| Project/Area Number |
25460151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
ITO YUJI 鹿児島大学, 理工学研究域理学系, 教授 (60223195)
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| Co-Investigator(Kenkyū-buntansha) |
ARIMA Naomichi 鹿児島大学, 医歯学総合研究科, 客員研究員 (30175997)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 抗体医薬 / ガン / ファージライブラリー / 次世代シークエンサー / バイオパンイング / 単鎖Fv抗体 / ヒト抗体 / 抗体 / 治療 / 細胞 / ファージライブラリ / バイオパンニング / ガン細胞 / 網羅的配列解析 / 抗体ライブラリ |
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| Outline of Final Research Achievements |
In this study, we aimed to establish the short-term and reliable system for isolating anti-cancer antibodies (single chain Fv antibodies) from antibody phage library, especially by combining the biopanning and the comprehensive sequence analysis on next generation sequencer (NGS method). As a result, we successfully isolated several clones of lutheran and antigen A-specific antibodies which targeted hepatic and Esophageal cancers, respectively. These results indicate that NGS method is more useful in isolation of multiple antigen-specific antibodies, as compared with the conventional biopanning method comprising of biopanning and cloning/binding screening processes.
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