Virulence mechanism of atopic dermatitis-related S. aureus
Project/Area Number |
25460533
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bacteriology (including mycology)
|
Research Institution | Hiroshima University |
Principal Investigator |
Sugai Motoyuki 広島大学, 医歯薬保健学研究院(歯), 教授 (10201568)
|
Co-Investigator(Kenkyū-buntansha) |
HISATSUNE JUNZO 広島大学, 大学院医歯薬保健学研究院, 助教 (40513180)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | S. aureus / アトピー性皮膚炎 / 黄色ブドウ球菌 / フィラグリン / 比較ゲノム / 菌固着 |
Outline of Final Research Achievements |
Comparison of adherent bacterial number of AD-derived S. aureus and non AD-derived S. aureus to the skin of FKO mouse indicated AD-derived S. aureus adhered more to the skin. We established an expression plasmid carrying sarA P1 promoter and RBS sequence of sod gene hooked with gfp and transformed AD-deribed S. aureus. We also finished complete DNA sequencing of TF3378 (AD-derived) genome. Comparison with other finished sequences of S. aureus revealed some potential virulence-related genes specific to AD-derived S. aureus.
|
Report
(4 results)
Research Products
(14 results)
-
-
[Journal Article] Inhibitory effects of antibiofilm compound 1 against Staphylococcus aureus biofilms.2016
Author(s)
Shrestha L, Kayama S, Sasaki M, Kato F, Hisatsune J, Tsuruda K, Koizumi K, Tatsukawa N, Yu L, Takeda K, Sugai M.
-
Journal Title
Microbiology and Immunology
Volume: 60
Issue: 3
Pages: 148-159
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Application of moisturizer to neonates prevents development of atopic dermatitis.2014
Author(s)
Horimukai K, Morita K, Narita M, Kondo M, Kitazawa H, Nozaki M, Shigematsu Y, Yoshida K, Niizeki H, Motomura K, Sago H, Takimoto T, Inoue E, Kamemura N, Kido H, Hisatsune J, Sugai M, Murota H, Katayama I, Sasaki T, Amagai M, Morita H, Matsuda A, Matsumoto K, Saito H, Ohya Y.
-
Journal Title
The Journal of allergy and clinical immunology.
Volume: 134
Issue: 4
Pages: 824-830
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] A novel metallo-β-lactamase, IMP-34, in Klebsiella isolates with decreased resistance to imipenem.2013
Author(s)
Shigemoto N, Kayama S, Kuwahara R, Hisatsune J, Kato F, Nishio H, Yamasaki K, Wada Y, Sueda T, Ohge H, Sugai M
-
Journal Title
Diagnostic Microbiology and Infectious Disease
Volume: 76
Issue: 1
Pages: 119-121
DOI
Related Report
Peer Reviewed
-
[Journal Article] Identification and characterization of a novel aac(6’)-lag associated with the blaIMP-1-integron in a multidrug-resistant Pseudomonas aeruginosa2013
Author(s)
Kobayashi K, Hayashi I, Kouda S, Kato F, Fujiwara T, Kayama S, Hirakawa H, Itaha H, Ohge H, Gotoh N, Usui T, Matsubara A, Sugai M
-
Journal Title
PLoS One
Volume: 8
Issue: 8
Pages: e70557-e70557
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-