Thermoreversible gel disc assay of rat angiogenesis
Project/Area Number |
25450473
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Integrative animal science
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Research Institution | Azabu University |
Principal Investigator |
wakui shin 麻布大学, 獣医学部, 准教授 (40201157)
|
Project Period (FY) |
2013-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 血管新生 / 微小循環学 / 血管内皮細胞 / 血管周細胞 / 細胞質突起相互陥入 / 血管内皮細胞・周細胞質相互陥入 / 熱可逆性ゲルポリマー |
Outline of Final Research Achievements |
Endothelial cells and pericytes play critical role in angiogenesis, which is controlled, in part, by the angiopoietin (Ang)/Tie-2 system and vascular endothelial growth factor (VEGF). Endothelial cell and pericyte interdigitations(EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. After subcutaneous implantation of a thermoreversible gelation polymer disc in rats, the capillary density was low on day 5, increased to a peak on day 7, and then decreased on days 10-20. A small number of EPI were observed on day 5, then increased sharply to a peak on day 10, but had decreased on day 20.
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Report
(6 results)
Research Products
(16 results)
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[Journal Article] Male rats exposed in utero to di(n-butyl) phthalate: age-related changes in Leydig cell smooth endoplasmic reticulum and testicular testosterone-biosynthesis enzymes/ Proteins.2016
Author(s)
Motohashi, M., Wempe, MF, Mutou, T., Takahashi, H., Kansaku, Ikegami, M., N.,Inomata, T., Asari, M., Wakui, S.
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Journal Title
Reproductive Toxicology
Volume: 59
Pages: 139-146
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] In utero-exposed di(n-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rat.2016
Author(s)
Motohashi, M., Wempe, MF, Mutou, T., Okayama, Y., Kansaku, N., Takahashi, H., Ikegami, M., Asari, M. Wakui, S.
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Journal Title
Journal of Toxicologic Science
Volume: 41
Pages: 195-205
NAID
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Adenocarcinoma of the ampullary glands of the ductus deferens in a Sprague-Dawley rat.2015
Author(s)
Motohashi, M., Wakui, S., Takahashi, H., Mutou, T., Yoshida, A., Inomata, T., Asari, M. and Ikegami, M.
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Journal Title
Toxicol. Pathol.
Volume: 43
Pages: 593-599
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Three-dimensional reconstruction of deferent ducts papillae in urogenital duct system of the male rat.2015
Author(s)
Wakui, S., Motohashi, M., Inomata, T., Mishumi, M., Mutou, T., Takahashi, H., Ikegami, M. Asari, M.
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Journal Title
The Prostate.
Volume: 75
Pages: 646-652
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats2014
Author(s)
Shin Wakui, Masaru Shirai, Masaya Motohashi, Tomoko Mutou, Noriko Oyama, Michael F. Wempe, Hiroyuki Takahashi, Tomoo Inomata, Masahiro Ikegami, Hitoshi Endou, and Masao Asari
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Journal Title
Toxicol. Pathol.
Volume: 42
Pages: 877-887
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Male Sprague-dawley rats exposure to in utero di(n-butyl) phthalate: dose dependent and age-related morphological change in Leydig cells smooth endoplasmic reticulum.2013
Author(s)
Shirai,M., Wakui, S., Wempe, M.F., Muto, T., Oyama, N., Motohashi, M., Takahashi, H., Kansaku, N., Inomata,T., Hano, H. and Endou,H.
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Journal Title
Toxicol. Pathol.
Volume: 41
Issue: 7
Pages: 984-981
DOI
Related Report
Peer Reviewed
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