Analysis of the transcription factor crosstalk in high endothelial venule formation

• Project/Area Number: 25460268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Kinki University (2015); Osaka University (2013-2014)
• Principal Investigator: HAYASAKA Haruko 近畿大学, 理工学部, 准教授 (70379246)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管内皮細胞 / 転写因子 / リンパ節 / 分化 / 内皮細胞分化

Outline of Final Research Achievements

The high endothelial venules (HEVs) are specialized blood vessels that permit lymphocyte trafficking across their endothelial cells (ECs) and develop in a tissue-specific manner. We previously found that a transcription factor X is preferentially expressed in immature HEV-ECs of lymph nodes and Peyer's patches. We found that neonatal HEVs in the gene X -knockout (KO) mice express lower levels of HEV-EC marker proteins than wild type littermates. In contrast, postnatal HEVs in the gene X-transgenic mice showed an increase in HEV-EC marker proteins. These results support the hypothesis that the gene X contributes to HEV-EC differentiation.

Research Products

• [Int'l Joint Research] Pecs University(Hungary)
• [Journal Article] Fibroblastic reticular cell-derived lysophosphatidic acid regulates confined intranodal T-cell motility. (2016)
  • Author(s): Takeda A, Kobayashi D, Aoi K, Sasaki N, Sugiura Y, Igarashi H, Tohya K, Inoue A, Hata E, Akahoshi N, Hayasaka H, Kikuta J, Scandella E, Ludewig B, Ishii S, Aoki J, Suematsu M, Ishii M, Takeda K, Jalkanen S, Miyasaka M, Umemoto E.
  • Journal Title: Elife Volume: 5
  • DOI: 10.7554/elife.10561
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lysophosphatidic acid receptors LPA4 and LPA6 differentially promote lymphocyte transmigration across high endothelial venules in lymph nodes. (2016)
  • Author(s): Hata E, Sasaki N, Takeda A, Tohya K, Umemoto E, Akahoshi N, Ishii S, Bando K, Abe T, Kano K, Aoki J, Hayasaka H, Miyasaka M
  • Journal Title: Int Immunol. Volume: なし Issue: 6 Pages: 283-292
  • DOI: 10.1093/intimm/dxv072
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Divergence of vascular specification in visceral lymphoid organs - genetic determinants and differentiation checkpoints. (2016)
  • Author(s): Kellermayer Z, Hayasaka H, Kajtar B, Simon D, Robles EF, Martinez-Climent JA, Balogh P.
  • Journal Title: Int Rev Immunol. Volume: In press Issue: 6 Pages: 489-502
  • DOI: 10.3109/08830185.2015.1059427
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The HIV-1 gp120/CXCR4 axis promotes CCR7 ligand-dependent CD4 T cell migration: CCR7 homo- and CCR7/CXCR4 hetero-oligomer formation as a possible mechanism for up-regulation of functional CCR7. (2015)
  • Author(s): Hayasaka H, Kobayashi D, Yoshimura H, Nakayama EE, Shioda T, Miyasaka M.
  • Journal Title: PLoS ONE. Volume: 10 Issue: 2 Pages: 1371-1371
  • DOI: 10.1371/journal.pone.0117454
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic changes in endothelial cell adhesion molecule nepmucin/CD300LG expression under physiological and pathological conditions. (2013)
  • Author(s): Umemoto, E, Takeda, A., Jin, S., Luo, Z., Nakahogi, N., Hayasaka, H., Lee, C.M., Tanaka, T. & Miyasaka, M.
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: 1-12
  • DOI: 10.1371/journal.pone.0083681
  • Peer Reviewed
• [Journal Article] ケモカイン受容体の協働作用による効率的なリンパ球移動の誘導 (2013)
  • Author(s): 小林 大地, 宮坂 昌之, 早坂 晴子
  • Journal Title: 生体の科学 Volume: 64 Pages: 398-399
• [Presentation] CCR7 ホモ多量体形成による細胞遊走調節機構 (2015)
  • Author(s): 小林大地、宮坂昌之、早坂晴子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場 (兵庫県）
  • Year and Date: 2015-12-02
• [Presentation] The role of the transcription factor Dach1 in the high endothelial venule development (2015)
  • Author(s): Haruko Hayasaka, Peter Balogh, Masayuki Miyasaka,
  • Organizer: 日本免疫学会
  • Place of Presentation: 札幌コンベンションセンター (北海道）
  • Year and Date: 2015-11-19
• [Presentation] 癌リンパ節転移研究における毛細リンパ管モデルの利用 (2014)
  • Author(s): 早坂 晴子 宮坂 昌之
  • Organizer: 三次元生体組織構築シンポジウム
  • Place of Presentation: 大阪大学中之島センター
  • Year and Date: 2014-12-09
  • Invited
• [Presentation] Chemokine receptor oligomerization: a potential mechanism for regulating lymphocyte and cancer cell migration
  • Author(s): Haruko Hayasaka
  • Organizer: 日本生物物理学会
  • Place of Presentation: Kyoto
  • Invited
• [Presentation] CXCR4 ligands promote CCR7-dependent CD4 T cell migration - possible involvement of CCR7 oligomerization
  • Author(s): Haruko Hayasaka, Daichi Kobayashi, Masayuki Miyasaka
  • Organizer: 日本免疫学会
  • Place of Presentation: Chiba