The development of novel, quick, and simple FRET-based telomerase assay method
| Project/Area Number |
25460708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Konan University |
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Principal Investigator |
MURASHIMA TAKASHI 甲南大学, フロンティアサイエンス学部, 教授 (20263923)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 凝集誘起発光 / テロメア / アミロイドβ / ガン / アルツハイマー病 / 早期診断 / アミロイド / テロメラーゼ / 蛍光共鳴エネルギー移動 / ガン診断 / TRAP assay / G-quadruplex |
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| Outline of Final Research Achievements |
A simple and sensitive methods for measuring telomeric tandem repeat DNA and amyloid-β based on AIE phenomena were developed. According to these methods, telomere concentration can be estimated with high sensitivity (5 pM for 12-mer telomeric sequences) using AIE-CCCTAACCCTAA probe. On the other hand, the detection of Aβ was performed with AIE dye-amyloid fibrillization-promoting peptide (AFPP) probe. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM.
This method provides a simple, rapid, sensitive and cost-effective assay for telomere sequence and Aβ.
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Report
(4 results)
Research Products
(30 results)
