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Semi-comorehensive analysis of gene amplification in solid carcinomas using MLPA and FISH

Research Project

Project/Area Number 25460452
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionKanazawa University

Principal Investigator

Ooi Akishi  金沢大学, 医学系, 教授 (50160411)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords遺伝子増幅 / MLPA / FISH / 分子標的療法 / 胃癌 / 乳癌 / 大腸癌 / 脂肪肉腫 / 受容体チロシンキナーゼ / gene amplification / gastric cancer / ERBB2 / EGFR / FGFR2 / MYC
Outline of Final Research Achievements

Gene amplification is a major type of genetic aberration found in human cancers of solid types. The amplified genes so far have been useful targets or will be potential targets for molecular therapies. In this study, cancers of the stomach, breast and colon, which are frequent among Japanese, were examined for amplification of 46 genes by multiplex ligatioin-dependent probe amplification followed by fluoresence in situ hybridization. The results showed that the genes coding receptor tyrosine kinases (ERBB2, FGFR1, FGFR2, EGFR, MET), regulatory genes of cell cycle progression (CCNE1, CCND1, CDK6, MDM2), and gene coding a transcription factor (MYC) were found frequently amplified in these tumors.
In conclusion, combination of MLPA and FISH analysis is a feasible approach for obtaining the semi-comprehensive genetic information that is necessary for personalized molecular targeted therapy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (31 results)

All 2015 2014 2013

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Acknowledgement Compliant: 5 results,  Open Access: 4 results) Presentation (20 results)

  • [Journal Article] Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.2015

    • Author(s)
      Ooi A, Oyama T, Nakamura R, Tajiri R, Ikeda H, Fushida Y, Nakamura H, Dobashi Y.
    • Journal Title

      Modern Pathology

      Volume: in press Issue: 6 Pages: 861-871

    • DOI

      10.1038/modpathol.2015.33

    • NAID

      120005593968

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and regulatory effects on cancer cell behavior of NELL1 and NELL2 in human renal cell carcinoma2015

    • Author(s)
      Nakamura R, Oyama T, Tajiri R, Mizokami A, Namiki, M, Nakamoto M, Ooi A
    • Journal Title

      Cancer Sci

      Volume: 106 Issue: 5 Pages: 656-664

    • DOI

      10.1111/cas.12649

    • NAID

      120005651443

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Diverse involvement of isoforms and gene aberrations of Akt in human lung carcinomas2015

    • Author(s)
      Dobashi Y, Tsubochi H, Matsubara H, Inoue J, Inazawa J, Endo S, Ooi A
    • Journal Title

      Cancer Sci

      Volume: - Issue: 6 Pages: 772-781

    • DOI

      10.1111/cas.12669

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Overexpression and gene amplification of both ERBB2 and EGFR in an esophageal squamous cell carcinoma revealed by fluorescence in situ hybridization, multiplex ligation-dependent probe amplification and immunohistochemistry.2015

    • Author(s)
      Takeru Oyama, Koichi Okamoto、 Ritsuko Nakamura, Ryosuke Tajiri, Hiroko Ikeda, Itasu Ninomiya , Akishi Ooi
    • Journal Title

      Pathol Int

      Volume: 65 Issue: 11 Pages: 608-613

    • DOI

      10.1111/pin.12344

    • NAID

      120005661920

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Co-active receptor tyrosine kinases mitigate the effect of FGFR inhibitors in FGFR1-amplified lung cancers with low FGFR1 protein expression2015

    • Author(s)
      Hiromichi Ebi, Hiroshi Kotani, Hidenori Kitai, Shigeki Nanjo, Kenji Kita, Seiji Yano, Akishi Ooi, Tiffany Huynh, Mari Mino-Kenudson, and Anthony C. Faber
    • Journal Title

      Oncogene

      Volume: 未定 Issue: 27 Pages: 3587-97

    • DOI

      10.1038/onc.2015.426

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Intratumoral heterogeneous amplification of ERBB2 and subclonal genetic diversity in gastric cancers revealed by multiple ligation-dependent probe amplification and fluorescence in situ hybridization.2014

    • Author(s)
      Tajiri. R., Ooi. A., Fujimura, T., Dobashi, Y., Oyama, T., Nakamura, R. and Ikeda, H.
    • Journal Title

      Hum Pathol.

      Volume: 45(4) Issue: 4 Pages: 725-34

    • DOI

      10.1016/j.humpath.2013.11.004

    • NAID

      120005440337

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic aberrations as the targets of oncology research: Involvement of paraffin-embedded tissues2014

    • Author(s)
      Dobashi Y, Goto A, Endo T, Ooi A
    • Journal Title

      Histol Histopathol

      Volume: 29 Pages: 191-205

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comparative immunohistochemical analysis of IMP3, GLUT1, EMA, CD146, and desmin for distinguishing malignant mesothelioma from reactive mesothelial cells.2014

    • Author(s)
      Minato H, Sagawa M, Sakuma T, et al.
    • Journal Title

      Am J Clin Pathol

      Volume: 141 Issue: 1 Pages: 85-93

    • DOI

      10.1309/ajcp5knl7qtellyi

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significance of Akt activation and AKT gene increases in soft tissue tumors.2014

    • Author(s)
      Dobashi Y, Inazawa J, et al.
    • Journal Title

      Hum Pathol.

