、服部束穂
  • Organizer: 日本育種学会第128回講演会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] Characterization of rice seed mutants --- toward identification of mutated loci using NGS. (2014)
  • Author(s): 1.Kurotani, K., Katoh, H., Suzuki, T., Kobayashi, Y., Kobayashi, Sato, Y., Nagato, Y., Kitano, H., Yoon, I.S., Takeda, T. and Hattori, T.
  • Organizer: XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
  • Place of Presentation: Tokyo
  • Year and Date: 2014-10-27 – 2014-10-29
• [Presentation] Rice ENDOSPERMLESS1, an SNF2 family chromatin remodeling helicase, plays an essential role in nuclear proliferation during syncytial endosperm development in rice. (2014)
  • Author(s): 2.Katoh, H., Hara, T., Ogawa, D., Kagaya, Y., Sato, Y., Kitano, H., Nagato, Y., Ishikawa, R., Ono, A., Kinoshita, T., Kruotani, K., Miyata, Y., Nishio, M., Kojima, S., Takeda, S. and Hattori, T.
  • Organizer: XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
  • Place of Presentation: Tokyo
  • Year and Date: 2014-10-27 – 2014-10-29
  • Invited
• [Presentation] 種子休眠と発芽 --- 胚成長の停止と再開という視点から (2014)
  • Author(s): 加賀谷安章、脇坂恵、加賀谷道子、岩田昇悟、加藤大和、山本章子、武田真、服部束穂
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
  • Invited
• [Presentation] カルスからの再分化過程に異常を示す新規エピ変異体の探索 (2014)
  • Author(s): 西村泰介、山本章子、武田真、服部束穂
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] イネの胚乳形成について (2013)
  • Author(s): 服部束穂
  • Organizer: 第34回種子生理生化学研究会年会
  • Place of Presentation: 箱根
  • Invited