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Thermoreversible gel disc assay of rat angiogenesis

Research Project

Project/Area Number 25450473
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionAzabu University

Principal Investigator

wakui shin  麻布大学, 獣医学部, 准教授 (40201157)

Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords血管新生 / 微小循環学 / 血管内皮細胞 / 血管周細胞 / 細胞質突起相互陥入 / 血管内皮細胞・周細胞質相互陥入 / 熱可逆性ゲルポリマー
Outline of Final Research Achievements

Endothelial cells and pericytes play critical role in angiogenesis, which is controlled, in part, by the angiopoietin (Ang)/Tie-2 system and vascular endothelial growth factor (VEGF). Endothelial cell and pericyte interdigitations(EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. After subcutaneous implantation of a thermoreversible gelation polymer disc in rats, the capillary density was low on day 5, increased to a peak on day 7, and then decreased on days 10-20. A small number of EPI were observed on day 5, then increased sharply to a peak on day 10, but had decreased on day 20.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 6 results,  Open Access: 2 results) Presentation (7 results)

  • [Int'l Joint Research] コロラド大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Male rats exposed in utero to di(n-butyl) phthalate: age-related changes in Leydig cell smooth endoplasmic reticulum and testicular testosterone-biosynthesis enzymes/ Proteins.2016

    • Author(s)
      Motohashi, M., Wempe, MF, Mutou, T., Takahashi, H., Kansaku, Ikegami, M., N.,Inomata, T., Asari, M., Wakui, S.
    • Journal Title

      Reproductive Toxicology

      Volume: 59 Pages: 139-146

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] In utero-exposed di(n-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rat.2016

    • Author(s)
      Motohashi, M., Wempe, MF, Mutou, T., Okayama, Y., Kansaku, N., Takahashi, H., Ikegami, M., Asari, M. Wakui, S.
    • Journal Title

      Journal of Toxicologic Science

      Volume: 41 Pages: 195-205

    • NAID

      130005132667

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Adenocarcinoma of the ampullary glands of the ductus deferens in a Sprague-Dawley rat.2015

    • Author(s)
      Motohashi, M., Wakui, S., Takahashi, H., Mutou, T., Yoshida, A., Inomata, T., Asari, M. and Ikegami, M.
    • Journal Title

      Toxicol. Pathol.

      Volume: 43 Pages: 593-599

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Three-dimensional reconstruction of deferent ducts papillae in urogenital duct system of the male rat.2015

    • Author(s)
      Wakui, S., Motohashi, M., Inomata, T., Mishumi, M., Mutou, T., Takahashi, H., Ikegami, M. Asari, M.
    • Journal Title

      The Prostate.

      Volume: 75 Pages: 646-652

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats2014

    • Author(s)
      Shin Wakui, Masaru Shirai, Masaya Motohashi, Tomoko Mutou, Noriko Oyama, Michael F. Wempe, Hiroyuki Takahashi, Tomoo Inomata, Masahiro Ikegami, Hitoshi Endou, and Masao Asari
    • Journal Title

      Toxicol. Pathol.

      Volume: 42 Pages: 877-887

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Adenocarcinoma of the Ampullary Glands of the Ductus Deferens in a Sprague-Dawley Rat2014

    • Author(s)
      Masaya Motohashi, Shin Wakui, Hiroyuki Takahashi, Ayaka Yoshida, Tomoko Mutou, Masahiro Ikegami, Masao Asari, and Tomo Inomata
    • Journal Title

      Toxicol. Pathol.

      Volume: 42 Pages: 00-00

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Atypical leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal Di(n-butyl) Phthlalate Exposure.2013

    • Author(s)
      39. Wakui S, Takahashi H, Shirai M, Jutabha P, Anzai N, Wempe M, Kansaku N, Hano H, Inomata T. Endou H,
    • Journal Title

      Toxicologic Pathology

      Volume: 41 Issue: 3 Pages: 480-486

    • DOI

      10.1177/0192623312457272

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Male Sprague-dawley rats exposure to in utero di(n-butyl) phthalate: dose dependent and age-related morphological change in Leydig cells smooth endoplasmic reticulum.2013

    • Author(s)
      Shirai,M., Wakui, S., Wempe, M.F., Muto, T., Oyama, N., Motohashi, M., Takahashi, H., Kansaku, N., Inomata,T., Hano, H. and Endou,H.
    • Journal Title

      Toxicol. Pathol.

      Volume: 41 Issue: 7 Pages: 984-981

    • DOI

      10.1177/0192623312474725

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Promoting effect of Di(n-butyl) phthalate on urinary bladder carcinogenesis in rats intiated by BBN.2016

    • Author(s)
      岡山祐弥、杉山光、大門孝行、本橋雅也、武藤朋子、福島昭治、和久井信
    • Organizer
      第43回日本毒性学会
    • Place of Presentation
      愛知
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
  • [Presentation] L-type amino acid transporter 1 and 4F2 heavy chain on tumor microvasculature in N-butyl-N-(4-hydroxybutyl) nitrosamine-induced rat bladder carcinoma.2016

    • Author(s)
      武藤朋子、本橋雅也、鷹橋浩幸、池上雅博、金井好克、遠藤仁、和久井信
    • Organizer
      第43回日本毒性学会
    • Place of Presentation
      愛知
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 三次元形態学的解析:SD Ratの前立腺Ampullary glands に認められたAtypical hyperplasia and Adenocarcinoma2016

    • Author(s)
      本橋雅也、吉田彩夏、鷹橋浩幸、白井勝、武藤朋子、池上雅博、和久井信
    • Organizer
      第32回日本毒性病理学会
    • Place of Presentation
      香川県
    • Year and Date
      2016-01-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Nuclear Morphometorical Analysis on Leydig Cells of Male Pubertal Rats Exposure to In Utero Di(n-butyl) phthalate2014

    • Author(s)
      本橋雅也、佐藤武美、白井勝、三隅碧、武藤朋子、鷹橋浩幸、池上雅博、遠藤仁、浅利昌男、猪股智夫、和久井信
    • Organizer
      第41回日本毒性学会
    • Place of Presentation
      神戸
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Vascular endothelial growth factor mRNA levels as a biomarker for short-term N-butyl-N-(4-hydroxybutyl) nitrosamine-induced rat bladder carcinogenesis bioassay2014

    • Author(s)
      武藤朋子、鷹橋浩幸、池上雅博、鰐淵英男、福島昭治、和久井信
    • Organizer
      第41回日本毒性学会
    • Place of Presentation
      神戸
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Wistar ラット・SD ラット腎糸球体の雌雄差に関する三次元形態学的解析2014

    • Author(s)
      木村啓成、猪股智夫、鷹橋浩幸、武藤朋子、大山紀子、羽野寛、和久井信
    • Organizer
      第30回日本毒性病理学会
    • Place of Presentation
      徳島
    • Related Report
      2013 Research-status Report
  • [Presentation] 胎生期Di(n-butyl)phthalate暴露ラットの成長に伴うLeydig細胞の超微形態学的検討2013

    • Author(s)
      白井勝、本橋昌也、鷹橋浩幸、大山紀子、武藤朋子、羽野寛、遠藤仁、浅利昌男、和久井信
    • Organizer
      第40回日本毒性学会
    • Place of Presentation
      幕張
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2022-02-16  

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