Epidemiological study on the genetic polymorphisms and methylation effects of physical activity for lifestyle disease prevention

• Project/Area Number: 25460759
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Saga University
• Principal Investigator: Hara Megumi 佐賀大学, 医学部, 准教授 (90336115)
• Co-Investigator(Kenkyū-buntansha): 田中 恵太郎 佐賀大学, 医学部, 教授 (50217022) ; 西田 裕一郎 佐賀大学, 医学部, 講師 (50530185)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 遺伝子損傷 / 身体活動 / メチル化 / 遺伝子修復酵素 / がん / 身体活動量 / 遺伝子多型

Outline of Final Research Achievements

Genetic damage due to reactive oxygen species increases with age and contributes to the onset of cancer. In this study, we examined the relationship between physical activity and DNA damage, and DNA damage repair gene methylation. The concentration of urinary 8-hydroxdeoxyguanosine (8-OHdG), a DNA damage marker, was negatively correlated with the total body activity level in females and negatively correlated with mid- to high-intensity physical activity levels in males. On the other hand, DNA methylation in the promoter region of 8-oxoguanine DNA glycosylase 1 (hOGG1), a gene damage repair enzyme, was positively correlated with the amount of physical activity. Higher amount of physical activity increases endogenous antioxidant capacity and DNA damage decreases. Conversely, DNA damage increases when the amount of physical activity is low, so the promoter region of DNA is low in order to express hOGG1 Suggesting the possibility of methylation.

Research Products

• [Journal Article] Intensity-specific effect of physical activity on urinary levels of 8-hydroxydeoxyguanosine in middle-aged Japanese (2016)
  • Author(s): Hara M, Nishida Y, Shimanoe C, et. al.
  • Journal Title: Cancer Science Volume: 107 Issue: 11 Pages: 1653-1659
  • DOI: 10.1111/cas.13070
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Factors Associated With Non-participation in a Face-to-Face Second Survey Conducted 5 Years After the Baseline Survey (2015)
  • Author(s): Hara M, Shimanoe C, Otsuka Y, Nishida Y, Nanri H, Horita M, Yasukata J, Miyoshi N, Yamada Y, Higaki Y, Tanaka K.
  • Journal Title: Journal of Epidemiology Volume: 25 Issue: 2 Pages: 117-125
  • DOI: 10.2188/jea.JE20140116
  • NAID: 130004704585
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Associations Between <i>hOGG1</i> Ser326Cys Polymorphism and Increased Body Mass Index and Fasting Glucose Level in the Japanese General Population (2014)
  • Author(s): Hara M, Nakamura K, Nanri H, Nishida Y, Hishida A, Kawai S, Hamajima N, Kita K, Suzuki S, Mantjoro E M, Ohnaka K, Uemura H, Matsui D, Oze I, Mikami H, Kubo M and Tanaka H on behalf of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group.
  • Journal Title: Journal of Epidemiology Volume: 24 Issue: 5 Pages: 379-384
  • DOI: 10.2188/jea.JE20140002
  • NAID: 130004449419
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Associations between hOGG1 Ser326Cys polymorphism and increased body mass index and fasting glucose in the Japanese general population. (2014)
  • Author(s): Hara M, Nakamura K, Nanri H, Nishida Y, Hishida A, Kawai S, Hamajima N, Kita K, Suzuki S, Mantjoro E M, Ohnaka K, Uemura H, Matsui D, Oze I, Mikami H, Kubo M and Tanaka H
  • Journal Title: J Epidemiol Volume: 未定
  • Peer Reviewed
• [Journal Article] Objectively measured physical activity and inflammatory cytokine levels in middle-aged Japanese people (2014)
  • Author(s): Nishida Y, Higaki Y, Taguchi N, Hara M, Nakamura K, Nanri H, Imizumi T, Sakamoto T, Horita M, Shinchi K, Tanaka K
  • Journal Title: Preventive Medicine Volume: 未定
  • Peer Reviewed
• [Journal Article] Gender-specific associations of perceived stress and coping strategies with C-reactive protein in middle-aged and older men and women (2014)
  • Author(s): Shimanoe C, Otsuka Y, Hara M, Nanri H, Nishida Y, Nakamura K, Higaki Y, Imaizumi T, Taguchi N, Sakamoto T, Horita M, Shinchi K, Tanaka K
  • Journal Title: International Journal of Behavioral Medicine Volume: 未定 Issue: 5 Pages: 821-832
  • DOI: 10.1007/s12529-013-9341-y
  • Peer Reviewed
• [Presentation] ミトコンドリアハプログループDが身体活動量と尿中8OHdG濃度の関連に及ぼす影響 (2017)
  • Author(s): 原めぐみ、西田裕一郎、福典之、田口尚人、島ノ江千里、堀田美加子、河井一明、李 云善、葛西順、葛西宏、檜垣靖樹、田中恵太郎
  • Organizer: 第27回日本疫学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2017-01-25
• [Presentation] 加齢とhOGG1遺伝子プロモーター領域のメチル化の関連 (2016)
  • Author(s): 原 めぐみ、西田 裕一郎、島ノ江 千里、堀田 美加子、田中 恵太郎
  • Organizer: 第26回日本疫学会学術総会
  • Place of Presentation: 米子
  • Year and Date: 2016-01-21
• [Presentation] Association between moderate/vigorous intensity of physical activity and urinary levels of 8-hydroxydeoxyguanosine (2015)
  • Author(s): Megumi Hara, Yuichiro Nishida, Chisato Shimanoe, Yasuko Otsuka, Hinako Nanri, Yasuki Higaki, Mikako Horita, Kazuaki Kawai, Yun-Shan Li, Jun Kasai, Hiroshi Kasai, Keitaro Tanaka
  • Organizer: The 25th Annual Scientific Meeting of the Japan Epidemiological Association
  • Place of Presentation: Nagoya
  • Year and Date: 2015-01-22 – 2015-01-23
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野
  • URL: http://www.prevent.med.saga-u.ac.jp/index.html
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野 研究業績
  • URL: http://www.prevent.med.saga-u.ac.jp/gyoseki.html
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野 業績
  • URL: http://www.prevent.med.saga-u.ac.jp/lecture.html
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野 原めぐみ
  • URL: http://www.prevent.med.saga-u.ac.jp/hara.html
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野
  • URL: http://www.prevent.med.saga-u.ac.jp/index.html