Analysis of the imprinted genes regulating Cdx2 expression in mouse trophoblast stem cells.

• Project/Area Number: 25450470
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: Ogawa Hidehiko 東京農業大学, 応用生物科学部, 教授 (20339089)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胎盤形成 / 栄養膜幹細胞 / 雌雄ゲノム / 発生・分化 / 遺伝子発現制御 / 発現制御

Outline of Final Research Achievements

To clarify the function of parental genomes for the placentation in mice, the regulation of Cdx2 expression in trophoblast stem cells derived from androgenetic embryos (ATS cells) has been examined. CDX2-positive giant cells were detected in ATS cells. Moreover, Cdx2 was expressed via MEK-ERK and non-MEK-ERK pathway. These unique characteristics in ATS cells may provide the novel information to understand the function of parental genomes for Cdx2 expression.

Research Products

• [Journal Article] Cell proliferation potency is independent of FGF4 signaling in trophoblast stem cells derived from androgenetic embryos (2016)
  • Author(s): Ogawa H, Takyu R, Morimoto H, Toei S, Sakon H, Goto S, Moriya S, Kono T.
  • Journal Title: Journal of Reproduction and Development Volume: 62 Issue: 1 Pages: 51-58
  • DOI: 10.1262/jrd.2015-097
  • NAID: 130005126319
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microarray analysis of differentially expressed genes in inner cell mass and trophectoderm of parthenogenetic embryos. (2016)
  • Author(s): Goto S, Cao F, Kono T, Ogawa H.
  • Journal Title: J Mamm Ove Res Volume: 33 Pages: 45-54
  • NAID: 40020876159
  • Peer Reviewed
• [Journal Article] Deficiency of genomic reprogramming in trophoblast stem cells following nuclear transfer. (2015)
  • Author(s): Ogawa H, Watanabe H, Fukuda A, Kono T.
  • Journal Title: Cell Reprogram Volume: 17 Issue: 2 Pages: 115-23
  • DOI: 10.1089/cell.2014.0073
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epigenetic and transcriptional features of the novel human imprinted lncRNA GPR1AS suggest it is a functional ortholog to mouse Zdbf2linc. (2013)
  • Author(s): Kobayashi H, Yanagisawa E, Sakashita A, Sugawara N, Kumakura S, Ogawa H, Akutsu H, Hata K, Nakabayashi K, Kono T.
  • Journal Title: Epigenetics Volume: 8 (6) Issue: 6 Pages: 635-645
  • DOI: 10.4161/epi.24887
  • Peer Reviewed
• [Presentation] 3次元培養法を用いた栄養膜幹細胞からの胎盤様構造体構築 (2014)
  • Author(s): 小川英彦・上田真弓・河野友宏
  • Organizer: 第55回日本卵子学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2014-05-17 – 2014-05-18
• [Presentation] berrant expression of Cdx2 in trophoblast stem cells derived from androgenetic embryos. (2013)
  • Author(s): Ogawa H, Takyu R, Morimoto H, Kono T.
  • Organizer: SSR 46th Annual meeting
  • Place of Presentation: カナダ・モントリオール
• [Presentation] マウス雄核発生胚由来栄養膜幹細胞におけるCdx2の発現はFGF4に依存しない (2013)
  • Author(s): 森本洋武、武弓竜一、小川英彦、河野友宏
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学
• [Remarks] 東京農業大学応用生物科学部バイオサイエンス学科動物発生工学研究室
  • URL: http://www.nodai.ac.jp/bios/bio/laboratory/05_ade/index.html
• [Remarks] 東京農業大学応用生物科学部バイオサイエンス学科動物発生工学研究室
  • URL: http://www.nodai.ac.jp/bios/bio/laboratory/05_ade/index.html