Isolation and genome analysis of novel methanogens from the rumen.

• Project/Area Number: 25450393
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Mie University
• Principal Investigator: MATSUI Hiroki 三重大学, 生物資源学研究科, 教授 (30346001)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: メタン生成菌 / ルーメン / メタン生成抑制 / ゲノム解析 / インビトロ培養 / 反芻家畜 / 嫌気性真菌

Outline of Final Research Achievements

Livestock cattle is considered to contribute 15% of total methane emission. Methanogens produce methane in the rumen of cattle. Therefore, characterization of methanogens is required. In this project, a new isolation method which uses symbiotic microorganisms was developed and isolates were collected and characterized. Furthermore, genome sequence of one isolates from the collection was analyze.

Research Products

• [Journal Article] Changes in in vitro rumen fermentation characteristics of different compositions of total mixed rations TMR and the ensiled TMRs (2016)
  • Author(s): Wang, C., Y. Ueno,* A. Jayanegara, M. Kondo, T. Ban-Tokuda, H. Matsui.
  • Journal Title: Advances in Animal and Veterinary Sciences. Volume: 4 Issue: 4 Pages: 178-182
  • DOI: 10.14737/journal.aavs/2016/4.4.178.182
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Increase in rumen fibrolytic bacteria and the improvement of fiber degradability of ensiling total mixed ration assessed by in vitro rumen culture. (2016)
  • Author(s): Uyeno, Y., C. Wang, A. Jayanegara, M. Kondo, T. Ban-Tokuda, H. Matsui.
  • Journal Title: Advances in Animal and Veterinary Sciences. Volume: 4 Issue: 4 Pages: 183-186
  • DOI: 10.14737/journal.aavs/2016/4.4.183.186
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of supplementation of rice bran and fumarate alone or in combination on in vitro rumen fermentation, methanogenesis, and methanogens. (2016)
  • Author(s): Arfan Abrar, M. Kondo, T. Kitamura, T. Ban-Tokuda, and H. Matsui.
  • Journal Title: Animal Science Journal Volume: 87 Issue: 3 Pages: 398-404
  • DOI: 10.1111/asj.12431
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The effect of dietary calcium salt medium-chain fatty acid on rumen fermentation in vitro, methanogenesis and rumen microbial density (2016)
  • Author(s): Arfan Abrar, M. Kondo, T. Kitamura, T. Ban-Tokuda, and H. Matsui.
  • Journal Title: Pakistan Journal of Nutrition Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Diversity and fluctuation in ciliate protozoan population in the rumen of cattle. (2016)
  • Author(s): Arfan Abrar, H. Watanabe, T. Kitamura, M. Kondo, T. Ban-Tokuda, H. Matsui
  • Journal Title: Animal Science Journal. Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of monensin withdrawal on rumen fermentation, methanogenesis and microbial populations in cattle. (2015)
  • Author(s): Arfan Abrar, T. Tsukahara, M. Kondo, T. Ban-Tokuda, C. Wang, and H. Matsui.
  • Journal Title: Animal Science Journal Volume: 86 Issue: 9 Pages: 849-854
  • DOI: 10.1111/asj.12368
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of bromochloromethane and fumarate on phylogenetic diversity of the formyltetrahydrofolate synthetase gene in the bovine rumen. (2014)
  • Author(s): Mitsumori, M., H. Matsui, K. Tajima, T. Shinkai, A. Takenaka, S. Denman, and C. McSweeney.
  • Journal Title: Animal Science Journal Volume: 85 Issue: 1 Pages: 25-31
  • DOI: 10.1111/asj.12072
  • Peer Reviewed
• [Journal Article] Comparative analysis of the methanogen diversity in horse and pony by using mcrA gene and archaeal 16S rRNA gene clone libraries. (2014)
  • Author(s): Lwin, K. O., and H. Matsui.
  • Journal Title: Archaea Volume: 2014 Pages: 483574-483574
  • DOI: 10.1155/2014/483574
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Effect of fumarate and rice bran supplementation on in vitro rumen fermentation and methanogenesis. (2014)
  • Author(s): Abrar A, Kondo M, Ban-Tokuda T, Matsui H.
  • Organizer: the 16th AAAP Animal Science Congress
  • Place of Presentation: ガジャマダ大学、ジョクジャカルタ、インドネシア
  • Year and Date: 2014-10-10 – 2014-10-14
• [Presentation] Supplementation of fumarate and rice bran on in vitro fermentation and methanogenesis (2014)
  • Author(s): ArfanAbrar, 伴（德田）智美, 近藤誠, 松井宏樹
  • Organizer: 第118回日本畜産学会
  • Place of Presentation: エポカルつくば 茨城県つくば市