| Project/Area Number |
25450391
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Yusuke 宇都宮大学, 農学部, 助教 (50589520)
FUKUMORI Rika 宇都宮大学, 農学部, 助教 (60721694)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 内因性ペプチド / 摂食調節ペプチド / 自発的飼料摂取量 / 摂食量 / 脳微小透析法 / 側脳室 / 日内変動 / 時間分解蛍光免疫測定法 / 自発的摂食量 / ニワトリ / NPY / GLP-1 / in vitro 回収率 / ペプチドダイアリシス / 鶏ヒナ |
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| Outline of Final Research Achievements |
This study was designed to examine if the endogenous GLP-1 and NPY in the brain was associated with the diurnal food intake variation in growing chickens using the brain microdialysis technique. In vitro recovery ratio was as much as 3% for the both peptides. GLP-1 and NPY in dialysate from the lateral ventricle through the microdialysis probe (polyethylene, MWCO 1000 kDa) was measured in commercial ELISA kits for chickens. The concentration of GLP-1 and NPY was 100 to 300 pg/mL and less than 10 pg/mL, respectively. The variation of voluntary food intake was observed in chickens implanted cannula, but it did not follow concomitant variation of any peptides. Time-resolved fluoroimmunoassay for GLP-1 in the dialysate failed to get quantitative values. It is unlikely that GLP-1 or NPY involved in regulation of diurnal variation of food intake.
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