Suppression of the splicing mutation based on the molecular mechanism of pre-mRNA splicing fidelity
Project/Area Number |
25460057
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
|
Research Institution | Hokkaido University |
Principal Investigator |
MAITA Hiroshi 北海道大学, 薬学研究院, 講師 (60431318)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | スプライシング / ケミカルバイオロジー / 低分子化合物 / スプライソソーム / fidelity / スプライス部位変異 / snRNP / スプリットルシフェラーゼ |
Outline of Final Research Achievements |
We have developed an assay to find a compound that affect the spliceosome and have obtained several hit compounds by the automated screening of a small compound library. In this study we made a genetic reporter to detect the compound-mediated recovery of defected splicing that is caused by a mutation in the 3’ splice site. Some of our hit compounds have shown that they can induce splicing of the mutated intron at the correct site.
|
Report
(5 results)
Research Products
(6 results)