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Basic Research for the Evaluation of Antioxidant Activity of Functional Food

Research Project

Project/Area Number 25450178
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionNihon Pharmaceutical University

Principal Investigator

Anzai Kazunori  日本薬科大学, 薬学部, 教授 (70128643)

Co-Investigator(Kenkyū-buntansha) KUBOTA YOKO  日本薬科大学, 薬学部, 准教授 (60271417)
TSUCHIDA KAZUNORI  日本薬科大学, 薬学部, 講師 (50406633)
TAKAJYO TOKUKO  日本薬科大学, 薬学部, 講師 (80424068)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsフェントン反応 / ヒドロキシルラジカル / 2価鉄イオン / ESRスピントラッピング法 / 脂質過酸化 / 溶血 / 抗酸化活性 / スピントラップ法 / ORAC法 / TBA法 / シス-パリナリン酸 / DMPO
Outline of Final Research Achievements

ESR-spin trapping and methods for the measurement of lipid peroxidation were examined for the application to evaluate antioxidant activities of various functional foods. Biphasic dependency of the generation of hydroxyl radical on the Fe2+ concentration was observed when Fenton reaction was used. This finding suggests that effects of functional foods on the concentration of Fe2+ should be considered when Fenton reaction is used to generate hydroxyl radical for the evaluation of hydroxyl radical scavenging activity of the samples. As one of evaluation methods for lipid peroxidation, a technique using hemolysis of red blood cells was examined. The method was improved and various antioxidants were examined by this technique. This method may be useful for the evaluation of lipophilic antioxidants. Results suggesting different roles of Fe2+ on lipid peroxidation were also obtained.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Density of Hydroxyl Radicals Generated in an Aqueous Solution by Irradiating Carbon-Ion Beam2015

    • Author(s)
      松本 謙一郎,上野 恵美,中西 郁夫,安西 和紀
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 63 Issue: 3 Pages: 195-199

    • DOI

      10.1248/cpb.c14-00736

    • NAID

      130004803772

    • ISSN
      0009-2363, 1347-5223
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] YC2014

    • Author(s)
      Yang HL, Huang PJ, Liu YR, Kumar KJ, Hsu LS, Lu TL, Chia YC, Takajo T, Kazunori A, Hseu YC
    • Journal Title

      Oxid Med Cell Longev

      Volume: 2014 Pages: 1-16

    • DOI

      10.1155/2014/901315

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 担がんマウスを用いた炭素イオン線による腫瘍抑制に対する放射線防御剤の及ぼす影響2014

    • Author(s)
      上野恵美、松本謙一郎、安西和紀、髙田二郎
    • Journal Title

      ビタミンE研究の進歩

      Volume: XVI Pages: 19-22

    • Related Report
      2014 Research-status Report
  • [Journal Article] Gamma-tocopherol-N,N-dimethylglycine ester as a potent post-irradiation mitigator against whole body X-irradiation-induced bone marrow death in mice2014

    • Author(s)
      Kazunori Anzai, Megumi Ueno, Ken-ichiro Matsumoto, Nobuo Ikota, Jiro Takata
    • Journal Title

      J. Radiat. Res.

      Volume: 55 Issue: 1 Pages: 67-74

    • DOI

      10.1093/jrr/rrt094

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Scavenging of reactive oxygen species induced by hyperthermia in biological fluid2014

    • Author(s)
      上野恵美、乳井美奈子、中西郁夫、安西和紀、小澤俊彦、松本謙一郎、宇都義浩
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 54 Issue: 2 Pages: 75-80

    • DOI

      10.3164/jcbn.13-61

    • NAID

      130004879349

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lineal energy-based evaluation of oxidative DNA damage induced by proton beams and X-rays2013

    • Author(s)
      Zhengshan Hong, Yuki Kase, Takashi Moritake, Ariungerel Gerelchuluun, Lue Sun, Kenshi Suzuki, Toshiyuki Terunuma, Kiyoshi Yasuoka, Hiroaki Kumada, Kazunori Anzai, Hideyuki Sakurai, Takeji Sakae and Koji Tsuboi
    • Journal Title

      Int. J. Radiat. Biol.

