Differentiation mechanism of type ID myofiber as a ruminant-specific super fatigue-resistant myofiber type

• Project/Area Number: 25450461
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tohoku University
• Principal Investigator: WATANABE Kouichi 東北大学, (連合)農学研究科(研究院), 助教 (80261494)
• Co-Investigator(Kenkyū-buntansha): ASO Hisashi 東北大学, 大学院農学研究科, 教授 (50241625)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 超疲労耐久性筋線維 / ウシ / ３－ヒドロキシ酪酸脱水素酵素 / カルシウムイオン関連因子 / Sirtuin / カルニチンアシル基転移酵素ー１ｂ / 機能適応 / ID型筋線維 / 骨格筋 / 筋線維型 / カルニチンアシル基転移酵素-1ｂ / 3-ヒドロキシ酪酸脱水素酵素

Outline of Final Research Achievements

Ruminant-specific type ID myofibers as a super fatigue-resistant myofiber, which are distributed in anti-gravity muscles involved in posture maintenance, possess strong 3-hydroxybutyrate dehydrogenase activity. In bovine muscles, our results suggest that type ID myofibers are possible to keep higher sarcoplasmic calcium ion level for long-term muscular movement, and have higher metabolic capacity of fatty acid than other myofiber types for sufficient energy supply. Moreover, it suggests that sirtuin1 activate PGC-1alpha for increasing of mitochondria in myofiber transition of "type IIB to type IIA" as oxidative change. Successively, upregulation of sirtuin3 in mitochondria seems to lead induction of fast-to-slow transformation and appearance of type ID myofiber involved in super fatigue-resistance.

Research Products

• [Journal Article] 筋線維型と食肉のおいしさ ―反芻動物特異的Ⅰ型筋線維の産肉論的機能形態学解析― (2016)
  • Author(s): 渡邊康一
  • Journal Title: 東北畜産学会報 Volume: 66
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Serotonin improves high fat diet induced obesity in mice. (2016)
  • Author(s): Watanabe H.†, Nakano T.†, Saito R.†, Akasaka D., Saito K., Ogasawara H., Minashima T., Miyazawa K., Kanaya T., Takakura I., Inoue N., Ikeda I., Chen X., Miyake M., Kitazawa H., Shirakawa H., Sato K., Tahara K., Nagasawa Y., Rose M.T., Ohwada S., Watanabe K., Aso H.
  • Journal Title: PLoS One Volume: 11 Issue: 1 Pages: 1-14
  • DOI: 10.1371/journal.pone.0147143
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The regulation of oxytocin receptor gene expression during adipogenesis (2015)
  • Author(s): Yi KJ, So KH, Hata Y, Suzuki Y, Kato D, Watanabe K, Aso H, Kasahara Y, Nishimori K, Chen C, Katoh K, Roh SG
  • Journal Title: J Neuroendocrinology Volume: 27 Issue: 5 Pages: 335-342
  • DOI: 10.1111/jne.12268
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The stimulatory effect of plasma samples from fattening cattle on adipogenesis related gene expression in preadipocyte cells (2015)
  • Author(s): Hitoshi WATANABE, Xiangning CHEN, Noriaki SHOJI, Ryo SAITO, Tatsuya NAKANO, Kazuki SAITO, Keisuke SUMIYOSHI, Michael T. ROSE, Natumi OKADA, Kouichi WATANABE and Hisashi ASO
  • Journal Title: Animal Science Journal Volume: 86 Issue: 7 Pages: 698-706
  • DOI: 10.1111/asj.12344
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double muscled (DM) and normal muscled (NM) Japanese shorthorn cattle. (2014)
  • Author(s): H. Takahashi, K. Sato, T. Yamaguchi, M. Miyake, H. Watanabe, Y. Nagasawa, E. Kitagawa, S. Terada, M. Urakawa, M. T. Rose, C. D. McMahon, K. Watanabe, S. Ohwada, T. Gotoh, H. Aso
  • Journal Title: Domestic Animal Endocrinology Volume: 48 Pages: 62-68
  • DOI: 10.1016/j.domaniend.2014.01.007
  • Peer Reviewed / Open Access
• [Presentation] 経口投与したセロトニンの抗肥満作用と骨格筋エネルギー代謝に与える影響の解析 (2016)
  • Author(s): 日高湧介・寺田俊介・佐藤勝祥・海野 剛・田口輝明・陳 香凝・浦川めぐみ・渡邊康一・野地智法・麻生
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2016-03-29
• [Presentation] ウシID型筋線維の脂肪代謝能に関する免疫組織化学的解析 (2016)
  • Author(s): 對木陽淳・佐藤貴俊・野地智法・麻生 久・渡邊康一
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2016-03-28
• [Presentation] 反芻動物特異的Ⅰ型筋線維の産肉論的機能形態学解析 (2015)
  • Author(s): 渡邊康一
  • Organizer: 第65回東北畜産学会大会
  • Place of Presentation: 東北大学農学部（宮城県仙台市）
  • Year and Date: 2015-08-27
  • Invited
• [Presentation] 反芻動物特異的I型筋線維サブタイプの免疫組織化学的特性の解明 (2015)
  • Author(s): 佐藤貴俊・白須直樹・櫻田隆大・野地智法・麻生 久・渡邊康一
  • Organizer: 第119回日本畜産学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2015-03-28
• [Presentation] ウシ骨格筋への低周波電気刺激によるID型筋線維の誘導 (2014)
  • Author(s): 藤貴俊・白須直樹・櫻田隆大・野地智法・麻生 久・渡邊康一
  • Organizer: 第64回東北畜産学会大会
  • Place of Presentation: 福島市・コラッセふくしま
  • Year and Date: 2014-09-12
• [Presentation] 食肉と乳製品の食品組織学 (2014)
  • Author(s): 渡邊康一
  • Organizer: 第66回日本家政学会大会・食品組織部会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2014-05-23 – 2014-05-25
• [Presentation] ウシ最長筋における筋線維型移行に伴う筋タンパク質分解系の発現解析 (2014)
  • Author(s): 白須直樹・佐藤貴俊・櫻田隆大・野地智法・麻生 久・渡邊康一
  • Organizer: 第118回日本畜産学会大会
  • Place of Presentation: つくば市
• [Presentation] 成長および飼養管理の違いが日本短角種の筋線維型構成割合に与える影響 (2014)
  • Author(s): 小笠原英毅・岡田海渡・小林美里・小路晶子・渡邊康一・麻生 久・寳示戸雅之
  • Organizer: 第118回日本畜産学会大会
  • Place of Presentation: つくば市
• [Presentation] Immunohistochemical profiles of a ruminant-specific type I myofiber in the bovine skeletal muscles: Type ID myofiber revisited. (2013)
  • Author(s): Watanabe K., Sakurada T., Shirasu N., Sato T., Nochi T., Aso H.
  • Organizer: International Symposium on Morphological Sciences (XXIII ISMS 2013)
  • Place of Presentation: 新潟市
• [Presentation] 放牧飼養が日本短角種の枝肉成績と筋線維型構成割合に及ぼす影響 (2013)
  • Author(s): 小笠原英毅・岡田海渡・小林美里・渡邊康一・麻生 久・寳示戸雅之
  • Organizer: 第117回日本畜産学会大会
  • Place of Presentation: 新潟市
• [Book] 獣医組織学 第6版 (2014)
  • Author(s): 渡邊康一・麻生 久
  • Total Pages: 364
  • Publisher: 学窓社