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Development of whole heart calcium imaging system with two different waves using modified G-CaMP-DsRed-coexpressed transgenic rat model in the blood-perfused excised heart preparation

Research Project

Project/Area Number 25460283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionGifu University

Principal Investigator

Obata Koji  岐阜大学, 医学(系)研究科(研究院), 助教 (40378229)

Co-Investigator(Kenkyū-buntansha) Takaki Miyako  奈良県立医科大学, 医学部, 研究員 (00033358)
Co-Investigator(Renkei-kenkyūsha) Nakai Junichi  埼玉大学, 理工学研究科, 教授 (80237198)
Ohkura Masamichi  埼玉大学, 理工学研究科, 教授 (70369172)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsカルシウム / カルシウムイメージング / 心臓 / エネルギー消費 / 酸素消費 / 収縮性 / 心筋細胞 / 興奮収縮連関 / カルシウムイオン / イメージング / 筋収縮 / GECIs / エネルギー代謝 / G-CaMP / 総機械的エネルギー / カルシウムトランジェント / 遺伝子組換え
Outline of Final Research Achievements

I attempted to develop a whole heart calcium imaging system with two different waves using modified G-CaMP-DsRed-coexpressed transgenic rat model to investigate the relationship among cardiac contractility, oxygen consumption, and intracellular calcium handling in the blood-perfused excised heart preparation. I just have to overcome a couple of problems for measuring accurate calcium transient. Now, I try to find the effective breakthrough.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results)

  • [Journal Article] Hypergravity provokes a temporary reduction in CD4+CD8+ thymocyte number and a persistent decrease in medullary thymic epithelial cell frequency in mice.2015

    • Author(s)
      Tateishi R, Akiyama N, Miyauchi M, Yoshinaga R, Sasanuma H, Kudo T, Shimbo M, Shinohara M, Obata K, Inoue J, Shirakawa M, Shiba D, Asahara H, Yoshida N, Takahashi S, Morita H, Akiyama T
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 10 Pages: 1-17

    • DOI

      10.1371/journal.pone.0141650

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility of a short-arm centrifuge for mouse hypergravity experiments2015

    • Author(s)
      Morita H, et al.
    • Journal Title

      PLOS ONE

      Volume: 10(7): e133981 Issue: 7 Pages: 1-14

    • DOI

      10.1371/journal.pone.0133981

    • NAID

      120007136296

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Possible peripheral mechanism for taste disorder in S-1 administered rats.2014

    • Author(s)
      Aoki K, Obata K, Kurihara M, Kuniyasu H, Kirita T, Takaki M
    • Journal Title

      Int J Clin Oncol

      Volume: 19 Issue: 3 Pages: 549-556

    • DOI

      10.1007/s10147-013-0572-3

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Persistent overexpression of SERCA2a affects bladder functionsunder physiological conditions, but not in bladder outlet obstruction-induced sub-acute pathological conditions.2014

    • Author(s)
      Yamada A, Torimoto K, Obata K, Hirayama A, Fujimoto K, Takaki M
    • Journal Title

      J Physiol Sci

      Volume: 64 Issue: 1 Pages: 21-30

    • DOI

      10.1007/s12576-013-0286-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vivo imaging of enteric neurogenesis in the deep tissue of mouse smallintestine. hearts of SERCA2a transgenic rats.2013

    • Author(s)
      Goto K, Obata K, Takaki M, 他
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 1 Pages: e54814-e54814

    • DOI

      10.1371/journal.pone.0054814

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Left ventricular mechanical and energetic changes in long-term isoproterenol-induced hypertrophied.2013

    • Author(s)
      Mitsuyama S, Obata K, Takaki M, 他2名
    • Journal Title

      J Mol Cell Cardiol.

      Volume: 59 Pages: 95-106

    • DOI

      10.1016/j.yjmcc.2013.02.012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new calpain inhibitor protects left ventricular dysfunction induced by mild ischemia-reperfusion in in situ rat hearts.2013

    • Author(s)
      Takeshita D, Obata K, Takak M, 他6名
    • Journal Title

      JPhysiol Sci

      Volume: 63 Issue: 2 Pages: 113-123

    • DOI

      10.1007/s12576-012-0243-6

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 左心室力学的エネルギー学的性質への新規ミオシンアクチベーター, omecamtiv mecarbilの効果2016

    • Author(s)
      小畑孝二、森田啓之、高木 都
    • Organizer
      第93回日本生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット摘出心臓の血液交叉灌流実験系を用いたメカノエナジェティクスとカルシウムイメージング解析法の開発2015

    • Author(s)
      小畑孝二、森田啓之、高木 都
    • Organizer
      第62回中部日本生理学会
    • Place of Presentation
      富山
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 左心室力学的エネルギー学的性質へのミオシンアクチベーターとSERCAアクチベーターの効果の検討2015

    • Author(s)
      小畑孝二、森田啓之、高木 都
    • Organizer
      第25回日本病態生理学会
    • Place of Presentation
      松山
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット摘出心臓における高温条件下での陰性変力作用について ― TRPV1の関与 ―2015

    • Author(s)
      小畑孝二、森田 啓之、高木 都
    • Organizer
      第120回日本解剖学会総会全国学術集会・第92回日本生理学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] ラット摘出心臓の血液交叉灌流実験系を用いた高温条件による陰性変力作用のメカニズムについて2014

    • Author(s)
      小畑孝二、安部 力、森田 啓之、高木 都
    • Organizer
      第24回日本病態生理学会
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2014-08-09 – 2014-08-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 病的心臓のメカノエナジェティクスにおけるSERCA2a過剰発現の影響2014

    • Author(s)
      小畑孝二、光山 晋一、竹下 大輔、森田 啓之、高木 都
    • Organizer
      第35回日本循環制御医学会
    • Place of Presentation
      九州大学医学部 百年講堂
    • Year and Date
      2014-07-04 – 2014-07-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 液交叉灌流実験系を用いたラット摘出心臓のカプサイシン投与によるメカノエナジェティクス解析―高温との比較―2014

    • Author(s)
      小畑孝二、その他
    • Organizer
      第91回日本生理学会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] SERCA2a過剰発現ラットにおけるイソプロテレノール誘導肥大心の左心室メカノエナジェティクスに関する研究2013

    • Author(s)
      小畑孝二、その他
    • Organizer
      第23回日本病態生理学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 血液交叉灌流実験系を用いたラット摘出心臓の高温条件下におけるメカノエナジェティクス解析2013

    • Author(s)
      小畑孝二、その他
    • Organizer
      第60回中部日本生理学会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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