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Analyses of energy metabolism adapted to a low oxygen environment in developmental stages of Eimeria

Research Project

Project/Area Number 25450436
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionOsaka Prefecture University (2015-2016)
National Agriculture and Food Research Organization (2013-2014)

Principal Investigator

Matsubayashi Makoto  大阪府立大学, 生命環境科学研究科, 准教授 (00321076)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsコクシジウム / 呼吸鎖 / 低酸素 / ミトコンドリア / Eimeria tenella / 鶏 / エネルギー代謝 / RNA sequencing / real-time PCR
Outline of Final Research Achievements

Control of chicken coccidia is important issue in poultry industry. The object of this study is focused on producing the safe and new anti-coccidiostat. Consequently, we successfully constructed the methods of purification of Eimeria tenella mitochondria and assay system of the mitochondria activity. Using the systems, we could found some compounds could inhibit the activities in vitro. Furthermore, these compounds could kill the purified sporozoites and merozoites. These results can show utility for clarifying the respiratory chain activity of Eimeria spp. and finding seed compounds against the parasites.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (38 results)

All 2017 2016 2015 2014 2013

All Journal Article (25 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 24 results,  Open Access: 18 results,  Acknowledgement Compliant: 15 results) Presentation (13 results)

  • [Journal Article] Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves2017

    • Author(s)
      Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai.
    • Journal Title

      Acta Parasitologica

      Volume: 62(1) Issue: 1 Pages: 214-220

    • DOI

      10.1515/ap-2017-0026

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody2016

    • Author(s)
      Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Hyun S. Lillehoj, Haruo Matsuda, Shigeo Takenaka, Takeshi Hatta, Naotoshi Tsuji, Kazumi Sasai.
    • Journal Title

      Parasitology Research

      Volume: 115(11) Pages: 4123-4128

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Transcriptional profiles of virulent and precocious strains of Eimeria tenella at sporozoite stage; novel biological insight into attenuated asexual development2016

    • Author(s)
      Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Junya Yamagishi, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 40 Pages: 54-62

    • DOI

      10.1016/j.meegid.2016.02.021

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] First report of molecular identification of Cystoisospora suis in a piglet with lethal diarrhea in Japan2016

    • Author(s)
      Makoto Matsubayashi, Hideko Takayama, Masahiro Kusumoto, Misato Murata, Yuka Uchiyama, Masaya Kaji, Kazumi Sasai, Ryosaku Yamaguchi, Tomoyuki Shibahara
    • Journal Title

      Acta Parasitologica

      Volume: 61(2) Issue: 2 Pages: 406-11

    • DOI

      10.1515/ap-2016-0054

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice2016

    • Author(s)
      Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo
    • Journal Title

      Acta Parasitologica

      Volume: 115(1) Issue: 1 Pages: 211-5

    • DOI

      10.1007/s00436-015-4737-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A serological survey of antobodied against Leptospira spp. from wild raccoons (Procyon lotor) in Osaka, Japan2016

    • Author(s)
      Saeki Jun, Nakanishi Hitoshi, Masubuchi Katsuo, Matsubayashi Makoto, Furuya Masaru, Tani Hiroyuki, Sasai Kazumi
    • Journal Title

      Asian Journal of Animal and Veterinary Advances

      Volume: 未定

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic identification of Entamoeba polecki subtype 3 from pigs in Japan and characterisation of its pathogenic role in ulcerative colitis2015

    • Author(s)
      Makoto Matsubayashi, Naoko Murakoshi, Tetsuya Komatsu, Masaharu Tokoro, Makoto Haritani, Tomoyuki Shibahara
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 36 Pages: 8-14

    • DOI

      10.1016/j.meegid.2015.08.030

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Localization of heat shock protein 110 in canine mammary gland tumor2015

    • Author(s)
      Satoru Okada, Masaru Furuya, Shigeo Takenaka, Ayano Fukui, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: 167(3-4) Issue: 3-4 Pages: 139-46

