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Studies on the mechanisms involved in the host range restriction of influenza A viruses

Research Project

Project/Area Number 25460581
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionThe University of Tokyo (2015)
Iwate University (2013-2014)

Principal Investigator

Imai Masaki  東京大学, 医科学研究所, 准教授 (30333363)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords鳥インフルエンザウイルス / ヘマグルチニン(HA)蛋白質 / 安定性 / レセプター特異性 / 膜融合活性 / 感染性 / 熱安定性 / 酸性pH / 季節性インフルエンザウイルス / 構造安定性
Outline of Final Research Achievements

A change in the receptor binding properties of the hemagglutinin (HA) of avian influenza viruses is a critical step in their adaptation to human hosts. Indeed, the 1918 H1N1, 1957 H2N2, and 1968 H3N2 pandemic strains all exhibited human-type receptor-binding preference despite the fact that the HAs of these viruses were derived from avian species. To understand the mechanisms by which influenza viruses adapt to the human host, we analyzed the stability of HA proteins from H1, H2, and H3 avian influenza viruses, which possess mutations that confer human-type receptor recognition. We observed that mutations conferring human-type receptor recognition decreased slightly the HA protein stability of H1 avian virus, but not affect the HA stability of H2 and H3 avian viruses. These results suggest that H2 and H3 avian influenza viruses may be relatively easily able to acquire the mutation(s) necessary to confer human-type receptor recognition

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Invited: 2 results)

  • [Journal Article] Identification of Stabilizing Mutations in an H5 HA Influenza Virus Protein.2015

    • Author(s)
      Hanson A, Imai M, Hatta M, McBride R, Imai H, Taft A, Zhong G, Watanabe T, Suzuki Y, Neumann G, Paulson JC, Kawaoka Y.
    • Journal Title

      Journal of Virology

      Volume: 90 Issue: 6 Pages: 2981-2990

    • DOI

      10.1128/jvi.02790-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of high-yield influenza A virus vaccine viruses2015

    • Author(s)
      Ping J, Lopes TJ, Nidom C, Ghedin E, Macken C, Fitch A, Imai M, Maher E, Neumann G & Kawaoka Y
    • Journal Title

      Nat Commun

      Volume: 6 Issue: 1 Pages: 8148-8148

    • DOI

      10.1038/ncomms9148

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel residues in avian influenza virus PB2 protein affect virulence in mammalian hosts.2014

    • Author(s)
      Fan S, Hatta M, Kim JH, Halfmann P, Imai M, Macken CA, Le MQ, Nguyen T, Neumann G, Kawaoka Y
    • Journal Title

      Nature communications

      Volume: 5 Issue: 1 Pages: 5021-5021

    • DOI

      10.1038/ncomms6021

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Circulating avian influenza viruses closely related to the 1918 virus have pandemic potential.2014

    • Author(s)
      Watanabe T, Zhong G, Russell CA, Nakajima N, Hatta M, Hanson A, McBride R, Burke DF, Takahashi K, Fukuyama S, Tomita Y, Maher EA, Watanabe S, Imai M, Neumann G, Hasegawa H, Paulson JC, Smith DJ, Kawaoka Y
    • Journal Title

      Cell Host and Microbe

      Volume: 15 Issue: 6 Pages: 692-705

    • DOI

      10.1016/j.chom.2014.05.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Avian influenza virus transmission to mammals.2014

    • Author(s)
      Herfst S, Imai M, Kawaoka Y , Fouchier RA
    • Journal Title

      Current Topics in Microbiology and Immunology

      Volume: 385 Pages: 137-155

    • DOI

      10.1007/82_2014_387

    • ISBN
      9783319111544, 9783319111551
    • Related Report
      2014 Research-status Report
  • [Journal Article] Transmission of influenza A/H5N1 viruses in mammals.2013

    • Author(s)
      Imai M, Herfst S, Sorrell EM, Schrauwen EJ, Linster M, De Graaf M, Fouchier RA, Kawaoka Y
    • Journal Title

      Virus Research

      Volume: 178 Issue: 1 Pages: 15-20

    • DOI

      10.1016/j.virusres.2013.07.017

    • Related Report
      2013 Research-status Report
  • [Journal Article] Selection on haemagglutinin imposes a bottleneck during mammalian transmission of reassortant H5N1 influenza viruses.2013

    • Author(s)
      Wilker PR, Dinis JM, Starrett G, Imai M, Hatta M, Nelson CW, O'Connor DH, Hughes AL, Neumann G, Kawaoka Y, Friedrich TC
    • Journal Title

      Nature communications

      Volume: 4 Issue: 1 Pages: 2636-2636

    • DOI

      10.1038/ncomms3636

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of H7N9 influenza A viruses isolated from humans.2013

    • Author(s)
      Watanabe T, Kiso M, Fukuyama S, Nakajima N, Imai M, Yamada S, Murakami S, Yamayoshi S, Iwatsuki-Horimoto K, Sakoda Y, Takashita E, McBride R, Noda T, Hatta M, Imai H, Zhao D, Kishida N, Shirakura M, de Vries RP, Shichinohe S, Okamatsu M, Tamura T, Tomita Y , Fujimoto N, Goto K, Katsura H, Kawakami E, Ishikawa I, Watanabe S, Ito M, Sakai-Tagawa Y, Sugita Y, Uraki R, Yamaji R, Eisfeld AJ, Zhong G, Fan S, Ping J, Maher EA, Hanson A, Uchida Y, Saito T, Ozawa M, Neumann G, Kida H, Odagiri T, Paulson JC, Hasegawa H, Tashiro M, Kawaoka Y
    • Journal Title

      Nature

      Volume: 501(7468) Issue: 7468 Pages: 551-5

    • DOI

      10.1038/nature12392

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 中国で発生した鳥インフルエンザA(H7N9)について2013

    • Author(s)
      今井正樹
    • Organizer
      第47回日本ウイルス学会北海道支部会夏季シンポジウム
    • Place of Presentation
      ホテル北乃湯(北海道奈井江町)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 空気伝播するH5インフルエンザウイルス2013

    • Author(s)
      今井正樹
    • Organizer
      第127回 宮崎大学農学部獣医学科集談会
    • Place of Presentation
      宮崎大学農学部(宮崎県宮崎市)
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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