| Project/Area Number |
25460130
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Kitasato University |
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Principal Investigator |
Uchida Ryuji 北里大学, 薬学部, 講師 (60280632)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 抗真菌剤 / 感染症 / カイコ評価系 / 微生物資源 / 天然有機化合物 / カンジダ / 抗真菌 / カイコ感染症モデル / 天然物 / スクリーニング / カイコ / in vivo |
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| Outline of Final Research Achievements |
The in vivo-mimic assay system using silkworm larvae was performed as the primary screening tool to discover antifungal antibiotics against Candida albicans. 9,500 Microbial cultures were screened in this in vivo-mimic assay system, resulting that five known compounds showing a therapeutic effect were identified. At the same time, in the course of screening for amphotericin B potentiators of microbial origins, four new compounds, designated shodoamides A to D, were isolated from the culture broth of unidentified fungal strain BF-0158. Furthermore, stereochemistries, antifungal activities and mode of action of new antifungal antibiotics, simplifungin and valsafungins A and B, were also elucidated.
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