    • DOI

      10.1016/j.vetimm.2015.07.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Onchocerca takaokai n. sp. (Nematoda: Filarioidea) in Japanese wild boars (Sus scrofa leucomystax): Description and molecular identification of intradermal females2015

    • Author(s)
      Shigehiko Uni, Masako Fukuda, Takeshi Agatsuma, Odile Bain, Yasushi Otsuka, Jun Nakatani, Makoto Matsubayashi, Masashi Harada, Hasmahzaiti Omar, Rosli Ramli, Rosli Hashim, Mohd Sofian Azirun, Hiroyuki Takaoka
    • Journal Title

      Parasitology International

      Volume: 64 Issue: 6 Pages: 493-502

    • DOI

      10.1016/j.parint.2015.07.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] First molecular identification of Entamoeba polecki in a piglet in Japan and implications for aggravation of ileitis by coinfection with Lawsonia intracellularis2015

    • Author(s)
      Makoto Matsubayashi, Kenta Kanamori, Masayuki Sadahiro, Masaharu Tokoro, Niichiro Abe, Makoto Haritani, Tomoyuki Shibahara
    • Journal Title

      Parasitology Research

      Volume: 114(8) Issue: 8 Pages: 3069-73

    • DOI

      10.1007/s00436-015-4509-9

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The detection of toxigenic Corynebacterium ulcerans from cats with nasal inflammation in Japan.2015

    • Author(s)
      Saeki J, Katsukawa C, Matsubayashi M, Nakanishi H, Furuya M, Tani H, Sasai K.
    • Journal Title

      Epidemiol Infect.

      Volume: 1 Issue: 12 Pages: 1-6

    • DOI

      10.1017/s0950268814003550

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 豚の消化器疾患におけるEntamoeba poleckiとEntamoeba suisの病原性2015

    • Author(s)
      金森健太, 貞弘真行, 松林 誠, 所 正治, 阿部 仁一郎, 播谷 亮, 芝原友幸
    • Journal Title

      日本豚病研究会報

      Volume: 67 Pages: 28-33

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 離乳後問題となる疾病2015

    • Author(s)
      芝原友幸, 宮﨑綾子, 生澤充隆, 山口遼作, 松林 誠
    • Journal Title

      日本豚病研究会報

      Volume: 66 Pages: 22-25

    • NAID

      50009481708

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] パラフィン包埋切片を用いた種特異的PCRによる子牛の出血性直腸炎におけるコクシジウムの遺伝子学的同定2015

    • Author(s)
      是枝輝紀,野尻麻里子,堂下さつき,松林 誠,芝原友幸,岡野良一
    • Journal Title

      日本獣医師会雑誌

      Volume: 68 Pages: 297-300

    • NAID

      130005078180

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 牛のクリプトスポリジウム下痢症 「牛のクリプトスポリジウム症」2015

    • Author(s)
      松林 誠
    • Journal Title

      臨床獣医

      Volume: 33(3) Pages: 12-15

    • Related Report
      2014 Research-status Report
  • [Journal Article] 豚のアメーバ症;出血性大腸炎の豚から同定されたEntamoeba suis2015

    • Author(s)
      鈴田史子、松林 誠、寺山好美、下條憲吾、油井 武、播谷 亮、芝原友幸
    • Journal Title

      日本豚病研究会報

      Volume: 65 Pages: 44-50

    • NAID

      50009252442

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents2015

    • Author(s)
      Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Masashi Tsujio, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Kozo Fujisaki, Toshihiro Matsui and Tomohide Matsuo
    • Journal Title

      Acta Parasitologica

      Volume: 60(2) Pages: 190-195

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DB-AT: a 2015 update to the Full-parasites database brings a multitude of new transcriptomic data for apicomplexan parasites2014

    • Author(s)
      Jakalski, M, Wakaguri H, Kischka T, Nishikawa Y, Kawazu S, Matsubayashi M, Kawahara F, Tsuji N, Cao S, Sunaga F, Xuan X, Okubo K, Igarashi I, Tuda J, Mongan A, Eshita Y, Maeda R, Makalowski W, Suzuki Y, Yamagishi J
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Issue: D1 Pages: D631-D636

