• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of action mechanism to microbial cells by low pressure carbon dioxide microbubbles

Research Project

Project/Area Number 25450182
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Kobayashi Fumiyuki  日本獣医生命科学大学, 応用生命科学部, 講師 (50460001)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsマイクロ・ナノバブル / 加圧二酸化炭素 / 殺菌 / 酵母 / pH / 細胞膜 / 二酸化炭素 / 細胞内pH / 低加圧二酸化炭素マイクロ・ナノバブル / 細胞膜損傷
Outline of Final Research Achievements

To investigate the effects of intracellular acidification and on cellular membrane in the inactivation of Saccharomyces pastorianus by low pressure carbon dioxide micro-nano bubbles (MNBCO2), intracellular pH, damage and fluidity of cellular membrane, and storage behavior of S. pastorianus treated by MNBCO2 at different conditions were examined. In consequence, it was recognized that MNBCO2 at temperature more than 45℃ had a strong effect on S. pastorianus cells. In addition, inactivation of S. pastorianus by MNBCO2 was responsible primarily for the damage to cellular membrane, although bear no immediate relationship to intracellular acidification.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (5 results)

  • [Journal Article] Comparison of a two-stage system with low pressure carbon dioxide microbubbles and heat treatment on the inactivation of Saccharomyces pastorianus cells2014

    • Author(s)
      Fumiyuki Kobayashi, Masaki Sugiura, Hiromi Ikeura, Michio Sato, Sachiko Odake, Masahiko Tamaki
    • Journal Title

      Food Control

      Volume: 46 Pages: 35-40

    • DOI

      10.1016/j.foodcont.2014.04.034

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quality evaluation of sake treated with a two-stage system of low pressure carbon dioxide microbubbles2014

    • Author(s)
      Fumiyuki Kobayashi, Hiromi Ikeura, Sachiko Odake, Hiroshi Sakurai
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 62 Issue: 48 Pages: 11722-11729

    • DOI

      10.1021/jf5038618

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inactivation of Saccharomyces cerevisiae by equipment pressurizing at ambient temperature after generating CO2 microbubbles at lower temperature and pressure2013

    • Author(s)
      Fumiyuki Kobayashi, Hiromi Ikeura, Sachiko Odake, Yasuyoshi Hayata
    • Journal Title

      LWT - Food Science and Technology

      Volume: 56 Issue: 2 Pages: 543-547

    • DOI

      10.1016/j.lwt.2013.11.022

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inactivation of Fusarium oxysporum f.sp. melonis and Pectobacterium carotovorum subsp. carotovorum in hydroponic nutrient solution by low-pressure carbon dioxide microbubbles2013

    • Author(s)
      Fumiyuki Kobayashi, Masaki Sugiura, Hiromi Ikeura, Kanami Sato, Sachiko Odake, Yasuyoshi Hayata
    • Journal Title

      Scientia Horticulturae

      Volume: 164 Pages: 596-601

    • DOI

      10.1016/j.scienta.2013.10.021

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] マイクロバブル化した加圧二酸化炭素による食品の殺菌・酵素失活に関する研究2013

    • Author(s)
      小林史幸
    • Journal Title

      日本獣医生命科学大学研究報告

      Volume: 62 Pages: 64-67

    • Related Report
      2013 Research-status Report
  • [Presentation] 低加圧二酸化炭素マイクロバブルによるSaccharomyces pastorianusの殺菌における細胞内酸性化および細胞膜損傷の影響2015

    • Author(s)
      小林史幸、小竹佐知子
    • Organizer
      日本農芸化学会関東支部大会
    • Place of Presentation
      お茶の水女子大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低加圧二酸化炭素マイクロバブル処理による清酒の殺菌・酵素失活について2015

    • Author(s)
      小林史幸、池浦博美、小竹佐知子、桜井博志
    • Organizer
      農芸化学会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 二酸化炭素マイクロバブル処理した清酒の品質評価2014

    • Author(s)
      小林史幸、池浦博美、小竹佐知子、桜井博志
    • Organizer
      日本マイクロ・ナノバブル学会
    • Place of Presentation
      東京
    • Year and Date
      2014-12-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 低加圧二酸化炭素マイクロバブルによるビールの殺菌2013

    • Author(s)
      小林史幸・杉浦匡紀・池浦博美・小竹佐知子・玉置雅彦
    • Organizer
      日本食品工学会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 二酸化炭素マイクロ・ナノバブルによるビール酵母の殺菌2013

    • Author(s)
      小林史幸・杉浦匡紀・池浦博美・佐藤道夫・小竹佐知子・玉置雅彦
    • Organizer
      日本マイクロ・ナノバブル学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi