Determination of bovine leukemia virus susceptible and resistant gene by the comparison of Japanese native cattle with Japanese Black cattle
Project/Area Number |
25450405
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Animal production science
|
Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Takeshima Shin-nosuke 国立研究開発法人理化学研究所, 分子ウイルス学特別研究ユニット, 研究員 (60342812)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 日本在来牛 / ウシ主要組織適合遺伝子複合体(BoLA) / 疾患感受性 / ゲノムリシークエンス / 牛白血病ウイルス / 口之島牛 / 次世代シークエンサー / 見島牛 / ウシ主要組織適合遺伝子複合体(BoLA) / CNTN3 / 主成分分析 / 牛白血病 / 主要組織適合抗原 / ターゲットリシークエンス / ウシゲノム / リシークエンス / 主要組織適合遺伝子複合体 |
Outline of Final Research Achievements |
We sequenced bovine major histocompatibility complex region for 12 of Kuchinoshima cattle, 11 of Mishima cattle and 10 Japanese black cattle by target re-sequencing method using Next generation sequencer to determine whether the susceptible/resistant genotype for bovine leukemia virus were originated from Japanese native cattle or not. PCA analysis showed that susceptible gene were came from Japanese native cattle but resistant gene were introduced from foreign country.
|
Report
(4 results)
Research Products
(13 results)
-
-
[Journal Article] A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis.2016
Author(s)
M Polat,# S-n Takeshima,# K Hosomichi, J Kim, T Miyasaka, K Yamada, M Arainga, T Murakami, Y Matsumoto, V de la Barra Diaz, CJ Panei, ET Gonzalez, M Kanemaki, M Onuma, G Giovambattista, and Y Aida. #,equal contribution
-
Journal Title
Retrovirology
Volume: 13(4)
Issue: 1
Pages: 4-4
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
[Journal Article] Assessment of biodiversity in Chilean cattle using the distribution of major histocompatibility complex class II BoLA-DRB3 allele.2015
Author(s)
Takeshima SN, Miyasaka T, Matsumoto Y, Xue G, Diaz Vde L, Rogberg-Muñoz A, Giovambattista G, Ortiz M, Oltra J, Kanemaki M, Onuma M, Aida Y.
-
Journal Title
Tissue Antigens
Volume: 85
Issue: 1
Pages: 35-44
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] The great diversity of major histocompatibility complex class II genes in Philippine native cattle2014
Author(s)
Takeshima, S.N., Miyasaka, T., Polat, M., Kikuya, M., Matsumoto, Y., Mingala, C.N., Villanueva, M.A., Salces, A.J., Onuma, M. and Aida, Y
-
Journal Title
Meta Gene
Volume: 2
Pages: 176-190
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle2014
Author(s)
Goszczynski, D.E., Ripoli, M.V., Takeshima, S.N., Baltian, L., Aida, Y. and Giovambattista, G
-
Journal Title
Tissue Antigens
Volume: 83
Issue: 3
Pages: 180-183
DOI
Related Report
Peer Reviewed
-
-
-