Development of anti-cancer antibodies through high-throughput sequencing from antibody phage library
| Project/Area Number |
25460151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
ITO YUJI 鹿児島大学, 理工学研究域理学系, 教授 (60223195)
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| Co-Investigator(Kenkyū-buntansha) |
ARIMA Naomichi 鹿児島大学, 医歯学総合研究科, 客員研究員 (30175997)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 抗体医薬 / ガン / ファージライブラリー / 次世代シークエンサー / バイオパンイング / 単鎖Fv抗体 / ヒト抗体 / 抗体 / 治療 / 細胞 / ファージライブラリ / バイオパンニング / ガン細胞 / 網羅的配列解析 / 抗体ライブラリ |
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| Outline of Final Research Achievements |
In this study, we aimed to establish the short-term and reliable system for isolating anti-cancer antibodies (single chain Fv antibodies) from antibody phage library, especially by combining the biopanning and the comprehensive sequence analysis on next generation sequencer (NGS method). As a result, we successfully isolated several clones of lutheran and antigen A-specific antibodies which targeted hepatic and Esophageal cancers, respectively. These results indicate that NGS method is more useful in isolation of multiple antigen-specific antibodies, as compared with the conventional biopanning method comprising of biopanning and cloning/binding screening processes.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Highly efficient production of VHH antibody fragments in Brevibacillus choshinensis expression system2015
Author(s)
Makoto Mizukami, Hiroko Tokunaga, Hiromasa Onishi, Yohei Ueno, Hiroshi Hanagata, Nobuo Miyazaki, Norihiko Kiyose, Yuji Ito, Matsujiro Ishibashi, Yoshihisa Hagihara, Tsutomu Arakawa, Akira Miyauchi, Masao Tokunaga
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Journal Title
Protein Expression and Purification
Volume: 105
Pages: 23-32
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Patent(Industrial Property Rights)] 抗癌剤2015
Inventor(s)
吉川大和, 野水基義, 福原武志 , 伊東祐二
Industrial Property Rights Holder
吉川大和, 野水基義, 福原武志 , 伊東祐二
Industrial Property Rights Type
特許
Industrial Property Number
2015-230926
Filing Date
2015-11-26
Related Report
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