Cell surface expression of proteins using Pichia yeast: Development and application of high-throuput construction of expression system
Project/Area Number |
25450126
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | Kyoto University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | タンパク質工学 / Pichia pastoris / 発現系構築 / X線結晶構造解析 |
Outline of Final Research Achievements |
Using High-throuput method constructing Pichia yeast protein expression system, cell surface expression of green fluorescence protein and the target protein using Pichia yeast has been achieved. Pichia yeast producing alpha-amylase on the cell surface had starch diesting activity. Using Pichia yeast, cellulose digesting enzymes of molluscs were produced. For the enzyme of a snail, molecular structure and catalytic mechanism were analyzed.
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Report
(5 results)
Research Products
(2 results)