Studies on the regulation of Fos family gene expression involved in late-onset increases of tumor induced by arsenic
| Project/Area Number |
25460831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
Suzuki Takehiro 国立研究開発法人国立環境研究所, 環境健康研究センター, 主任研究員 (60425494)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヒ素 / エピジェネティクス / Fosb |
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| Outline of Final Research Achievements |
In this study, we investigated the mechanisms of Fosb gene expression focused on epigenetic regulation by arsenic in mouse hepatoma Hepa1c1c7 cells. Upon exposure of Hepa1c1c7 cells to 0.5 μM As2O3, the expression of Fosb was greatly increased after 2 days. The bisulfite sequencing revealed that DNA methylation level decreased in the DNA regions around transcription start sites (TSS) of Fosb gene by arsenic. We also found a decrease of DNA methylation level in the same DNA region and an increase of Fosb gene expression by DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine and siDnmt3b in Hepa1c1c7 cells. These results suggested that the DNA methylation change in the DNA regions around TSS of Fosb gene by Dnmt3b is involved in its expression regulation by arsenic in Hepa1c1c7 cells.
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Report
(4 results)
Research Products
(26 results)
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[Presentation] 胎児期ヒ素曝露が標的臓器に及ぼす影響2014
Author(s)
内匠正太, 岡村和幸, 鈴木武博, 羽野寛, 野原恵子, 柳澤裕之
Organizer
第84回日本衛生学会学術総会
Place of Presentation
岡山コンベンションセンター（岡山県岡山市）
Year and Date
2014-05-25 – 2014-05-27
Related Report
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[Book] 毒性の科学2014
Author(s)
鈴木武博, 野原恵子
Total Pages
200
Publisher
東大出版会
Related Report
