Study of lung cancer cell invasion mechanism through PtdIns imaging

• Project/Area Number: 25460433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Akashi Takumi 東京医科歯科大学, 医学部附属病院, 准教授 (60242202)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肺癌 / 浸潤 / アクチン / ホスファチジルイノシトール / 基底膜 / ミオシン / ラミニン / phosphatidylinositol / 皮質アクチン線維 / phosphatidylinositol4,5P / 浸潤突起 / フォスファチジルイノシトール

Outline of Final Research Achievements

Alterations in subcellular localization of cortical actin in lung adenocarcinomas and its correlation with metastasis and poor prognosis was clarified by histological examination of surgically resected tissues. In 3D-cultured lung cancer A549 cell spheroids, accumulation of PtdIns(4,5)P2 monitored by GFP-tagged PH-PLCD probe and phosphorylated myosin light chain as well as F-actin were observed. The basement membrane was considered to be one of the regulator of cortical actin accumulation through phosphorylated myosin-mediated contraction and PtdIns(4,5)P2-mediated F-actin assembly. Myosin inhibitor Blebbistatin suppressed 3D collective migration of A549 cells induced by constitutively active Cdc42 and MT1-MMP which suggests the participation of matrix-side F-actin in invasion through myosin-mediated contraction.

Research Products

• [Journal Article] 肺腺癌における皮質アクチン線維の細胞内局在と浸潤との相関 (2015)
  • Author(s): 冨井 翔平, 明石 巧, 安藤 登, 鈴木 好美, 古川 明日香, 江石 義信
  • Journal Title: 日本病理学会会誌 Volume: 104 Pages: 453-453
• [Journal Article] Localization of cortical actin filaments and its correlation with invasion and metastasis in lung adenocarcinomas (2015)
  • Author(s): Shohei Tomii、Takumi Akashi, Noboru Ando, Sekine Masaki, Yoshimi Suzuki, Asuka Furukawa, Yoshinobu Eishi
  • Journal Title: Proceedings of The L.Japanese Society of Pathology Volume: 104 Pages: 453-453
• [Journal Article] Propionibacterium acnes infection in glandular epithelium and stromal macrophages of the prostate with or without cancer (2014)
  • Author(s): Bae Y, Ito T, Iida T, Uchida K, Sekine M, Nakajima Y, Kumagai J, Yokoyama T, Kawachi H, Akashi T, Eishi Y
  • Journal Title: PloS one Volume: 9
  • Peer Reviewed / Open Access
• [Journal Article] The expression profiles of acidic epithelial keratins in Ameloblastoma. (2013)
  • Author(s): Pal KS, Sakamoto K, Aragaki T, Akashi T, Yamaguchi A
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Volume: 115 Issue: 4 Pages: 523-531
  • DOI: 10.1016/j.oooo.2013.01.017
  • Peer Reviewed
• [Journal Article] Higher serum CCL17 may be a promising predictor of acute exacerbations in chronic hypersensitivity pneumonitis (2013)
  • Author(s): Miyazaki Y, Unoura K, Tateishi T, Akashi T, Takemura T, Tomita M, Inase N, Yoshizawa Y:
  • Journal Title: Respiratory Res Volume: 14 Pages: 57-57
  • Peer Reviewed