• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A study on signaling pathways associated with NOX4/NADPH oxidase for the development of new antifibrotic drugs

Research Project

Project/Area Number 25460339
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General pharmacology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Katsuyama Masato  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60315934)

Co-Investigator(Renkei-kenkyūsha) ARAKAWA Noriaki  横浜市立大学, 生命ナノシステム科学研究科, 助教 (60398394)
YABE Chihiro (NISHIMURA Chihiro)  京都府立医科大学, 大学院医学研究科, 教授 (70150571)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords活性酸素 / NADPHオキシダーゼ / 線維化
Outline of Final Research Achievements

It is well known that NOX4/NADPH oxidase is involved in fibrogenesis. Signaling pathways upstream and downstream of NOX4 were analyzed. A Smad binding element at approx. 75-base upstream of the transcription start site of the NOX4 gene was found essential for TGF-β-induced NOX4 expression. Proteomics analyses using cleavable isotope-coded affinity tags were carried out on a human lung fibroblast cell line stimulated with TGF-β to identify proteins that oxidized thiols were decreased by knock-down of NOX4. Among them, Protein X, an enzyme that has been thought to be involved in polymerization of extracellular matrices, was found decreased by knock-down of NOX4 at mRNA levels. On the other hand, Protein Z, of which function is unknown, was suggested to be a target of NOX4-derived reactive oxygen species (ROS). In a human neuroblastoma cells in which NOX4 is involved in cell proliferation, Protein A, a growth factor receptor, was suggested to be a target of NOX4-derived ROS.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015 2014

All Presentation (3 results) (of which Invited: 1 results)

  • [Presentation] ほ乳類におけるNOX/NADPH oxidaseの生理機能2015

    • Author(s)
      勝山真人、矢部千尋
    • Organizer
      BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] NOX4/NADPHオキシダーゼ由来活性酸素種の標的蛋白の探索2015

    • Author(s)
      勝山真人、荒川憲昭、矢部千尋
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] NOX/NADPHオキシダーゼの多彩な機能と病態への関与2014

    • Author(s)
      勝山真人
    • Organizer
      第31回臨床フリーラジカル会議
    • Place of Presentation
      亀岡
    • Year and Date
      2014-12-05
    • Related Report
      2014 Research-status Report
    • Invited

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi