| Project/Area Number |
25460199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKURA TAKASHI 帝京大学, 薬学部, 准教授 (80326123)
HIGUCHI KEI 帝京大学, 薬学部, 助教 (10625304)
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| Co-Investigator(Renkei-kenkyūsha) |
HOSOYAMADA MAKOTO 帝京大学, 薬学部, 教授 (00291659)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血液脳関門 / ヒト脳移行性 / トランスポーター / 有機カチオン性薬物 / ヒト血液脳関門モデル細胞 / 循環培養システム / フロー培養システム / 血液脳関門モデル細胞 |
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| Outline of Final Research Achievements |
The objectives of this study are to develop reliable experimental-method(s) for predicting functions of human blood-brain barrier (BBB), using hCMEC/D3 cells, an in vitro human BBB model. As for the research achievements, 1) the in vitro cocktail dosing method could be developed for evaluating the human BBB function. In addition, in vivo microdialysis method with cocktail-dosing could be developed. The latter method will be important for making the preclinical study more efficient. 2) We could gain the important information about a novel and flow-based cultivation method with shear stress.
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