Development of whole heart calcium imaging system with two different waves using modified G-CaMP-DsRed-coexpressed transgenic rat model in the blood-perfused excised heart preparation
| Project/Area Number |
25460283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Gifu University |
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Principal Investigator |
Obata Koji 岐阜大学, 医学(系)研究科(研究院), 助教 (40378229)
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| Co-Investigator(Kenkyū-buntansha) |
Takaki Miyako 奈良県立医科大学, 医学部, 研究員 (00033358)
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| Co-Investigator(Renkei-kenkyūsha) |
Nakai Junichi 埼玉大学, 理工学研究科, 教授 (80237198)
Ohkura Masamichi 埼玉大学, 理工学研究科, 教授 (70369172)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | カルシウム / カルシウムイメージング / 心臓 / エネルギー消費 / 酸素消費 / 収縮性 / 心筋細胞 / 興奮収縮連関 / カルシウムイオン / イメージング / 筋収縮 / GECIs / エネルギー代謝 / G-CaMP / 総機械的エネルギー / カルシウムトランジェント / 遺伝子組換え |
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| Outline of Final Research Achievements |
I attempted to develop a whole heart calcium imaging system with two different waves using modified G-CaMP-DsRed-coexpressed transgenic rat model to investigate the relationship among cardiac contractility, oxygen consumption, and intracellular calcium handling in the blood-perfused excised heart preparation. I just have to overcome a couple of problems for measuring accurate calcium transient. Now, I try to find the effective breakthrough.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Hypergravity provokes a temporary reduction in CD4+CD8+ thymocyte number and a persistent decrease in medullary thymic epithelial cell frequency in mice.2015
Author(s)
Tateishi R, Akiyama N, Miyauchi M, Yoshinaga R, Sasanuma H, Kudo T, Shimbo M, Shinohara M, Obata K, Inoue J, Shirakawa M, Shiba D, Asahara H, Yoshida N, Takahashi S, Morita H, Akiyama T
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Journal Title
PLOS ONE
Volume: 10
Issue: 10
Pages: 1-17
DOI
Related Report
Peer Reviewed / Open Access
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