Semi-comorehensive analysis of gene amplification in solid carcinomas using MLPA and FISH
| Project/Area Number |
25460452
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kanazawa University |
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Principal Investigator |
Ooi Akishi 金沢大学, 医学系, 教授 (50160411)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 遺伝子増幅 / MLPA / FISH / 分子標的療法 / 胃癌 / 乳癌 / 大腸癌 / 脂肪肉腫 / 受容体チロシンキナーゼ / gene amplification / gastric cancer / ERBB2 / EGFR / FGFR2 / MYC |
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| Outline of Final Research Achievements |
Gene amplification is a major type of genetic aberration found in human cancers of solid types. The amplified genes so far have been useful targets or will be potential targets for molecular therapies. In this study, cancers of the stomach, breast and colon, which are frequent among Japanese, were examined for amplification of 46 genes by multiplex ligatioin-dependent probe amplification followed by fluoresence in situ hybridization. The results showed that the genes coding receptor tyrosine kinases (ERBB2, FGFR1, FGFR2, EGFR, MET), regulatory genes of cell cycle progression (CCNE1, CCND1, CDK6, MDM2), and gene coding a transcription factor (MYC) were found frequently amplified in these tumors.
In conclusion, combination of MLPA and FISH analysis is a feasible approach for obtaining the semi-comprehensive genetic information that is necessary for personalized molecular targeted therapy.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Intratumoral heterogeneous amplification of ERBB2 and subclonal genetic diversity in gastric cancers revealed by multiple ligation-dependent probe amplification and fluorescence in situ hybridization.2014
Author(s)
Tajiri. R., Ooi. A., Fujimura, T., Dobashi, Y., Oyama, T., Nakamura, R. and Ikeda, H.
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Journal Title
Hum Pathol.
Volume: 45(4)
Issue: 4
Pages: 725-34
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] ＭLPAとFISHを用いた胃癌における増幅遺伝子の準網羅的検索2014
Author(s)
大井 章史、田尻 亮輔、尾山 武、中村 律子、伏田 幸夫、土橋 洋
Organizer
73th Annual Meeting of the Japanese Cancer Association
Place of Presentation
PACIFICO YOKOHAMA, Yokohama
Year and Date
2014-09-25 – 2014-09-27
Related Report
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