| Project/Area Number |
25460695
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | National Institute of Health Sciences (2016)
Yokohama City University (2013-2015) |
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Principal Investigator |
ARAKAWA Noriaki 国立医薬品食品衛生研究所, 医薬安全科学部, 主任研究官 (60398394)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAGI Etsuko 横浜市立大学, 医学部産婦人科, 教授 (40275053)
MIYAGI Etsuko 福島県立医科大学, 医学部衛生学・予防医学講座, 助教 (20626767)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 卵巣がん / 明細胞がん / TFPI2 / バイオマーカー / 診断 / 卵巣癌 / 明細胞癌 / 診断マーカー / 明細胞腺癌 / 遺伝子発現 / プロモーターメチル化 / 組織因子経路インヒビター２ |
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| Outline of Final Research Achievements |
In this study, we examined relationship between TFPI2 and OCCC by investigating mechanisms underlying blood expression of TFPI2 in OCCC patients, and sought to evaluate the serum biomarker performance of TFPI2 in preoperative prediction of OCCC. TFPI2 gene was highly expressed in tumor tissues from OCCC patients. Unlike other cancer cell lines, and hypermethylation of TFPI2 promoter was undetected in OCCC-derived cell lines. Gene knockdown experiments showed that p53 is a more likely candidate for transcription factor involved in the characteristic expression of TFPI2 in OCCC. Serum TFPI2 was specifically elevated in OCCC patients, consistent with the gene expression level in the tumor tissues. These results suggest that TFPI2 is a useful serum biomarker for clinical diagnosis of OCCC.
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