    • DOI

      10.1093/nar/gku1240

    • NAID

      120005602520

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Unusual ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity2014

    • Author(s)
      Makoto Matsubayashi, Fumiko Suzuta, Yoshimi Terayama, Kengo Shimojo, Takeshi Yui, Makoto Haritani, Tomoyuki Shibahara
    • Journal Title

      Parasitology Research

      Volume: 113(8) Issue: 8 Pages: 3023-8

    • DOI

      10.1007/s00436-014-3965-y

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic analysis of Enterobius vermicularis isolated from a chimpanzee with lethal hemorrhagic colitis and pathology of the associated lesions2014

    • Author(s)
      Yuji Yaguchi, Sachi Okabayashi, Niichiro Abe, Haruhisa Masatou, Shinya Iida, Isao Teramoto, Makoto Matsubayashi, Tomoyuki Shibahara
    • Journal Title

      Parasitology Research

      Volume: 113(11) Issue: 11 Pages: 4105-9

    • DOI

      10.1007/s00436-014-4080-9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre-and post-weaned piglets and adult pigs in Japan.2014

    • Author(s)
      Takeshi Yui, Toshiyuki Nakajima, Norishige Yamamoto, Marina Kon, Niichiro Abe, Makoto Matsubayashi, Tomoyuki Shibahara.
    • Journal Title

      Parasitology Research

      Volume: 113(1) Issue: 1 Pages: 359-65

    • DOI

      10.1007/s00436-013-3662-2

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Survey on gastrointestinal parasites and detection of Cryptosporidium spp. on cattle in West Java, Indonesia2014

    • Author(s)
      Sylvia Maharani Ananta, Suharno, Adi Hidayat, Makoto Matsubayashi.
    • Journal Title

      Asian Pacific Journal of Tropical Medicine.

      Volume: 7(3) Issue: 3 Pages: 197-201

    • DOI

      10.1016/s1995-7645(14)60020-1

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Localization of Eimeripain, an <i>Eimeria tenella</i> Cathepsin B-Like Cysteine Protease, during Asexual and Sexual Intracellular Development in Chicken Ceca2014

    • Author(s)
      Makoto Matsubayashi, Takeshi Hatta, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Kayoko Yamaji, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Issue: 4 Pages: 531-537

    • DOI

      10.1292/jvms.13-0509

    • NAID

      130003382415

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Elongation factor-1α is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum.2013

    • Author(s)
      Matsubayashi M, Teramoto-Kimata I, Uni S, Lillehoj HS, Matsuda H, Furuya M, Tani H, Sasai K.
    • Journal Title

      J Biol Chem.

      Volume: 288(47) Issue: 47 Pages: 34111-34120

    • DOI

      10.1074/jbc.m113.515544

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] High-throughput RNA sequencing profiles and transcriptional evidence of aerobic respiratory enzymes in sporulating oocysts and sporozoites of Eimeria tenella.2013

    • Author(s)
      Makoto Matsubayashi, Takeshi Hatta, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji.
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 18 Pages: 269-76

    • DOI

      10.1016/j.meegid.2013.06.002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Cryptosporidium parvum における呼吸鎖関連酵素の特性解析2017

    • Author(s)
      髙橋 和瑛、松林 誠、稲岡 ダニエル 健、八田 岳士、寺本 勲、 金子 明、所 正治、古家 優、谷 浩行、辻 尚利、笹井 和美、北 潔
    • Organizer
      第86回日本寄生虫学会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2017-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Cryptosporidium parvum における呼吸鎖関連酵素の特性解析2016

    • Author(s)
      高橋 和瑛、松林 誠、稲岡 ダニエル 健、八田 岳士、寺本 勲、金子 明、所 正治、古家 優、谷 浩行、辻 尚利、笹井 和美、北 潔.
    • Organizer
      第157回日本獣医学会
    • Place of Presentation
      日本大学(神奈川)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 幼雛におけるEimeria tenella弱毒株の作出および病理組織学的解析2016

