| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Tri-and tetra-motif repeat microsatellite marker loci were developed for the white-spotted charr (Salvelinus leucomaenis). The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite-primer sets, available for the estimation of polymorphisms, were identified. To evaluate individual reproductive success of white-spotted charr, I collected nine consecutive years DNA samples of white-spotted charr in a wild population. Both males and females exhibited greater variances in reproductive success among individuals, 0-29% (min.-max. during 2005-2013) of the mature males were assigned fatherhood of the offspring, and 9-100% of the mature females were assigned motherhood of the offspring (full-pedigree likelihood method). I also examined reproductive success of hatchery-reared white-spotted charr in a semi-natural artificial stream. Hatchery-reared fish also exhibited a large individual variation in reproductive success in both males and females.
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