Analysis of the insecticide detoxification of insecticide resistance by the cytochrome P450 new expression method

• Project/Area Number: 25450076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Komagata Osamu 国立感染症研究所, その他部局等, 研究員 (20435712)
• Co-Investigator(Kenkyū-buntansha): Tomita Takashi 国立感染症研究所, 昆虫医科学部, 主任研究官 (20180169)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 殺虫剤抵抗性

Outline of Final Research Achievements

P450s are important detoxication enzymes in insecticide resistance. The P450s are proteins of the superfamily. Each insect has many P450 isozymes. However, the functions of each P450 were still not clear. The P450s from a high pyrethroid resistant strain of the southern house mosquito (Culex pipiens quinquefasciatus) was attempted to be expressed by the baculovirus display system for in vitro assay. The P450 coding cDNA was synthesized by the reverse transcription from mosquito’s total RNA. The virus for expression was created although it was hard to make the constructions from P450 and P450 reductase cDNA. The vectors were transfected into insect cells. Expressed P450 was harvested from the envelope of budded virus after cell culturing with the virus; however, the yield was not adequate. In this case, optimization of expression is deemed as a future task.