| Project/Area Number |
25460538
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHOBUIKE TAKEO 佐賀大学, 医学部, 助教 (70336113)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | レジオネラ / 細胞内寄生 / インフラマソーム / naip / パイロトーシス / アポトーシス / 自然免疫 / Naip / pyroptosis |
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| Outline of Final Research Achievements |
Naip5 is a natural resistance gene against Legionella infection in mice. We examined the functional differences among three Naip5 alleles. C57BL/6 Naip5 restricts intracellular growth of Legionella, while A/J Naip5 do not, in RAW264.7 macrophages. Effect of Balb/c Naip5 was intermediate. After L. pneumophila infection, C57BL/6 and A/J Naip5 significantly promoted cell death, which was not observed in cells expressing Balb/c Naip5. C57BL/6 Naip5 induced caspase-1 activation in infected cells, whereas no substantial activation was observed in Balb/c and A/J Naip5-expressing cells. Apoptosis upon DNA damage was inhibited by C57BL/6 and Balb/c Naip5, while A/J Naip5 did not affect. Interestingly, C57BL/6 Naip5 significantly promoted, whereas Balb/c Naip5 inhibited, cell death induced by reactive oxygen species. These results indicate that Naip5 regulate cell survival by inhibiting apoptosis or by promoting pyroptosis in response to specific cellular signals.
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