      Volume: Vol.89 Issue: 1 Pages: 36-43

    • DOI

      10.3109/09553002.2012.715791

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of in vivo and in vitro antioxidative parameters for eleven food factors2013

    • Author(s)
      H. P. Indo
    • Journal Title

      RSC Advances

      Volume: 3 Issue: 14 Pages: 4535-4538

    • DOI

      10.1039/c3ra22686g

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 安定ニトロキシルラジカルを主に用いたESR法の生体応用に関する研究2013

    • Author(s)
      安西和紀
    • Journal Title

      電子スピンサイエンス

      Volume: 11 Pages: 4-11

    • Related Report
      2013 Research-status Report
  • [Presentation] Evaluation method for antioxidant activity of various compounds using an azo-compound-induced hemolysis of erythrocytes2015

    • Author(s)
      Nuruki Y., Yokoi A., Takajo T. and Anzai K
    • Organizer
      7th Biennial Meeting of Society for Free Radical Research-Asia (SFRR-Asia 2015)
    • Place of Presentation
      Chiang Mai, Thailand
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 赤血球の溶血を指標とした抗酸化能評価2015

    • Author(s)
      安西和紀、塗木勇介、横井杏沙、高城徳子
    • Organizer
      第68回日本酸化ストレス学会学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] g-TDMG and Zn-yest, radiation mitigators, did not affect the carbon beam-induced growth inhibition og Xenograft tumor on mice2015

    • Author(s)
      Kazunori Anzai, Megumi Ueno, Ken-ichiro Matsumoto, Jiro Takata
    • Organizer
      15th Inteenational Congress of Radiation Resesarch
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フェントン反応によるヒドロキシルラジカル発生系における鉄イオンの影響2015

    • Author(s)
      阿部真也、福間隆元、田口貴将、高城徳子、土田和徳、窪田洋子、安西和紀
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] TEMP (2,2,6,6,-tetramethyl-4-piperidone) を用いた一重項酸素の測定2015

    • Author(s)
      高城徳子、三宅大輔、土田和徳、窪田洋子、安西和紀
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 赤血球の溶血を指標としたビタミンE関連化合物の抗酸化能評価2015

    • Author(s)
      高城徳子、塗木勇介、横井杏沙、安西和紀
    • Organizer
      第26回ビタミンE研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 抗酸化活性尿酸アナログのラット胸腺細胞を用いた放射線防護活性評価2014

    • Author(s)
      関根(鈴木)絵美子、小川幸大、安田大輔、高橋恭子、中西郁夫、上野恵美、松本謙一郎、安西和紀、増野匡彦、村上健
    • Organizer
      第67回日本酸化ストレス学会学術集会
    • Place of Presentation
      京都市
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 放射線被曝後投与でマウスの骨髄死を防御するビタミンE誘導体γ-TDMG2014

    • Author(s)
      安西和紀、上野恵美、松本謙一郎、髙田二郎
    • Organizer
      第17回Vitamin E Update Forum
    • Place of Presentation
      東京
    • Year and Date
      2014-08-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Radioprotective and radiomitigable activity screening of natural antioxidants and the quantitative evaluation2014

    • Author(s)
      Emiko Sekine, Ikuo Nakanishi, Megumi Ueno, Yukihhiro Ogawa, Tsukasa Waki, Takashi Shimokawa, Ken-ichiro Matsumoto, Kazunori Anzai, Takashi Murakami
    • Organizer
      17th Biennial Meeting of Society for Free Radical Research International
    • Place of Presentation
      京都市
    • Related Report
      2013 Research-status Report
  • [Presentation] 放射線による活性酸素生成、生体障害、およびその防御2013

    • Author(s)
      安西和紀
    • Organizer
      第66回日本酸化ストレス学会学術集会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ラット胸腺細部を用いた抗酸化剤のスクリーニング2013

    • Author(s)
      関根(鈴木)絵美子、中西郁夫、上野恵美、下川卓志、松本謙一郎、今井高志、安西和紀、村上健
    • Organizer
      第66回日本酸化ストレス学会学術集会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] ビタミンCおよびその誘導体の非水溶媒中におけるフリーラジカル消去反応2013

    • Author(s)
      中西郁夫、稲見圭子、野村昌吾、和氣司、大久保敬、福原潔、鎌田正、安西和紀、福住俊一、小澤俊彦、松本謙一郎、望月正隆
    • Organizer
      第66回日本酸化ストレス学会学術集会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 活性酸素と防護2013

    • Author(s)
      安西和紀、松本謙一郎、中西郁夫
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      青森市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 被ばく後の投与で有効な放射線障害防護剤2013

    • Author(s)
      安西和紀、上野恵美
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      青森市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 天然抗酸化物質の放射線防護活性スクリーニングとラジカル消去能の定量的評価2013

    • Author(s)
      関根(鈴木)絵美子、中西郁夫、上野恵美、和氣司、小川幸大、下川卓志、松本謙一郎、安西和紀、村上健
    • Organizer
      第52回電子サイエンス学会年会
    • Place of Presentation
      さいたま市
    • Related Report
      2013 Research-status Report
  • [Presentation] 抗参加評価のためのスピントラップ法におけるFenton反応の検討2013

    • Author(s)
      高城徳子、田口貴将、福間隆元、楊新玲、張淑貞、土田和徳、窪田洋子、安西和紀
    • Organizer
      第52回電子サイエンス学会年会
    • Place of Presentation
      さいたま市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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