Determination of bovine leukemia virus susceptible and resistant gene by the comparison of Japanese native cattle with Japanese Black cattle

• Project/Area Number: 25450405
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Takeshima Shin-nosuke 国立研究開発法人理化学研究所, 分子ウイルス学特別研究ユニット, 研究員 (60342812)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 日本在来牛 / ウシ主要組織適合遺伝子複合体（BoLA) / 疾患感受性 / ゲノムリシークエンス / 牛白血病ウイルス / 口之島牛 / 次世代シークエンサー / 見島牛 / ウシ主要組織適合遺伝子複合体(BoLA) / CNTN3 / 主成分分析 / 牛白血病 / 主要組織適合抗原 / ターゲットリシークエンス / ウシゲノム / リシークエンス / 主要組織適合遺伝子複合体

Outline of Final Research Achievements

We sequenced bovine major histocompatibility complex region for 12 of Kuchinoshima cattle, 11 of Mishima cattle and 10 Japanese black cattle by target re-sequencing method using Next generation sequencer to determine whether the susceptible/resistant genotype for bovine leukemia virus were originated from Japanese native cattle or not. PCA analysis showed that susceptible gene were came from Japanese native cattle but resistant gene were introduced from foreign country.

Research Products

• [Journal Article] Development of a direct blood-based PCR system to detect BLV provirus using CoCoMo primers (2016)
  • Author(s): Shin-nosuke Takeshima, Sonoko Watanuki, Hiroshi Ishizaki, Kazuhiro Matoba, Yoko Aida
  • Journal Title: Archives of Virology Volume: 印刷中 Issue: 6 Pages: 1539-1546
  • DOI: 10.1007/s00705-016-2806-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis. (2016)
  • Author(s): M Polat,# S-n Takeshima,# K Hosomichi, J Kim, T Miyasaka, K Yamada, M Arainga, T Murakami, Y Matsumoto, V de la Barra Diaz, CJ Panei, ET Gonzalez, M Kanemaki, M Onuma, G Giovambattista, and Y Aida. #,equal contribution
  • Journal Title: Retrovirology Volume: 13(4) Issue: 1 Pages: 4-4
  • DOI: 10.1186/s12977-016-0239-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The diversity of major histocompatibility complex class II DRB1 gene in sheep breeds from Xinjiang, China (2015)
  • Author(s): M. Polat, Y. Aida, S.-N. Takeshima, J. Aniwashi & M. Halik
  • Journal Title: Tissue Antigens Volume: 85 Issue: 1 Pages: 50-57
  • DOI: 10.1111/tan.12480
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of bovine MHC class II DRB3 diversity in South American Holstein cattle populations. (2015)
  • Author(s): S.-N. Takeshima#, G. Giovambattista#, N. Okimoto, Y. Matsumoto, A. Rogberg-Munoz, T. J. Acosta, M. Onuma and Y. Aida,#,equal contribution
  • Journal Title: Tissue Antigens Volume: 86(6) Issue: 6 Pages: 419-430
  • DOI: 10.1111/tan.12692
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Detection and molecular characterization of bovine leukemia virus in Philippine cattle. (2015)
  • Author(s): Polat M, Ohno A, Takeshima SN, Kim J, Kikuya M, Matsumoto Y, Mingala CN, Onuma M, Aida Y.
  • Journal Title: Arch Virol. Volume: 160 Issue: 1 Pages: 285-296
  • DOI: 10.1007/s00705-014-2280-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Risk factors associated with increased bovine leukemia virus proviralload in infected cattle in Japan from 2012 to 2014 (2015)
  • Author(s): Ayumu Ohno, Shin-nosuke Takeshima, Yuki Matsumoto, Yoko Aida
  • Journal Title: Virus Research Volume: 210 Pages: 283-290
  • DOI: 10.1016/j.virusres.2015.08.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve. (2015)
  • Author(s): Takeshima SN, Kitamura-Muramatsu Y, Yuan Y, Polat M, Saito S, Aida Y.
  • Journal Title: Arch Virol. Volume: 160 Issue: 5 Pages: 1325-1332
  • DOI: 10.1007/s00705-015-2377-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Assessment of biodiversity in Chilean cattle using the distribution of major histocompatibility complex class II BoLA-DRB3 allele. (2015)
  • Author(s): Takeshima SN, Miyasaka T, Matsumoto Y, Xue G, Diaz Vde L, Rogberg-Muñoz A, Giovambattista G, Ortiz M, Oltra J, Kanemaki M, Onuma M, Aida Y.
  • Journal Title: Tissue Antigens Volume: 85 Issue: 1 Pages: 35-44
  • DOI: 10.1111/tan.12481
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The great diversity of major histocompatibility complex class II genes in Philippine native cattle (2014)
  • Author(s): Takeshima, S.N., Miyasaka, T., Polat, M., Kikuya, M., Matsumoto, Y., Mingala, C.N., Villanueva, M.A., Salces, A.J., Onuma, M. and Aida, Y
  • Journal Title: Meta Gene Volume: 2 Pages: 176-190
  • DOI: 10.1016/j.mgene.2013.12.005
  • Peer Reviewed / Open Access
• [Journal Article] Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle (2014)
  • Author(s): Goszczynski, D.E., Ripoli, M.V., Takeshima, S.N., Baltian, L., Aida, Y. and Giovambattista, G
  • Journal Title: Tissue Antigens Volume: 83 Issue: 3 Pages: 180-183
  • DOI: 10.1111/tan.12293
  • Peer Reviewed
• [Journal Article] Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus. (2013)
  • Author(s): Aida Y, Murakami H, Takahashi M, Takeshima SN.
  • Journal Title: Front Microbiol Volume: 328 Pages: 1-11
  • DOI: 10.3389/fmicb.2013.00328
  • Peer Reviewed
• [Presentation] 口之島牛のウシ主要組織適合遺伝子複合体領域（MHC)領域のゲノム多様性 (2014)
  • Author(s): 竹嶋伸之輔、細道一善、菊谷真里、Meripet Polat, 下桐猛、印牧美佐夫、萬年英之、国枝哲夫、間陽子
  • Organizer: 動物遺伝育種学会第15回大会
  • Place of Presentation: 埼玉県和光市
  • Year and Date: 2014-10-30 – 2014-11-01
• [Book] 獣医免疫学 (2015)
  • Author(s): 竹嶋伸之輔・間陽子
  • Publisher: 緑書房