Study on function and regulation of zinc for embryo development and seed maturation of rice plant

• Project/Area Number: 25450080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Utsunomiya University
• Principal Investigator: Takahashi Michiko 宇都宮大学, 農学部, 准教授 (90345182)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: イネ / 亜鉛 / 卵細胞 / 胚発生

Outline of Final Research Achievements

One transporter gene which expression increased 13 times after fertilization was determined by microarray analysis using rice eggs before and after fertilization. The tos mutant of this transporter presented the phenotype of parthenocarpy. Measurement of IAA concentration in ovaries revealed that IAA concentration in Tos mutant was lower than wild type rice plant. Analysis of metal concentrations in plant body revealed that zinc concentration in ovaries of Tos mutant was significantly higher than that of wild type rice plant.

Research Products

• [Journal Article] Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice (2015)
  • Author(s): K. Bashir, Y. Ishimaru, R. Nakanishi Itai, T. Senoura, M. Takahashi, G. An, T. Oikawa, M. Ueda, A. Sato, N. Uozumi, H. Nakanishi, and N. K Nishizawa
  • Journal Title: Plant Mol. Biol. Volume: 88 Issue: 1-2 Pages: 165-176
  • DOI: 10.1007/s11103-015-0315-0
  • Peer Reviewed
• [Journal Article] Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean. (2014)
  • Author(s): Tomoko Nozoye, Suyoen Kim, Yusuke Kakei, Michiko Takahashi, Hiromi Nakanishi & Naoko K. Nishizawa
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 78 Pages: 1677-1684
  • Peer Reviewed
• [Journal Article] Rice nicotianamine synthase localizes to particular vesicles for proper function (2014)
  • Author(s): Nozoye T, Tsunoda K, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK
  • Journal Title: Plant Signaling and Behavior Volume: 9 Issue: 5 Pages: e28660-e28660
  • DOI: 10.4161/psb.28660
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice. (2014)
  • Author(s): Nozoye T, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK.
  • Journal Title: The Plant Journal Volume: 77 Pages: 246-260
  • Peer Reviewed
• [Journal Article] Rice (Oryza sativa L.) roots have iodate reduction activity in response to iodine (2013)
  • Author(s): Kato, S., Wachi, T., Yoshihira, K., Nakagawa, T., Ishikawa, A., Takagi, D., Tezuka, A., Yoshida, H., Yoshida, S., Sekimoto, H. and Takahashi, M
  • Journal Title: Frontiers in Plant Sci. Volume: 4
  • DOI: 10.3389/fpls.2013.00227
  • Peer Reviewed
• [Presentation] Analysis of functions of AtbZIP19 in rice plant (2014)
  • Author(s): Aiko Watanabe, Hirokuni Ikezawa, Tetsuya Shimoyama, Vanessa Stein, Mark GM Aarts, Michiko Takahashi
  • Organizer: the 17th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP)
  • Place of Presentation: ドイツ（Gatersleben/Quedlinburg）
  • Year and Date: 2014-07-07
• [Presentation] Rice plants have an ability to reduce IO3- to I- in the rhzpsphere, and transport iodine in the form of both I- and IO3- in xylem.
  • Author(s): Shota Kato, Takanori Wachi, Masataka Kato, Kei Yoshihira, Akifumi Ishikawa, Daichi Takagi, Aya Tedsuka, Takuya Nakagawa, Hideharu Yoshida, Futoshi Miyazawa, Satoshi Yoshida, Michiko Takahashi, Hitoshi Sekimoto
  • Organizer: XVII International Plant Nutrition Colloquium
  • Place of Presentation: Istanbul Convention and Exhibition Center
• [Presentation] シロイヌナズナのトランスポーターAtCLCaのヨウ素輸送とヨウ素応答
  • Author(s): 栗林美早、加藤翔太、下山哲也、中川拓也、吉田聡、関本均、Sebastien Thomine, Sophie Filleur, 高橋美智子
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] ヨウ素過剰耐性イネ変異体のヨウ素吸収・移行の解析
  • Author(s): 中川拓也、吉平恵依、加藤翔太、伊藤純一、長戸康郎、吉田聡、関本均、高橋美智子
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 作物間におけるヨウ素過剰耐性と植物体ヨウ素濃度の比較解析
  • Author(s): 中川拓也、渡辺健太、猪狩浩明、加藤翔太、石川明史，高木大地、手塚彩、吉田聡、関本均、高橋美智子
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] イネ導管液中ヨウ素の化学形態分析
  • Author(s): 加藤 翔太、加藤 雅隆、宮澤 太志、中川 拓也、吉田 聡、高橋 美智子、関本 均
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 名古屋大学