    • Author(s)
      松林 誠、川原 史也、八田 岳士、山岸 潤也、磯部 尚、寺本 勲、金子 明、笹井 和美、北 潔、辻 尚利
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 豚の大腸から見出されたEntamoeba属原虫の種および遺伝子型の同定と病理組織学的解析2016

    • Author(s)
      芝原 友幸、鈴田 史子、金森 健太、村越 奈穂子、小松 徹也、松林 誠、阿部 仁一郎、播谷 亮、所 正治
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 抗鶏コクシジウム症薬の開発を目指したEimeria tenella Dihydroorotate dehydrogenaseの発現・精製と結晶化2015

    • Author(s)
      長濱 まどか、佐藤 暖、志波 智生、松林 誠、 稲岡 ダニエル健、辻 尚利、北 潔、原田 繁春
    • Organizer
      第88回日本生化学会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Eimeria tenella のミトコンドリア呼吸鎖酵素活性の測定と阻害剤による虫体殺滅効果2015

    • Author(s)
      松林 誠、稲岡 ダニエル 健、小松谷 啓介、八田 岳士、三好 猛晴、磯部 尚、川原 史也、山岸 潤也、彦坂 健児、佐藤 暖、志波 智生、原田 繁春、北 潔、辻 尚利
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella2014

    • Author(s)
      Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Takeharu Miyoshi, Junya Yamagishi, Anisuzzaman, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Akira Iwata, Naotoshi Tsuji
    • Organizer
      17th Japanese-German Cooperative Symposium on Protozoan Disease
    • Place of Presentation
      Dresden University of Technology (Dresden, Germany)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella2014

    • Author(s)
      Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Takeharu Miyoshi, Junya Yamagishi, Anisuzzaman, Kameo Shimura, Takashi Isobe, Kiyoshi Kita, Akira Iwata, Naotoshi Tsuji
    • Organizer
      11th International Coccidiosis Conference
    • Place of Presentation
      Dresden University of Technology (Dresden, Germany)
    • Year and Date
      2014-09-26 – 2014-09-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Eimeria tenella スポロゾイトのミトコンドリア精製法の確立と呼吸鎖酵素活性2014

    • Author(s)
      松林 誠、稲岡  ダニエル 健、小松谷 啓介、八田 岳士、三好 猛晴、磯部 尚、川原 史也、山岸 潤也、彦坂 健児、佐藤 暖、志波 智生、原田 繁春、北 潔、辻 尚利
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察2014

    • Author(s)
      小野 結菜、松林 誠、川口 博明、辻尾 祐志、田仲 哲也、三好 宣彰、松井 利博、松尾 智英
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 次世代シークエンサーを用いたEimeria tenella 強毒株と弱毒株スポロゾイトの遺伝子発現プロファイリング2014

    • Author(s)
      松林 誠、川原 史也、八田 岳士、三好 猛晴、山岸 潤也、Anisuzzaman M、磯部 尚、北 潔、岩田 晃、辻 尚利.
    • Organizer
      第83回日本寄生虫学会大会学術集会
    • Place of Presentation
      愛媛大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Eimeria tenella 強毒株と弱毒株スポロゾイトの比較トランスクリプトーム解析. 、238.2013

    • Author(s)
      松林 誠、川原 史也、八田 岳士、三好 猛晴、山岸 潤也、M  アニスザマン、志村 亀夫、磯部 尚、岩田 晃、辻 尚利.
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Eimeria tenella 強毒株と弱毒株スポロゾイトの網羅的遺伝子発現比較解析2013

    • Author(s)
      松林 誠、川原史也、八田岳士、三好猛晴、山岸潤也、アニスザマン、志村亀夫、磯部 尚、北潔、岩田 晃、辻 尚利.
    • Organizer
      第11回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎大学
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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