The expression of prosaposin in the reproductive tissues and early embryo
| Project/Area Number |
25450466
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Ehime University |
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Principal Investigator |
Shimokawa Tetsuya 愛媛大学, 医学(系)研究科(研究院), 講師 (40363337)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 卵巣 / 卵管 / 子宮 / 初期胚 / プロサポシン / GPR37 / GPR37L1 / 神経栄養因子 / 初期発生 / in situ hybidization / Prosaposin / 受精卵 / ラット着床前胚 |
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| Outline of Final Research Achievements |
Prosaposin (PSAP) is a trophic factor and activator of sphingolipid hydrolase in lysosomes. G protein-coupled receptor (GPR)37 and GPR37L1 are receptors for prosaptide and prosaposin. Immunoblotting using oviduct and uterine tissues showed that the production of PSAP and its receptors was affected by the estrus cycle. Intense expression of PSAP mRNA, examined using in situ hybridization, was observed in rat oviducts, uterus and early embryo. In rats, alternative splicing generates two forms of mRNA coding for PSAP: Pro+9, containing a nine-base insertion, and Pro+0, which lacks the insertion. Both types of mRNA (Pro+9 and Pro+0) were detected, indicating that rat uterus contains various types of PSAP-producing and/or -secreting cells. This study revealed diverse pivotal functions for PSAP in the reproductive system.
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Report
(4 results)
Research Products
(5 results)
