| Project/Area Number |
25460241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kumamoto University (2015-2016)
Kanazawa University (2013-2014) |
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Principal Investigator |
Wakayama Tomohiko 熊本大学, 大学院生命科学研究部(医), 教授 (70305100)
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| Co-Investigator(Renkei-kenkyūsha) |
KATO Yukio 金沢大学, 医薬保健研究域薬学系, 教授 (30251440)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 精巣 / 精子形成 / 細胞接着分子 / 不妊症 / 精子完成 / セルトリ細胞 / アダプター蛋白質 / ノックアウトマウス / 造精細胞 / 免疫沈降 / 抗体作製 |
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| Outline of Final Research Achievements |
The cell adhesion molecule-1 (CADM1) is expressed in only spermatogenic cells but not Sertoli or Leydig cells. CADM1 expressed in spermatogenic cells interacts with Poliovirus receptor on Sertoli cells. CADM1-deficient mice represent male infertility due to defective spermatogenesis, in which spermatids are detached and the remaining spermatids show abnormal morphology. We could identify B-box and SPRY-domain containing protein (BSPRY) as an adaptor protein of CADM1 by yeast-two hybrid method. To clarify the expression and cellular localization of BSPRY in testis of wild type and CADM1-deficient mice. Immunohistochemistry demonstrated that BSPRY is localized in the step 11 to 16 spermatids. The levels of BSPRY in CADM1-deficient mice decreased by 70% compared with wild type ones by western blot analysis. These results suggested that the deficiency of CADM1 caused that the expression of its adaptor protein BSPRY decreased in elongating spermatids.
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