| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we investigated the mechanisms of Fosb gene expression focused on epigenetic regulation by arsenic in mouse hepatoma Hepa1c1c7 cells. Upon exposure of Hepa1c1c7 cells to 0.5 μM As2O3, the expression of Fosb was greatly increased after 2 days. The bisulfite sequencing revealed that DNA methylation level decreased in the DNA regions around transcription start sites (TSS) of Fosb gene by arsenic. We also found a decrease of DNA methylation level in the same DNA region and an increase of Fosb gene expression by DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine and siDnmt3b in Hepa1c1c7 cells. These results suggested that the DNA methylation change in the DNA regions around TSS of Fosb gene by Dnmt3b is involved in its expression regulation by arsenic in Hepa1c1c7 cells.
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