      Volume: 45 Issue: 1 Pages: 127-36

    • DOI

      10.1016/j.humpath.2013.06.024

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clonal profiling of mixed lobular and ductal carcinoma revealed by multiplex ligation-dependent probe amplification and fluorescence in situ hybridization2014

    • Author(s)
      Tajiri R, Inokuchi M, Sawada-Kitamura S, Kawashima H, Nakamura R, Oyama T, Dobashi Y, Ooi A
    • Journal Title

      Pathol Int

      Volume: 64 Issue: 5 Pages: 231-236

    • DOI

      10.1111/pin.12158

    • NAID

      120005463366

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Utility of fluorescence in situ hybridization to detect MDM2 amplification in liposarcomas and their morphological mimics.2013

    • Author(s)
      Hiroaki Kimura, Yoh Dobashi, Takayuki Nojima, Hiroyuki Nakamura, Norio Yamamoto, Hiroyuki Tsuchiya, Hiroko Ikeda, Seiko Sawada-Kitamura, Takeru Oyama, Akishi Ooi
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 6 Pages: 1306-1316

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 1.MLPAとFISHを用いた、進行胃癌の遺伝子増幅の準網羅的検索2015

    • Author(s)
      大井章史、尾山武、中村律子、田尻亮輔。池田博子、土橋洋
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 2.アレイCGH法を用いたヒト胃癌に対するゲノムコピー数異常の網羅的解析2015

    • Author(s)
      尾山武、中村律子、田尻亮輔、大井章史
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 3.膀胱尿路上皮癌におけるFISH法を用いたCCND1およびMDM2遺伝子増幅の検索2015

    • Author(s)
      田尻亮輔、中村律子、尾山武、大井章史
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] MLPAとFISHを用いた胃癌における増幅遺伝子の準網羅的検索2014

    • Author(s)
      大井 章史、田尻 亮輔、尾山 武、中村 律子、伏田 幸夫、土橋 洋
    • Organizer
      73th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      PACIFICO YOKOHAMA, Yokohama
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 肺癌におけるAKT遺伝子増加、isoformの発現異常と病的意義2014

    • Author(s)
      土橋 洋、坪地 宏嘉、井上 純、大井 章史
    • Organizer
      73th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      PACIFICO YOKOHAMA, Yokohama
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] FISH法を用いた膀胱尿路上皮癌におけるCCND1遺伝子増幅の検索2014

    • Author(s)
      田尻 亮輔、尾山 武、中村 律子、大井 章史
    • Organizer
      73th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      PACIFICO YOKOHAMA, Yokohama
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] アレイCGH法による癌関連遺伝子発現の腫瘍内ヘテロ不均一性を示す部分間におけるゲノムコピー数異常解析2014

    • Author(s)
      尾山 武、中村 律子、田尻 亮輔、大井 章史
    • Organizer
      73th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      PACIFICO YOKOHAMA, Yokohama
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Multiple ligation-dependent probe amplificationとFISHを用いた胃癌における遺伝子増幅の準網羅的検索2014

    • Author(s)
      大井章史、田尻亮輔、中村律子、尾山武、池田博子、土橋洋
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト肺癌におけるAKT遺伝子、蛋白質の異常とその意義2014

    • Author(s)
      土橋洋、椙村春彦、山田茂樹、大井章史
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 腎癌における分泌型タンパクNell1/2の発現機能解析2014

    • Author(s)
      中村律子、尾山武、田尻良輔、大井章史
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト胃癌における癌関連遺伝子発現の腫瘍内不均一性を示す部分間における遺伝子発現の相違2014

    • Author(s)
      尾山武、中村律子、田尻良輔、大井章史
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場、広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Multiplex-ligation dependent probe amplification を用いた 胃癌における分子標的の準網羅的検索。Research Forum 7. 胃腫瘍診療の最前線2014

    • Author(s)
      大井章史、藤村隆
    • Organizer
      第100回日本消化器病学会総会
    • Place of Presentation
      東京国際フォーラム、東京
    • Year and Date
      2014-04-23 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Intratumoral heterogeneity of gene amplification of HER2 in gastric cancers examined by MLPA and FISH.2013

    • Author(s)
      Akishi Ooi, Ryousuke Tajiri, Ritsuko Nakamura, Takeru Oyama ,Takashi Fujimura, Yoh Dobashi
    • Organizer
      72th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Related Report
      2013 Research-status Report
  • [Presentation] Global transcript analysos for the gastric carcinomas with intratumoral heterogeneity.2013

    • Author(s)
      Takeru Oyama, Ritsuko Nakamura, Ryosuke Tajiri, Akishi Ooi
    • Organizer
      72th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Related Report
      2013 Research-status Report
  • [Presentation] Nell 1/2 expression and function in kidney cancer.2013

    • Author(s)
      Ritsuko Nakamura, Takeru Oyama, Ryosuke Tajiri, Akishi Ooi
    • Organizer
      72th Annual Meeting of the Japanese Cancer Association.
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Related Report
      2013 Research-status Report
  • [Presentation] Utility of fluorescence in situ hybridization (FISH) to detect MDM2 amplification2013

    • Author(s)
      大井章史、土橋洋、野島孝之、池田博子、北村星子、尾山武
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      さっぽろ芸文館、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨軟部腫瘍におけるAKT遺伝子の異常と蛋白活性化2013

    • Author(s)
      土橋洋、蛭田昌宏、椙村春彦、山田茂樹、大井章史
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      さっぽろ芸文館、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] ERBB2増幅陽性胃癌における癌遺伝子増幅に関する腫瘍内多様性の検討2013

    • Author(s)
      田尻亮輔、尾山武、中村律子、大井章史
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      さっぽろ芸文館、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト胃癌でのHER2遺伝子発現の腫瘍内不均一性を示す部分における遺伝子発現2013

    • Author(s)
      尾山武、中村律子、田尻亮輔、大井章史
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      ロイトン札幌、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 胎児腎、腎癌などにおける分泌型タンパクNellの発現機能解析2013

    • Author(s)
      中村律子、尾山武、田尻亮輔、中本賢、大井章史
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      ロイトン札幌、札幌
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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