| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
A simple and sensitive methods for measuring telomeric tandem repeat DNA and amyloid-β based on AIE phenomena were developed. According to these methods, telomere concentration can be estimated with high sensitivity (5 pM for 12-mer telomeric sequences) using AIE-CCCTAACCCTAA probe. On the other hand, the detection of Aβ was performed with AIE dye-amyloid fibrillization-promoting peptide (AFPP) probe. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM.
This method provides a simple, rapid, sensitive and cost-effective assay for telomere sequence and Aβ.
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