Search of inhibitor for the pathogenesis factor elastase. Aiming at therapeutic drug development of pulmonary aspergillosis
| Project/Area Number |
25460143
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Meijo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NIKAI Toshiaki 名城大学, 薬学部, 教授 (80103266)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | アスペルギルス / エラスターゼ / エラスターゼインヒビター / 肺アスペルギルス症 / pulmonary aspergillosis / elastase / elastase inhibitor / cytotoxicity / アスペルギルス症 |
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| Outline of Final Research Achievements |
Elastolytic proteinase produced by Aspergillus species is regarded as one of the pathogenesis factor, and hydrolyze elastin and collagen exist in lung tissues, and blood coagulation factor fibrinogen. In this study, it was intended to use elastase inhibitor derived from Aspergillus fungus as seeds of the pulmonary aspergillosis therapeutic drug development. Elastolytic proteinases isolated from culture filtrate of seven Aspergillus strains were characterized and compared their cytotoxicity on cultivated human cells. Elastase inhibitors were also purified and examined their pathogenic depression effects. As a result, it was confirmed that the inhibitor which A. fumigatus and A. flavus produced showed a strong effect for not only the elastolytic proteinases of oneself but also the elastolytic proteinases derived from other strains.
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Report
(4 results)
Research Products
(11 results)
