| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we tried to improve thermotolerance of S.cerevisiae by modified genome shuffling. In the first round of genome shuffling, S. cerevisiae TJ14 and C3867 strains showing thermotolerance up to 41°C with high ethanol productivity were used as recipient strains. Non-conventional yeast Hansenula polymorpha showing robust thermotolerance up to 49°C was chosen as donor strains. After transformation of recipient strains with genomic DNA from donor strain, 3 transformants were isolated at 42°C and they showed pretty better growth and 7 times higher ethanol production than their recipient strains at 42°C. In the second round of genome shuffling, 2 transformants were isolated at 42.5°C and they showed significantly better growth and 2 times higher ethanol production than their recipient strains at 42.5°C. These strains will contribute to providing better platforms for further development of thermotolerant yeast for bioethanol production.
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