Histological and molecular analysis of developmental process of the midline facial skeleton

• Project/Area Number: 25460254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tokyo University of Science
• Principal Investigator: Wada Naoyuki 東京理科大学, 理工学部, 教授 (50267449)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 顎顔面発生 / 頭蓋底軟骨 / 鼻中隔 / 神経堤細胞 / 発生系譜 / 糖鎖 / 頭顔面発生 / 軟骨分化 / レクチン

Outline of Final Research Achievements

The developmental process and origin of the anterior part of skull base cartilage has not been unclear. In this project, we analyzed the origin of the cartilage by focusing on the expression of a novel carbohydrate chain, PNA-BM (peanut-agglutinin lectin binding molecule).
Two molecules were detected by PNA-lectin blotting analysis. Fate mapping analysis and tissue removal experiment of cranial neural crest cells showed that expression of PNA-BM correlated to the origin of the cartilage. The expression was weak in the cells of paired rod-like cartilage, the trabecular cartilage (TC), whereas strong expression of it was observed in the cells of inter-trabecular cartilage (ITC), which connects the anterior end of TC. In TC cells, actin polymerization was unclear, while in PNA-BM positive ITC cells, the polymerization was apparent. These results suggest that cells in TC and ITC have distinct properties, although both are histologically designated as chondrocytes.

Research Products

• [Journal Article] Short-term feeding at the wrong time is sufficient to desynchronize peripheral clocks and induce obesity with hyperphagia, physical inactivity and metabolic disorders in mice (2016)
  • Author(s): Yasumoto Y, Hashimoto C, Nakao R, Yamazaki H, Hiroyama H, Nemoto T, Yamamoto S, Sakurai M, Oike H, Wada N, Yoshida-Noro C, Oishi K
  • Journal Title: Metabolism Volume: 65 Issue: 5 Pages: 714-727
  • DOI: 10.1016/j.metabol.2016.02.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mon1-Ccz1 activates Rab7 only on late endosome and dissociates from lysosome in mammalian cells (2016)
  • Author(s): Sayaka Yasuda, So Morishita, Akane Fujita, Tomohisa Nanao, Naoyuki Wada, Satoshi Waguri, Giampietro Schiavo, Mitsunori Fukuda, and Takeshi Nakamura
  • Journal Title: J. Cell Sci. Volume: 129 Pages: 329-340
  • DOI: 10.1242/jcs.178095
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distinct populations within Isl1 lineages contribute to appendicular and facial skeletogenesis through the β-catenin pathway (2014)
  • Author(s): Akiyama, R., Kawakami, H., Taketo, M. M., Evans, S. M., Wada, N., Petryk. A., Kawakami, Y.
  • Journal Title: Developmental Biology Volume: 387 Issue: 1 Pages: 37-48
  • DOI: 10.1016/j.ydbio.2014.01.001
  • Peer Reviewed
• [Journal Article] GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. (2013)
  • Author(s): 2. Fujita, A., Koinuma, S., Yasuda, S., Nagai, H., Kamiguchi, H., Wada, N., and Nakamura, T.
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: 9593-9601
  • DOI: 10.1371/journal.pone.0079689
  • Peer Reviewed
• [Journal Article] Longest neurite-specific activation of Rap1B in hippocampal neurons contributes to polarity formation through RalA and Nore1A in addition to PI3-kinase. (2013)
  • Author(s): Nakamura T, Yasuda S, Nagai H, Koinuma S, Morishita S, Goto A, Kinashi T, Wada N.
  • Journal Title: Genes Cells. Volume: 18(11) Issue: 11 Pages: 1020-31
  • DOI: 10.1111/gtc.12097
  • Peer Reviewed
• [Presentation] マウス頭蓋底軟骨原基の初期形成過程の観察 (2016)
  • Author(s): 高橋昂平，和田直之
  • Organizer: 筑波大学・東京理科大学生命医科学研究所，合同リトリート
  • Place of Presentation: オークラフロンティアホテル（つくば）
  • Year and Date: 2016-03-19
• [Presentation] ゼブラフィッシュヒレ再生の方向性 (2015)
  • Author(s): 和田直之，植本俊明
  • Organizer: 日本動物学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] レクチン染色によるゼブラフィッシュ初期軟骨組織の検出 (2015)
  • Author(s): 大西雅子，和田直之
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内キャンパス（仙台市）
  • Year and Date: 2015-09-13
• [Presentation] ニワトリ胚頭顔面形成時に内側鼻隆起で発現する遺伝子の解析 (2015)
  • Author(s): 渡邊知明，和田直之
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内キャンパス（仙台市）
  • Year and Date: 2015-09-13
• [Presentation] 梁軟骨とそれに挟まれた軟骨の不連続性の検討 (2015)
  • Author(s): 小林俊祐，和田直之
  • Organizer: Tokyo Vertebrate Morphology Meeting
  • Place of Presentation: 東京慈恵会医科大学（東京）
  • Year and Date: 2015-08-11
• [Presentation] ニワトリ胚頭部軟骨発生過程で部位特異的に発現するPNAレクチン結合分子
  • Author(s): 佐々木孝太，飯田小百合，齊藤嘉之，和田直之
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] ニワトリ胚の頭蓋底正中部で発現するPNA結合分子の発現変動
  • Author(s): 和田直之，佐々木孝太，齊藤嘉之，飯田小百合
  • Organizer: 日本解剖学会
  • Place of Presentation: 自治医科大学