Study on structure, function and gene regulation of Protobothrops flavoviridis snake venom phospholipase A2 isozymes
| Project/Area Number |
25450139
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 仁美 崇城大学, 薬学部, 助教 (60510691)
大栗 誉敏 崇城大学, 薬学部, 准教授 (70346807)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBATA Hironori 九州大学, 生体防御医学研究所, 准教授 (80315093)
UNOKI Motoko 九州大学, 生体防御医学研究所, 助教 (30525374)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 蛇毒 / ホスホリパーゼA2 / 遺伝子発現 / ホスホリパーゼ / 発現制御機構 / 転写調節 / venom / phopholipase A2 / transcription |
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| Outline of Final Research Achievements |
We have studied the structure, function and gene expression of venomous phospholipase A2 (PLA2) isozymes in habu snake (Protobothrops flavoviridis), especially focusing on specifically expressed PLA2 isozyme, so-called pgPLA1b/2b, in juvenile snake. For this purpose, pgPLA1b/2b protein was expressed in yeast and purified on column chromatographies. The gene regulation study with RT-PCR for venom glands of various ages demonstrated that expression of pgPLA1b/2b decreased as the age of the snake increased from 0 to 1 year old and it was no longer detected in 2-year-old-snakes. On the other hand, four other PLA2 isozyme genes were steadily expressed regardless of age. This work presents new perspectives as to why pgPLA1b/2b exists only in juvenile snake and how it can be regulated in gene expression with age.
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Report
(5 results)
Research Products
(31 results)
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[Presentation] Juvenile snake specific expression of a novel phospholipase A2 isozyme gene in Protobothrops flavoviridis venom gland.2016
Author(s)
Nakamura, H., Murakami T., Hattori, S., Ohkuri, T., Ohno, M., Oda-Ueda T.
Organizer
XII Congress of the Pan-American Section of the International Society on Toxinology
Place of Presentation
Miami Beach, Florida, USA
Year and Date
2016-09-18
Related Report
Int'l Joint Research
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[Presentation] Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes.2015
Author(s)
Nakamura H., Murakami T., Hattori S., Sakaki Y., Ohkuri, T., Chijiwa T., Ohno M., Oda-Ueda N.
Organizer
The 18th world congress of the International Society on Toxinology
Place of Presentation
University of Oxford, Oxford, UK
Year and Date
2015-09-25
Related Report
Int'l Joint Research
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